A5059840599- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Herpesvirus Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Insect and Pesticide Research
- Food Allergy and Anaphylaxis Research
- Coccidia and coccidiosis research
- Microbial infections and disease research
- SARS-CoV-2 and COVID-19 Research
- Contact Dermatitis and Allergies
- Plant Virus Research Studies
- Italian Fascism and Post-war Society
- Virology and Viral Diseases
- Microscopic Colitis
- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Toxoplasma gondii Research Studies
- Viral Infections and Outbreaks Research
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2011-2024
University of Padua
2024
Warsaw University of Life Sciences
2024
National Influenza Center
2024
University of Minnesota
2015
World Health Organization - Italy
2015
San Raffaele University of Rome
1997
Abstract Porcine epidemic diarrhea virus (PEDV) has been detected sporadically in Italy since the 1990s. We report phylogenetic relationship of swine enteric coronaviruses collected during 2007–2014 and identify a drastic shift PEDV strain variability new coronavirus generated by recombination transmissible gastroenteritis PEDV.
Porcine circovirus type 3 (PCV3) was recently proposed as a new porcine circovirus. It has been described by researchers in the USA and China associated with dermatitis nephropathy syndrome, reproductive failure systemic inflammation disease. The study reports occurrence of virus Italy. PCV3 detected tissues foetuses stillborn piglets coming from two farms located Po Valley. genome sequences Italian strains share 99.7% to 97.8% nucleotide identity those available GenBank. Results strengthen...
Influenza D virus (IDV), a new member of the Orthomyxoviridae family, was first reported in 2011 swine Oklahoma, and consequently found cattle across North America Eurasia. To investigate circulation IDV among pigs Italy, period between June 2015 May 2016, biomolecular virological tests were performed on 845 clinical samples collected from 448 pig farms affected by respiratory distress located Po Valley. Serological conducted 3698 sera, including archive sera 2009, as well same region. Viral...
Porcine Epidemic Diarrhoea Virus (PEDV) causes watery diarrhoea, dehydration, and a high mortality rate among suckling pigs. Recently, PEDV had large negative economic impact on the swine industries in Asia North America. In 2014, re-emerged many European countries, but most countries only reported few sporadic cases. Here, we report epidemic wave that occurred Italy from 2015 to 2017. During this time, was detected by real-time PCR 438 farms located mainly high-density pig production area...
Influenza D virus (IDV) has been increasingly reported all over the world. Cattle are considered major viral reservoir. Based on hemagglutinin-esterase (HEF) gene, three main genetic and antigenic clusters have identified: D/OK distributed worldwide, D/660 detected only in USA D/Japan Japan. Up to 2017, Italian IDV isolates belonged cluster. From January 2018 May 2019, we performed virological surveillance for from respiratory outbreaks 725 bovine farms Northern Italy by RT-PCR. Seventy-four...
Tissue and blood samples were collected from 152 wild boars (Sus scrofa) the Maremma area (Grosseto district, Central Italy) between November 2002 January 2003. The presence of pseudorabies virus (PRV) antibodies, antigen, DNA confirmed by an enzyme-linked immunosorbent assay (ELISA), immunohistochemistry, polymerase chain reaction (PCR), respectively. Of animals, 62 (41%) positive for viral antigen in tonsillar tissue. 80 serum that suitable testing, 41 (51%) PRV antibodies. Positive...
Swine play an important role in the ecology of influenza A viruses (IAVs), acting as mixing vessels. (sw) IAVs H1N1 (including H1N1pdm09), H3N2, and H1N2 subtypes are enzootic pigs globally, with different geographic distributions. This study investigated genetic diversity swIAVs detected during passive surveillance pig farms Northern Italy between 2017 2020. total 672 samples, IAV-positive according to RT-PCR, were subtyped by multiplex RT-PCR. selection strains was fully sequenced. High...
It has been observed that novel strains of Clostridioides difficile can rapidly emerge and move between animal human hosts. The aim this study was to investigate the prevalence C. in pigs dairy cattle northern Italy characterize compare with those from patients same geographical area. were isolated animals farms slaughterhouses (cross-sectional studies) neonatal enteric disorders routine diagnostic investigations (passive surveillance). Samples positive for found 87% pig 40% involved...
Summary Influenza A virus ( IAV ) infection in swine plays an important role the ecology of influenza viruses. The emergence new s comes through different mechanisms, with genetic reassortment genes between viruses, also originating from species, being common. We performed a analysis on 179 isolates humans (n. 75) and pigs 104) collected Northern Italy 2010 2015, to monitor exchange human s. No cases strains were noticed, but direct infections H1N1pdm09 detected. Moreover, we pointed out...
Pollen from perennial rye grass (Lolium perenne), a major cause of type-I allergy worldwide, contains complex mixture allergenic proteins among which Lol p 1 is one the most important. We describe expression, purification and characterization recombinant overproduced in Escherichia coli. The allergen, expressed high yields purified milligram amounts, bound to specific IgE antibodies human sera, induced histamine release sensitized basophils, elicited rabbit antisera that recognize...
The present study retrospectively analyzed the infectious agents associated with 829 abortion outbreaks occurring from 2011 to 2021 in northern Italy. Foetuses were subjected necropsies, and organ samples by direct PCR screen for six swine pathogens. In 42.0% of examined outbreaks, at least one agent was found. Porcine reproductive respiratory syndrome virus (PRRSV) (24.9%) porcine circovirus-2 (PCV2) (11.5%) most frequently detected among known abortion-inducing Chlamydia spp. (5.6%),...
Recent studies have identified a new genus of the Orthomyxoviridae family, Influenza D virus (IDV). This was shown to infect farm animals including swine and cattle, efficiently replicate transmit in ferrets, animal model choice for transmission influenza A humans. partnering grant on IDV addressed need capacity building at EU level improve EU's scientific assessment international competitiveness. We promoted cross-disciplinary cooperation between partner institutes representing six Member...
Recent studies have identified a new genus of the Orthomyxoviridae family, Influenza D virus (IDV). This was shown to infect farm animals including swine and cattle, efficiently replicate transmit in ferrets (Hause et al., 2013; Liu 2020), animal model choice for transmission influenza A humans. partnering grant (Flu-D project) on IDV addressed need capacity building at EU level improve EU's scientific assessment international competitiveness. We promoted cross-disciplinary cooperation...
<title>Abstract</title> Porcine Reproductive and Respiratory Syndrome (PRRS) causes huge economic losses to pig farms worldwide. Currently available vaccines do not always offer complete protection, due the extreme variability of virus. Therefore, good farming practices must be improved prevent disease from spreading across production system. In this study, we inferred dynamics PRRSV population in Italy by applying bayesian methods on our ORF7 sequence dataset collected during a 15-year...
Abstract The influenza A H1N1pdm09 virus continues to be a significant pathogen, posing potential risks both animal and human health due its zoonotic potential. Italy, which has one of the largest swine populations in Europe, plays crucial role monitoring evolution viruses livestock. This study aims address existing knowledge gaps regarding genetic diversity transmission dynamics circulating Italian populations. Utilizing whole genome sequencing dynamic modeling, we conducted comprehensive...
Quantitative real-time polymerase chain reaction (PCR) has become an important tool for Porcine circovirus-2 (PCV-2) research and diagnosis. However, significant differences in detection limit quantification data, among laboratories quantitative PCR methods, have been demonstrated. New efforts are required providing more accurate comparable results. The current study is evaluation of the effects DNA extraction procedures on PCV-2 lymph node samples. Differences, greater than 1 log 10...