A5001535548- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Viral Infectious Diseases and Gene Expression in Insects
- Cytomegalovirus and herpesvirus research
- Vector-borne infectious diseases
- Insect and Pesticide Research
- Microbial infections and disease research
- Plant Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Respiratory viral infections research
- T-cell and Retrovirus Studies
- Mosquito-borne diseases and control
- Poxvirus research and outbreaks
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis Viruses Studies and Epidemiology
- Veterinary medicine and infectious diseases
- CRISPR and Genetic Engineering
- RNA regulation and disease
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2016-2025
University of Turin
2021
The Pirbright Institute
2007
University of Milan
1994
Hungarian Academy of Sciences
1992
Istituto Zooprofilattico Sperimentale delle Venezie
1991-1992
Neutralizing monoclonal antibodies (nMAbs) elicited against foot-and-mouth disease virus (FMDV) of serotype C were assayed with field isolates and variant FMDVs using several immunoassays. Of a total 36 nMAbs tested, 23 recognized capsid protein VP1 distinguished at least 13 virion conformation- independent epitopes involved in neutralization FMDV C. Eleven C-S8cl have been located segments 138–156 or 192–209 by quantifying the reactivity synthetic peptides nMAb-resistant mutants carrying...
The interferon (IFN) system is a major effector of the innate immunity that allows time for subsequent establishment an adaptive immune response against wide-range pathogens. Their diverse biological actions are thought to be mediated by products specific but usually overlapping sets cellular genes induced in target cells. Ubiquitin ligase members tripartite motif (TRIM) protein family have emerged as IFN-induced proteins involved both and immunity. In this report, we provide evidence TRIM22...
ABSTRACT Foot-and-mouth disease virus (FMDV), the causative agent of foot-and-mouth disease, is an Apthovirus within Picornaviridae family. Replication occurs in association with replication complexes that are formed by host cell membrane rearrangements. The largest viral protein complex, 2C, thought to have multiple roles during replication. However, studies examining function FMDV 2C been rather limited. To better understand role process replication, we used a yeast two-hybrid approach...
Foot-and-mouth disease (FMD) is a highly contagious of livestock affecting animal production and trade throughout Asia Africa. Understanding FMD virus (FMDV) global movements evolution can help to reconstruct the spread between endemic regions predict risks incursion into FMD-free countries. Global expansion single FMDV lineage rare but result in severe economic consequences. Using extensive sequence data we have reconstructed space-time transmission history O/ME-SA/Ind-2001 (which normally...
Abstract Orthobunyaviruses (OBVs) form a distinct genus of arthropod-borne bunyaviruses that can cause severe disease upon zoonotic transmission to humans. Antigenic drift or genome segment re-assortment have in the past resulted new pathogenic OBVs, making them potential candidates for causing emerging zoonoses future. Low-resolution electron cryo-tomography studies shown OBV particles feature prominent trimeric spikes, but their molecular organization remained unknown. Here we report X-ray...
Lumpy skin disease is a high-consequence in cattle caused by infection with the poxvirus lumpy virus (LSDV). The endemic most countries Africa and an emerging threat to populations Europe Asia. As LSDV spreads into new regions, it important that signs of are recognized promptly animal caregivers. This study describes gross, microscopic, ultrastructural changes occur over time experimentally challenged LSDV. Four calves were inoculated wildtype monitored for 19 21 days. At 7 days after...
We conducted a serologic survey among dogs and cats in Italy to detect antibodies against severe acute respiratory syndrome virus 2 (SARS-CoV-2). found that SARS-CoV-2 seroprevalence was higher (16.2%) than (2.3%). In addition, animals living close contact with SARS-CoV-2-positive owners.
Foot-and-mouth disease viruses are often restricted to specific geographical regions and spread new areas may lead significant epidemics. Phylogenetic analysis of sequences the VP1 genome region recent outbreak from Libya Saudi Arabia has revealed a lineage, O-Ind-2001, normally found in Indian subcontinent. This paper describes characterization field collected these cases provides information about real-time RT-PCR assay that can be used detect this lineage discriminate them other endemic...
An increase in autochthonous hepatitis E virus (HEV) infections has been recorded Italy suspected to be zoonotically transmitted from pigs; this study was carried out determinate the seroprevalence and risk factors associated with HEV exposition, both swine humans working pig farms, located within a high-density farming area Piedmont region, north-western Italy. The presence of viral RNA human samples also evaluated, phylogenetic analysis performed on HEV-positive samples. Forty-two farms...
Nonstructural protein 3A of foot-and-mouth disease virus (FMDV) is a partially conserved 153 amino acids in most FMDVs examined to date. The role growth and virulence within the natural host not well understood. Using yeast two-hybrid approach, we identified cellular DCTN3 as specific binding partner for 3A. subunit dynactin complex, cofactor dynein, motor protein. dynactin-dynein duplex has been implicated several subcellular functions involving intracellular organelle transport. 3A-DCTN3...
Knowledge of the antigenic structure foot-and-mouth disease virus (FMDV) has relevance in development diagnostic assays, evaluation variability and selection appropriate vaccine strains. Antigenic sites have been investigated only FMDVs serotypes O, A C, while it would be valuable to extend studies also other serotypes. This paper reports identification involved neutralization FMDV serotype Asia 1 by using a new panel mAbs their relation with described is discussed. Out 24 raised against 1,...
Lumpy skin disease (LSD) is an infectious affecting bovine with severe symptomatology. The implementation of effective control strategies to prevent infection outbreak requires rapid diagnostic tools. Two monoclonal antibodies (mAbs), targeting different epitopes the LSDV structural protein p32, and gold nanoparticles (AuNPs) were used set up a colorimetric sandwich-type lateral flow immunoassay (LFIA). Combinations including one or two mAbs, either as capture detection reagent, explored...