A5085474747- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Analytical chemistry methods development
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Mycotoxins in Agriculture and Food
- Viral Infections and Immunology Research
- Pesticide Residue Analysis and Safety
- Vector-Borne Animal Diseases
- Microfluidic and Capillary Electrophoresis Applications
- Antibiotics Pharmacokinetics and Efficacy
- Mass Spectrometry Techniques and Applications
- Heavy metals in environment
- Forensic Toxicology and Drug Analysis
- Polymer Surface Interaction Studies
- Microbial infections and disease research
- Analytical Chemistry and Chromatography
- Salivary Gland Disorders and Functions
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Adsorption and biosorption for pollutant removal
- Nanocluster Synthesis and Applications
- Listeria monocytogenes in Food Safety
University of Turin
2018-2025
Institut Agro Montpellier
2023
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2023
UMR QualiSud
2023
Université de Montpellier
2023
Institut de Recherche pour le Développement
2023
Torino e-district
2020
Stable and efficient conjugates between antibodies gold nanoparticles (GNP-Ab) are sought to develop highly sensitive robust biosensors with applications in medicine, toxicology, food safety controls, targeted drug delivery. Several strategies have been proposed for directing the antibody attachment GNPs thus preserving activity, including covalently coupling a polymer grafted on GNP surface exploiting high affinity of bioreceptors as mediators binding. Both approaches also allow shielding...
The COVID-19 pandemic highlighted lateral flow immunoassay (LFIA) strips as the most known point-of-care (POC) devices enabling rapid and easy detection of relevant biomarkers by nonspecialists. However, these diagnostic tests are usually associated with qualitative biomarker interest. Alternatively, electrochemical-based diagnostics, especially for diabetes care, enable quantitative determination biomarkers. From an analytical point perspective, combination two approaches might represent a...
The cross-linker methylene-bis-acrylamide is usually present in nanoMIPs obtained by solid-phase polymerization synthesis at 2 mol% concentration, with very few exceptions. Here, we studied the influence of variable amounts range between 0 (no cross-linker) and 50 concentration on binding properties rabbit IgG nanoMIPs. parameters were determined equilibrium experiments results show that degree cross-linking defines three distinct types nanoMIPs: (i) those a low cross-linking, including...
Point-of-care diagnostic tests, such as lateral-flow immunoassay (LFIA), have emerged a fast tool in both human and veterinary medicine. In this paper, gold nanoparticle-based LFIA device was developed for the measurement of C-reactive protein (CRP) porcine saliva, using monoclonal anti-porcine CRP antibody. The dilution ratio saliva samples optimized at 1:5 with an assay buffer. reaction time to 20 min, since provided positive signal high concentration samples, but negative result buffer or...
Lumpy skin disease (LSD) is an infectious affecting bovine with severe symptomatology. The implementation of effective control strategies to prevent infection outbreak requires rapid diagnostic tools. Two monoclonal antibodies (mAbs), targeting different epitopes the LSDV structural protein p32, and gold nanoparticles (AuNPs) were used set up a colorimetric sandwich-type lateral flow immunoassay (LFIA). Combinations including one or two mAbs, either as capture detection reagent, explored...
Simultaneous measurement of different substances from a single sample is an emerging issue to achieve efficient and high-throughput detection in several fields application. Although immunoanalytical techniques have well-established prevailing advantages over alternative screening analytical platforms, one the incoming challenges for immunoassay exactly multiplexing. The Lateral Flow Immunoassay (LFIA) leading technique onsite analysis thanks its simplicity, rapidity cost-effectiveness....
A molecularly imprinted sorbent assays for cortisol was optimized direct determination in human saliva.
Direct pen writing offers versatile opportunities for development of low-cost tests point-of-care applications. In this work a lateral flow immunoassay (LFIA) test was fabricated by hand "writing" immunoprobes onto hand-cut nitrocellulose strips with commercial fountain pen. The qualitative capabilities the were extended addition Raman reporter and consequent design fabrication Surface Enhanced Resonant Scattering (SERRS)-LFIA test. As proof-of-concept, dual detection penicillin G achieved...