A5090156285- Animal Virus Infections Studies
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Malaria Research and Control
- Vector-Borne Animal Diseases
- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Vector-borne infectious diseases
- Rabies epidemiology and control
- SARS-CoV-2 detection and testing
- Hepatitis Viruses Studies and Epidemiology
- Poxvirus research and outbreaks
- Zoonotic diseases and public health
- Respiratory viral infections research
- Bat Biology and Ecology Studies
- Plant Virus Research Studies
- Cytomegalovirus and herpesvirus research
- Viral Infections and Outbreaks Research
- Microbial infections and disease research
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2016-2025
National Influenza Center
2024
Institute for Medical Research
2023
Universiti Teknologi MARA System
2023
Ospedaliera di Piacenza
2023
Ferrari (Italy)
2023
Lombardia Informatica (Italy)
2023
Regione Lombardia
2023
University of Parma
2023
Schüßler-Plan (Germany)
2023
Background In recent years human diseases due to mosquito-borne viruses were increasingly reported in Emilia-Romagna region (Italy), from the chikungunya virus 2007 West Nile (WNV) 2008. An extensive entomological survey was performed 2009 establish presence and distribution of mosquito arboviruses this region, with particular reference flaviviruses. Methodology/Principal Findings From May 6 October 31, a total 190,516 mosquitoes sampled georeferenced stations, grouped 1,789 pools according...
Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of severe acute respiratory syndrome (SARS) and Middle East (MERS) CoV in humans. This study describes identification molecular characterization alphacoronaviruses betacoronaviruses bats Italy, from 2010 to 2012. Sixty-nine faecal samples 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs detected seven nine carcasses. A phylogenetic analysis...
In April 2023, an outbreak of clade 2.3.4.4b highly pathogenic avian influenza A(H5N1) viruses carrying the T271A mammalian adaptive mutation in PB2 protein was detected a backyard poultry farm Italy. Five domestic dogs and one cat living on premises had seroconverted absence clinical signs. Virological serological monitoring individuals exposed to virus proved human transmission, however, asymptomatic infections pets may have important public health implications.
In August 2023, six locally acquired dengue virus 1 infections were detected in Lodi province, Lombardy Region, northern Italy, where the vector Aedes albopictus is present. Four cases hospitalised, none died. The viruses clustered with Peruvian and Brazilian strains collected between 2021 2023. This preliminary report highlights importance of continued integrated surveillance imported vector-borne potential for tropical disease outbreaks highly populated regions Italy competent vectors are
Middle East respiratory syndrome coronavirus (MERS-CoV), which belongs to beta group of coronavirus, can infect multiple host species and causes severe diseases in humans. Multiple surveillance phylogenetic studies suggest a bat origin. In this study, we describe the detection full genome characterization two CoVs closely related MERS-CoV from Italian bats, Pipistrellus kuhlii Hypsugo savii. Pool viscera were tested by pan-coronavirus RT-PCR. Virus isolation was attempted inoculation...
There have been previous reports of the human-to-cat transmission SARS-CoV-2, but there are only a few molecular studies that compared whole genome virus in cats and their owners. We here describe case domestic SARS-CoV-2 from healthcare worker to his cat for which nasopharyngeal swabs both its owner were used full-genome analysis. The results indicate quarantine measures should be extended pets living SARS-CoV-2-infected households.
Recently, Italy-particularly the Emilia-Romagna region-was location of consecutive outbreaks human diseases caused by arboviruses chikungunya virus and West Nile virus. The two outbreaks, spread different species mosquitoes, were not related, but pointed out lack an arboviral surveillance program in this region. Beginning 2007 entomological was initiated region, 2008 improved extended at Lombardia Using CO(2)-baited traps, 65,292 mosquitoes collected; pooled date collection, location,...
Summary This study describes the isolation and molecular characterization of Mammalian orthoreovirus (MRV) in microbats. Faecal samples dead individuals available from rehabilitation centres or collected known roost sites were virologically tested. In total, 112 carcasses bats found dead, 44 faecal analysed. Nineteen viral strains isolated by vitro cell culture tissue different bat species (Pipistrellus khulii, Tadarida teniotis, Rhinolophus hipposideros Vespertilio murinus), they...
Infectious bronchitis is considered to be one of the most devastating diseases in poultry. Control its spread typically attempted through biosecurity measures and extensive vaccination. However, remarkable genetic antigenic variability virus, which originate from both mutations recombination events, represents an unsolved challenge for this disease. The present study reports on emergence recombinant clusters detected Italy Spain between 2012 2014. A total 36 Spanish Italian infectious virus...
West Nile virus lineage 2 (WNV-2) was mainly confined to sub-Saharan Africa until the early 2000s, when it identified for first time in Central Europe causing outbreaks of human and animal infection. The aim this study reconstruct origin dispersion WNV-2 Italy on a phylodynamic phylogeographical basis. To aim, discrete continuous space models were applied total 33 newly characterised full-length viral genomes obtained from mosquitoes, birds humans Northern years 2013-2015 aligned with 64...
West Nile virus (WNV) is a recently re-emerged health problem in Europe. In Italy, an increasing number of outbreaks disease, with occurrences human cases, have been reported since 2008. This particularly true northern where entomological surveillance systems implemented at regional level. The aim this study was to use, for the first time, all data collected five regions undergoing WNV Italy characterize viral circulation (at spatial and temporal scale), identify potential mosquito vectors,...
Hypericin is a photosensitizing drug that active against membrane-enveloped viruses and therefore constitutes promising candidate for the treatment of SARS-CoV-2 infections. The antiviral efficacy hypericin largely determined by its affinity toward viral components number molecules loaded on single viruses. Here we use an experimental approach to follow interaction with SARS-CoV-2, evaluate efficacy, both in dark upon photoactivation. Binding particles directly visualized fluorescence...
Here we reported the virological, entomological and epidemiological characteristics of large autochthonous outbreak dengue (DENV) occurred in a small village Lombardy region (Northern Italy) during summer 2023.
The European subtype of tick-borne encephalitis virus (TBEV-Eur; species Orthoflavivirus encephalitidis, family Flaviviridae) was the only flavivirus present in central Europe known to cause neurologic disease humans and several animal species. Here, we report a isolated from Alpine chamois (Rupicapra rupicapra rupicapra) with attached ticks, over wide area Alps. Cases were detected 2017 Salzburg, Austria, 2023 Lombardy Piedmont, Italy. strains exhibit 94.8–97.3% nucleotide identities each...
A fatal Encephalomyocarditis virus (EMCV) infection epidemic involving fifteen primates occurred between October 2006 and February 2007 at the Natura Viva Zoo. This large open-field zoo park located near Lake Garda in Northern Italy hosts one thousand animals belonging to hundred fifty different species, including various lemur species. collection is most relevant rich Italy. second outbreak September November 2008 involved three lemurs. In all cases, clinical signs were sudden deaths...
Porcine enterovirus (PEV), Teschovirus and sapelovirus, belonging to the family Picornaviridae, are ubiquitous mainly cause asymptomatic infections in pigs. In this study, a total of 40 Italian porcine picornavirus isolates were characterized by sequencing capsid VP1-encoding gene. This procedure turned out be useful diagnostic tool for molecular identification enterovirus, teschovirus sapelovirus strains study epidemiology evolution these viruses confirming possibility correlating virus...
With several human cases reported annually since 2008 and the unapparent risk of infection blood donors, West Nile virus (WNV) is emerging as an important health issue in Europe. Italy, well other European countries, experienced a recrudescence circulation 2018, which led to increased number cases. An integrated surveillance plan was activated Emilia-Romagna Lombardy regions (Northern Italy) order monitor intensity timing WNV circulation. A fundamental part this consists entomological...
Lumpy skin disease (LSD) is an infectious affecting bovine with severe symptomatology. The implementation of effective control strategies to prevent infection outbreak requires rapid diagnostic tools. Two monoclonal antibodies (mAbs), targeting different epitopes the LSDV structural protein p32, and gold nanoparticles (AuNPs) were used set up a colorimetric sandwich-type lateral flow immunoassay (LFIA). Combinations including one or two mAbs, either as capture detection reagent, explored...
A renewed interest in mammalian orthoreoviruses (MRVs) has emerged since new viruses related to bat MRV type 3, detected Europe, were identified humans and pigs with gastroenteritis. This study reports the isolation characterization of a novel reassortant from lesser horseshoe (Rhinolophus hipposideros). The isolate, here designated BatMRV1-IT2011, was first by electron microscopy confirmed using PCR virus-neutralization tests. full genome sequence obtained next-generation sequencing....