Mark D. Stenglein
A5061136260- Viral Infections and Vectors
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- HIV Research and Treatment
- Animal Disease Management and Epidemiology
- Plant and Fungal Interactions Research
- Animal Virus Infections Studies
- Insect symbiosis and bacterial influences
- Microbial infections and disease research
- Parasites and Host Interactions
- Immune Cell Function and Interaction
- Virology and Viral Diseases
- Helminth infection and control
- Parasitic Diseases Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Herpesvirus Infections and Treatments
- Zoonotic diseases and public health
- Agriculture and Farm Safety
Colorado State University
2016-2025
Collins College
2021
University of California, San Francisco
2010-2015
University of Minnesota
2006-2013
Howard Hughes Medical Institute
2012
Masonic Cancer Center
2010
Institute of Molecular Biology and Biophysics
2008-2010
Institute of Virology of the Slovak Academy of Sciences
2008-2010
Sahlgrenska University Hospital
1999
The human APOBEC3 proteins are DNA cytidine deaminases that impede the replication of many different transposons and viruses. genes encode APOBEC3A, APOBEC3B, APOBEC3C, APOBEC3D, APOBEC3F, APOBEC3G APOBEC3H were generated through relatively recent recombination events. resulting high degree inter-relatedness has complicated development specific quantitative PCR assays for these despite considerable interest in understanding their expression profiles. Here, we describe a set specifically...
The most common transposable genetic element in humans, long interspersed 1 (L1), constitutes about 20% of the genome. activity L1 and related transposons such as Alu elements causes disease contributes to speciation. Little is known cellular mechanisms that control their spread. We show expression human APOBEC3B or APOBEC3F decreased rate retrotransposition by 5-10-fold. Expression two proteins, APOBEC3D APOBEC3G, had little effect. mechanism inhibition did not correlate with an obvious...
ABSTRACT Inclusion body disease (IBD) is an infectious fatal of snakes typified by behavioral abnormalities, wasting, and secondary infections. At a histopathological level, the identified presence large eosinophilic cytoplasmic inclusions in multiple tissues. To date, no virus or other pathogen has been definitively characterized associated with disease. Using metagenomic approach to search for candidate etiologic agents confirmed IBD, we de novo assembled complete genomic sequences two...
The mammalian APOBEC3 proteins are an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity that attacks nascent viral cDNA during reverse transcription, causing lethal mutagenesis. It has been suggested, but not formally shown, also induce sublethal mutagenesis, which would maintain contribute variation. To test this, we developed a...
Dengue virus is an emerging infectious agent that infects estimated 50-100 million people annually worldwide, yet current diagnostic practices cannot detect etiologic pathogen in ∼40% of dengue-like illnesses. Metagenomic approaches to detection, such as viral microarrays and deep sequencing, are promising tools address non-diagnosable disease challenges. In this study, we used the Virochip microarray sequencing characterize spectrum viruses present human sera from 123 Nicaraguan patients...
Anopheles gambiae are a major vector of malaria in sub-Saharan Africa. Viruses that naturally infect these mosquitoes may impact their physiology and ability to transmit pathogens. We therefore used metagenomics sequencing search for viruses adult collected from Liberia, Senegal, Burkina Faso. identified number virus virus-like sequences mosquito midgut contents, including 14 coding-complete genome segments 26 partial sequences. The define new the order Mononegavirales, families...
Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. There are currently no molecular clones of New World ZIKV available lack significant attenuation, hindering progress toward understanding determinants transmission and pathogenesis. Here we report the development characterization novel reverse genetics system based on 2015 isolate...
Significance SARS-CoV-2 emerged because of viral spillover from animals to humans, and spillback other animal species has been observed with accelerating frequency. Cross-species transmission generally results in the rapid adaptation virus new host, repeated transmissions may hasten evolution novel strain emergence. We report surprisingly selection numerous variants cell culture following infection nonhuman mammalian hosts, including dogs cats. These molecular changes provide insight into...
Members of the family Arenaviridae produce enveloped virions containing genomes consisting two or three single-stranded RNA segments totalling about 10.5 kb. Arenaviruses can infect mammals, including humans and other primates, snakes, fish. This is a summary International Committee on Taxonomy Viruses (ICTV) Report , which available at www.ictv.global/report/arenaviridae .
Arenaviridae is a family for ambisense RNA viruses with genomes of about 10.5 kb that infect mammals, snakes, and fish. The arenavirid genome consists two or three single-stranded segments encodes nucleoprotein (NP), glycoprotein (GP) large (L) protein containing RNA-directed polymerase (RdRP) domains; some arenavirids encode zinc-binding (Z). This summary the International Committee on Taxonomy Viruses (ICTV) report Arenaviridae, which available at www.ictv.global/report/arenaviridae.
Arenaviruses are one of the largest families human hemorrhagic fever viruses and known to infect both mammals snakes. package a large (L) small (S) genome segment in their virions. For segmented RNA like these, novel genotypes can be generated through mutation, recombination, reassortment. Although it is believed that an ancient recombination event led emergence new lineage mammalian arenaviruses, neither nor reassortment has been definitively documented natural arenavirus infections. Here,...
ABSTRACT A severe, sometimes fatal respiratory disease has been observed in captive ball pythons ( Python regius ) since the late 1990s. In order to better understand this and its etiology, we collected case control samples performed pathological diagnostic analyses. Electron micrographs revealed filamentous virus-like particles lung epithelial cells of sick animals. Diagnostic testing for known pathogens did not identify an etiologic agent, so unbiased metagenomic sequencing was performed....
Ixodes scapularis ticks harbor a variety of microorganisms, including eukaryotes, bacteria and viruses. Some these can be transmitted to cause disease in humans other vertebrates. Others are not pathogenic, but may impact the ability tick transmit pathogens. A growing number studies have examined influence on vector competence virome remains less clear, despite surge discovery tick-associated In this study, we performed shotgun RNA sequencing 112 individual adult I. collected Wisconsin, USA....
Partitiviruses are segmented, multipartite double-stranded RNA (dsRNA) viruses that until recently were only known to infect fungi, plants, and protozoans. Metagenomic surveys have revealed partitivirus-like sequences also commonly associated with arthropods. One arthropod-associated partitivirus, galbut virus, is common in wild populations of
Galbut virus (family Partitiviridae) infects Drosophila melanogaster and can be transmitted vertically from infected mothers or fathers with near perfect efficiency. This form of super-Mendelian inheritance should drive infection to 100% prevalence, indeed, galbut is ubiquitous in wild D. populations. However, on average, only about 60% individual flies are infected. One possible explanation for this that a subset resistant infection. Although virus-infected appear healthy, may sufficiently...