A5100716088- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Plant Virus Research Studies
- Viral Infections and Outbreaks Research
- Protein purification and stability
- Fire effects on ecosystems
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Transgenic Plants and Applications
- Respiratory viral infections research
- Fungal Plant Pathogen Control
- CRISPR and Genetic Engineering
- Influenza Virus Research Studies
- Ion channel regulation and function
- Esophageal and GI Pathology
Chinese Academy of Sciences
2016-2025
Wuhan Institute of Virology
2016-2025
East China University of Science and Technology
2025
Henan University of Science and Technology
2015-2025
The University of Texas MD Anderson Cancer Center
2023-2025
Institute of Forensic Science
2024
University of Chinese Academy of Sciences
2021-2024
China Agricultural University
2019-2024
Shanghai Electric (China)
2024
Institute of Microelectronics
2023-2024
Severe acute respiratory syndrome (SARS) emerged in 2002 to 2003 southern China. The origin of its etiological agent, the SARS coronavirus (SARS-CoV), remains elusive. Here we report that species bats are a natural host coronaviruses closely related those responsible for outbreak. These viruses, termed SARS-like (SL-CoVs), display greater genetic variation than SARS-CoV isolated from humans or civets. human and civet isolates nestle phylogenetically within spectrum SL-CoVs, indicating virus...
Dear Editor,To date, the number of confirmed coronavirus disease 2019 (COVID-19) cases has surpassed 100 million, with deaths exceeding 2 yet mechanism by which severe acute respiratory syndrome (SARS-CoV)-2 attacks body remains unclear.Although SARS-CoV-2 is known to primarily target lung, it also believed cause multi-organ dysfunction and comprehensive studies on cell tropism in humans are lacking.SARS-CoV-2 exploits host angiotensin-converting enzyme (ACE2) as its receptor for entry 1 ,...
The discovery of novel drug candidates with anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) potential is critical for the control global COVID-19 pandemic. Artemisinin, an old antimalarial derived from Chinese herbs, has saved millions lives. Artemisinins are a cluster artemisinin-related drugs developed treatment malaria and have been reported to multiple pharmacological activities, including anticancer, antiviral, immune modulation. Considering broad-spectrum antiviral...
The nucleotide sequence of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV) DNA genome was determined and analysed. circular encompasses 131403 bp, has a G+C content 39·1 mol% contains five homologous regions with unique pattern repeats. Computer-assisted analysis revealed 135 putative ORFs 150 nt or larger; 100 have homologues in Autographa californica multicapsid NPV (AcMNPV) further 15 other baculoviruses such as Lymantria dispar MNPV (LdMNPV), Spodoptera exigua...
ABSTRACT Baculoviruses produce two progeny phenotypes during their replication cycles. The occlusion-derived virus (ODV) is responsible for initiating primary infection in the larval midgut, and budded (BV) phenotype secondary infection. proteomics of several baculovirus ODVs have been revealed, but so far, no extensive analysis BV-associated proteins has conducted. In this study, protein composition BV Autographa californica nucleopolyhedrovirus (AcMNPV), type species baculoviruses, was...
Severe acute respiratory syndrome coronavirus (SARS-CoV) is a recently identified human coronavirus. The extremely high homology of the viral genomic sequences between viruses isolated from (huSARS-CoV) and those palm civet origin (pcSARS-CoV) suggested possible civet-to-human transmission. Genetic analysis revealed that spike (S) protein pcSARS-CoV huSARS-CoV was subjected to strongest positive selection pressure during transmission, there were six amino acid residues within...
Abstract Recent studies have suggested that bats are the natural reservoir of a range coronaviruses (CoVs), and rhinolophid harbor viruses closely related to severe acute respiratory syndrome (SARS) CoV, which caused an outbreak illness in humans during 2002–2003. We examined evolutionary relationships between bat CoVs their hosts by using sequence data virus RNA-dependent RNA polymerase gene cytochrome b gene. Phylogenetic analyses showed multiple incongruent associations phylogenies CoVs,...
The type I interferon (IFN) system, including IFN induction and signaling, is the critical component of host defense line against viral infection, which, in turn, also a vulnerable target for immune evasion. Severe fever with thrombocytopenia syndrome virus (SFTSV) an emerging bunyavirus. Previous data have shown that SFTSV can interfere early IFNs through targeting kinases TBK1/IKKε. In this study, we demonstrated suppress IFN-triggered signaling interferon-stimulated gene (ISG) expression....
Crimean–Congo hemorrhagic fever virus (CCHFV), a with high mortality in humans, is member of the genus Nairovirus family Bunyaviridae , and causative agent severe (HF). It classified as biosafety level 4 pathogen potential bioterrorism due to its aerosol infectivity ability cause HF outbreaks case fatality (∼30%). However, little known about structural features function nucleoproteins (NPs) especially CCHFV. Here we report 2.3-Å resolution crystal structure CCHFV nucleoprotein. The protein...
Tick-borne viral diseases have attracted much attention in recent years because of their increasing incidence and threat to human health. Severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) Heartland virus (HRTV) were recently identified as tick-borne phleboviruses (TBPVs) Asia the United States, respectively, are associated severe similar clinical manifestations. In this study, we report first identification isolation a novel TBPV named Guertu (GTV) from Dermacentor nuttalli...
For antiviral signaling mediated by retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), the recruitment of cytosolic RLRs and downstream molecules (such as TBK1 IKKε) to mitochondrial platform is a central event that facilitates establishment host state. Here, we present an example viral targeting for immune evasion through spatial isolation TBK1/IKKε from platform, which was employed severe fever with thrombocytopenia syndrome virus (SFTSV), deadly bunyavirus emerging recently. We...
Melanization, an important insect defense mechanism, is mediated by clip-domain serine protease (cSP) cascades and regulated serpins. Here we show that proteolytic activation of prophenoloxidase (PPO) PO-catalyzed melanization kill the baculovirus in vitro. Our quantitative proteomics biochemical experiments revealed infection cotton bollworm, Helicoverpa armigera, reduced levels most cascade members host hemolymph PO activity. By contrast, serpin-9 serpin-5 were sequentially upregulated...
Extensive research has been conducted on plant protection unmanned aerial vehicle (UAV) chemical application technology in recent years owing to its importance as a means of pest and disease control. UAV spraying orchards faces the drawback drift risk can be hazardous non-targeted crops, humans, environment. A detailed systematic analysis must performed determine uniformity sprays. In this study, peach orchard is sprayed with plant-protection at three different flight velocities we evaluate...
White spot syndrome virus (WSSV) is at present one of the major pathogens in shrimp culture worldwide. The complete genome this has been sequenced recently. To identify structural and functional proteins WSSV, purified virions were separated by SDS-PAGE. Twenty-four protein bands excised, in-gel digested with trypsin, subjected to matrix-assisted laser desorption ionization-time flight mass spectrometry electrospray ionization tandem spectrometry, respectively. Eighteen matching open reading...
The complete nucleotide sequence of Helicoverpa zea single-nucleocapsid nucleopolyhedrovirus (HzSNPV) has been determined (130869 bp) and compared to the armigera (Ha) SNPV. These two genomes are very similar in their (97% identity) amino acid (99% sequences. coding regions much more conserved than non-coding regions. In HzSNPV/HaSNPV, 63 open reading frames (ORFs) present all baculoviruses sequenced so far other ORFs. HzSNPV four additional small ORFs with HaSNPV, one these (Hz42) being a...
ABSTRACT The replication of lepidopteran baculoviruses is characterized by the production two progeny phenotypes: occlusion-derived virus (ODV), which establishes infection in midgut cells, and budded (BV), disseminates to different tissues within a susceptible host. To understand structural, hence functional, differences between BV ODV, we employed multiple proteomic methods reveal protein compositions posttranslational modifications phenotypes Helicoverpa armigera nucleopolyhedrovirus. In...
ABSTRACT Five highly conserved per os infectivity factors, PIF1, PIF2, PIF3, PIF4, and P74, have been reported to be essential for oral of baculovirus occlusion-derived virus (ODV) in insect larvae. Three these proteins, were thought function binding midgut cells. In this paper evidence is provided that PIF3 form a stable complex on the surface ODV particles Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV). The could withstand 2% SDS-5% β-mercaptoethanol with heating at...