A5073913486- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Optical Network Technologies
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- Photonic and Optical Devices
- Respiratory viral infections research
- Advanced Photonic Communication Systems
- Cytomegalovirus and herpesvirus research
- Animal Disease Management and Epidemiology
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- interferon and immune responses
- RNA regulation and disease
- Immune Response and Inflammation
- Insect Pest Control Strategies
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
National Institutes for Food and Drug Control
2024
Beijing University of Technology
2024
Institute of Pharmacology
2020
Shanghai Jiao Tong University
2018-2019
Wuhan Institute of Virology
2013-2014
Institute of Microbiology
2009-2013
There is currently an outbreak of respiratory disease caused by a novel coronavirus. The virus has been named severe acute syndrome coronavirus 2 (SARS-CoV-2) and the it causes 2019 (COVID-19). More than 16% patients developed distress syndrome, fatality ratio was 1%–2%. No specific treatment reported. Herein, we examined effects favipiravir (FPV) versus lopinavir (LPV)/ritonavir (RTV) for COVID-19. Patients with laboratory-confirmed COVID-19 who received oral FPV (Day 1: 1600 mg twice...
Abstract The COVID-19 pandemic has emerged as a global health emergency due to its association with severe pneumonia and relative high mortality. However, the molecular characteristics pathological features underlying remain largely unknown. To characterize mechanisms pathogenesis in lung tissue using proteomic approach, fresh tissues were obtained from newly deceased patients pneumonia. After virus inactivation, quantitative approach combined bioinformatics analysis was used detect changes...
The discovery of novel drug candidates with anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) potential is critical for the control global COVID-19 pandemic. Artemisinin, an old antimalarial derived from Chinese herbs, has saved millions lives. Artemisinins are a cluster artemisinin-related drugs developed treatment malaria and have been reported to multiple pharmacological activities, including anticancer, antiviral, immune modulation. Considering broad-spectrum antiviral...
The development of vaccines against infectious diseases represents one the most important contributions to medical science. However, vaccine-preventable still cause millions deaths each year due thermal instability and poor efficacy vaccines. Using human enterovirus type 71 vaccine strain as a model, we suggest combined, rational design approach improve thermostability immunogenicity live by self-biomineralization. biomimetic nucleating peptides are rationally integrated onto capsid reverse...
Mode-division multiplexing on an integrated photonic chip is critical for future optical networks. High-order mode desired to increase the transmission capacity. Here, we propose and experimentally demonstrate a silicon on-chip high-order (de)multiplexer using subwavelength grating (SWG) structure, which supports 11-mode (de)multiplexing TE-polarized light (TE <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> -TE...
Japanese encephalitis (JE) remains the leading cause of viral in Asia-Pacific region, and live vaccine SA14-14-2 is currently recommended by WHO widely used Asian countries with a good safety efficacy profile. In this study, we demonstrated that failed to produce NS1’, larger NS1-related protein, compared its parental strain SA14 various cells. Sequence analysis secondary structure prediction identified single silent mutation G66A NS2A-coding region destabilized conserved pseudoknot...
It is known that lethal viruses profoundly manipulate host metabolism, but how the metabolism alternation affects immediate antiviral immunity remains elusive. Here, we report O-GlcNAcylation of mitochondrial antiviral-signaling protein (MAVS), a key mediator interferon signaling, critical regulation to activate innate against RNA viruses. We show depletion in myeloid cells renders more susceptible virus infection both vitro and vivo. Mechanistically, demonstrate MAVS required for...
Class I enveloped viruses share similarities in their apparent use of a hexameric coiled-coil assembly to drive the merging virus and host cell membranes. Inhibition coiled coil-mediated interactions using bioactive peptides that replicate an α-helical chain from viral fusion machinery has significant antiviral potential. Here, we present construction series lipopeptides composed de novo heptad repeat sequence-based peptide plus hydrocarbon tail. Promisingly, constructs adopted stable...
BackgroundThe pharmacokinetics and appropriate dose regimens of favipiravir are unknown in hospitalized influenza patients; such data also needed to determine dosage selection for trials COVID-19.MethodsIn this dose-escalating study, tolerability were assessed critically ill patients. Participants received one two dosing regimens; Japan licensed (1600 mg BID on day 1 600 the following days) higher (1800 mg/800 BID) trialed uncomplicated influenza. The primary pharmacokinetic endpoint was...
Abstract Localized scleroderma (LoS) is a rare chronic disease with extensive tissue fibrosis, inflammatory infiltration, microvascular alterations, and epidermal appendage lesions. However, deeper understanding of the pathogenesis treatment strategies LoS currently limited. In present work, proteome map skin established, pathological features are characterized. Most importantly, human‐induced pluripotent stem cell‐derived epithelial mesenchymal (EM) organoids model in 3D culture system for...
The DENV-NS5 RNA-dependent RNA polymerase (RdRp) is essential for viral replication, and one of the targets anti-virus. In this study, Uni-VSW module was used to virtual screen 1.6 million compounds in ChemDiv TargetMol (USA) database, 27 candidates were obtained. Thereby 23 selected based on their binding free energies by 50 ns MD simulations. biological activity reference (BCX4430 Compound 27) evaluated IC50 values against DENV-NGC, CC50 values, selectivity index. Among these, D1 D8 13.06...
The EV-A71 poses a serious threat to the health and lives of children. can be transmitted by direct indirect skin contact. Therefore, there is an urgent need create novel models using human-derived cells study biology pathogenesis virus facilitate drug screening. Here, we use human induced pluripotent stem cells-derived organoids (hiPSC-SOs) as model for infection find that multiple cell types within organoids, including epidermal cells, hair follicle fibroblasts, nerve express receptors are...
Abstract Background Human enterovirus 71 (EV71) has emerged as a significant cause of acute encephalitis and deaths in young children. The clinical manifestations caused by EV71 varied from mild hand, foot mouth disease to severe neurological complications deaths, but its pathogenesis remains elusive. Antibody dependent enhancement (ADE) infection been reported various viruses shown contribute severity. Results In this study, the presence sub-neutralizing antibody was demonstrated enhance...
The emerging human enterovirus 71 (EV71) represents a growing threat to public health, and no vaccine or specific antiviral is currently available. Human intravenous immunoglobulin (IVIG) clinical used in treating severe EV71 infections. However, the discovery of antibody dependent enhancement (ADE) infection illustrates complex roles controlling infection. In this study, identify distinct role each IgG subclass on neutralization infection, different lots pharmaceutical IVIG preparations...
Flaviviruses including Zika virus, Dengue Japanese Encephalitis and Yellow Fever virus cause heavy burdens to public health around the world. No specific antiviral drug is available in clinic against these flavivirus infections. Heat-shock protein 70 (HSP70) has recently been proven be a promising target virus. Here, we report that Apoptozole, small molecule inhibitor of ATPase activity HSP70, broad-spectrum anti-flavivirus potential. The mode action analysis revealed Apoptozole acted at...
Abstract Zika virus (ZIKV) infection can be the cause of congenital malformations, including microcephaly in infants and other disorders such as Guillain-Barré syndrome, meningoencephalitis, myelitis, which also occur some infected adults. However, at this time, there is no drug approved to treat ZIKV infection. Drug repurposing promptest way obtain an effective during a global public health emergency spread virus. In study, we report US Food Admistration-approved that safe for pediatric...
The recent outbreak of novel coronavirus pneumonia (COVID-19) caused by a new has posed great threat to public health. Identifying safe and effective antivirals is urgent demand cure the huge number patients. Virus-encoded proteases are considered potential drug targets. human immunodeficiency virus protease inhibitors (lopinavir/ritonavir) been recommended in global Solidarity Trial March launched World Health Organization. However, there currently no experimental evidence support or...