A5024835172- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Analytical chemistry methods development
- Alzheimer's disease research and treatments
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Bacteriophages and microbial interactions
- Estrogen and related hormone effects
- Enzyme Production and Characterization
- Nuclear Receptors and Signaling
- Analytical Chemistry and Chromatography
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Sperm and Testicular Function
- Neuroscience and Neuropharmacology Research
- Bacterial Genetics and Biotechnology
- ATP Synthase and ATPases Research
- Medicinal Plant Pharmacodynamics Research
- RNA modifications and cancer
- Cholinesterase and Neurodegenerative Diseases
- Pharmacological Effects and Assays
Zhejiang University
2015-2025
Southeast University
2024
Zhongda Hospital Southeast University
2024
Zhejiang Cancer Hospital
2024
Ruijin Hospital
2022-2023
Shanghai Jiao Tong University
2017-2023
First Affiliated Hospital Zhejiang University
2019-2023
Chinese PLA General Hospital
2009-2020
Institute of Botany
2020
Chinese Academy of Sciences
2020
Neurofibrillary tangles composed of hyperphosphorylated, aggregated tau are a common pathological feature tauopathies, including Alzheimer's disease. Abnormal phosphorylation by kinases or phosphatases has been proposed as pathogenic mechanism in tangle formation. To investigate whether kinase inhibition can reduce tauopathy and the degeneration associated with it vivo , transgenic mice overexpressing mutant human were treated glycogen synthase kinase-3 (GSK-3) inhibitor lithium chloride....
Metabolic Sensor The enzyme O-GlcNAc transferase (OGT) catalyzes the transfer of N -acetylglucosamine from uridine diphospho- (UDP-GlcNAc) to serine or threonine residues intracellular proteins and responds metabolic status cell. Yi et al. (p. 975 ; see Perspective by Mattaini Vander Heiden ) show that O-GlcNAcylation phosphofructokinase 1 (PFK1) reduces its activity, thus influencing rates glycolysis within cells. PFK1 was increased in cells exposed hypoxia, several cell lines derived human...
Estrogen receptors (ERs) are believed to be ligand-activated transcription factors belonging the nuclear receptor superfamily, which on ligand binding translocate into nucleus and activate gene transcription. To date, two ERs have been identified: ERα ERβ. plays major role in estrogen-mediated genomic actions both reproductive nonreproductive tissue, whereas function of ERβ is still unclear. In this study, we used immunocytochemistry, immunoblotting, proteomics demonstrate that localizes...
Postoperative cognitive dysfunction, confusion, and delirium are common after general anesthesia in the elderly, with symptoms persisting for months or years some patients. Even middle-aged patients likely to have postoperative dysfunction surgery, Alzheimer's disease (AD) appear be particularly at risk of deterioration anesthesia. Several investigators thus examined whether is associated AD, studies suggesting that exposure anesthetics may increase AD. However, little known on biochemical...
Abstract Many cancer cells display enhanced glycolysis and suppressed mitochondrial metabolism. This phenomenon, known as the Warburg effect, is critical for tumor development. However, how coordinate glucose metabolism through tricarboxylic acid (TCA) cycle largely unknown. We demonstrate here that phosphoglycerate kinase 1 (PGK1), first ATP-producing enzyme in glycolysis, reversibly dynamically modified with O-linked N-acetylglucosamine (O-GlcNAc) at threonine 255 (T255). O-GlcNAcylation...
Neuroprotective strategies, including free radical scavengers, ion channel modulators, and anti-inflammatory agents, have been extensively explored in the last 2 decades for treatment of neurological diseases. Unfortunately, none neuroprotectants has proved effective clinical trails. In current study, we demonstrated that methylene blue (MB) functions as an alternative electron carrier, which accepts electrons from NADH transfers them to cytochrome c bypasses complex I/III blockage. A de...
Abstract The pentose phosphate pathway (PPP) plays a critical role in macromolecule biosynthesis and maintaining cellular redox homoeostasis rapidly proliferating cells. Upregulation of the PPP has been shown several types cancer. However, how is regulated to confer selective growth advantage on cancer cells not well understood. Here we show that glucose-6-phosphate dehydrogenase (G6PD), rate-limiting enzyme PPP, dynamically modified with an O-linked β-N-acetylglucosamine sugar response...
Programmed-death ligand 1 (PD-L1) and its receptor programmed cell death (PD-1) mediate T cell-dependent immunity against tumors. The abundance of surface PD-L1 is a key determinant the efficacy immune checkpoint blockade therapy targeting PD-L1. However, regulation still poorly understood. Here, we show that lysosomal degradation regulated by O-linked N-acetylglucosamine (O-GlcNAc) during intracellular trafficking pathway. O-GlcNAc modifies hepatocyte growth factor-regulated tyrosine kinase...
Cyclin-dependent kinase 5 (cdk5) and glycogen synthase 3β (GSK3β) have been implicated in pathogenic processes associated with Alzheimer's disease because both kinases regulate tau hyperphosphorylation enhance amyloid precursor protein (APP) processing leading to an increase β (Aβ) production. Here we show that young p25 overexpressing mice enhanced cdk5 activity but reduced GSK3β attributable phosphorylation at the inhibitory GSK3β–serine 9 (GSK3β–S9) site. Phosphorylation this site was...
Methylene blue (MB), the first lead chemical structure of phenothiazine and other derivatives, is commonly used in diagnostic procedures as a treatment for methemoglobinemia. We have previously demonstrated that MB could function an alternative mitochondrial electron transfer carrier, enhance cellular oxygen consumption, provide protection vitro rodent models Parkinson’s disease stroke. In present study, we investigated structure-activity relationships using six structurally related...
Abstract The aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor that responds to environmental toxicants, is increasingly recognized as key player in embryogenesis and tumorigenesis. Here we show variety of tryptophan derivatives act endogenous AhR ligands can affect the level master pluripotency Oct4. Among them, ITE enhances binding promoter Oct4 suppresses its transcription. Reduction levels cancer cells by deprivation or hypoxia leads elevation, which be reverted...
Ogt catalyzed O-linked N-acetylglucosamine (O-GlcNAcylation, O-GlcNAc) plays an important function in diverse biological processes and diseases. However, the roles of regulating neurogenesis remain largely unknown. Here, we show that deficiency or depletion adult neural stem/progenitor cells (aNSPCs) leads to diminishment aNSPC pool aberrant consequently impairs cognitive mice. RNA sequencing reveals alters transcription genes relating cell cycle, neurogenesis, neuronal development....
Significance Epidermal growth factor receptor (EGFR) is one of the most important membrane receptors that transduce signals into cells to sustain cell growth, proliferation, and survival. EGFR signal termination initiated by internalization, followed trafficking through endosomes, degradation in lysosomes. How this process regulated still poorly understood. Here, we show hepatocyte tyrosine kinase substrate (HGS), a key protein pathway, dynamically modified single sugar N-acetylglucosamine....
Aberrant mitosis occurs in many tauopathy-related neurodegenerative diseases and is believed to precede the formation of neurofibrillary tangles. In this study, we report for first time that transient cerebral ischemia induces aberrant mitotic proteins hyperphosphorylation tau protein with tangle-like conformational epitopes adult female rat cortex. Following rats, initiation apoptosis precedes potentially integrated subsequent hyperphosphorylation. Furthermore, inhibition mitosis-related...
Neisseria meningitidis PglL belongs to a novel family of bacterial oligosaccharyltransferases (OTases) responsible for O-glycosylation type IV pilins. Although members this are widespread among pathogenic bacteria, there is little known about their mechanism. Understanding the process may uncover potential targets therapeutic intervention, and can open new avenues exploitation these pathways biotechnological purposes. In work, we demonstrate that able transfer virtually any glycan from...