A5030193058- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Hydrogen's biological and therapeutic effects
- Autophagy in Disease and Therapy
- Renal and related cancers
- Sulfur Compounds in Biology
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cardiac Ischemia and Reperfusion
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Carcinogens and Genotoxicity Assessment
- Nanoparticle-Based Drug Delivery
- Pancreatic function and diabetes
- Plant Molecular Biology Research
- Biochemical effects in animals
- Cancer, Hypoxia, and Metabolism
- Sirtuins and Resveratrol in Medicine
- Plant Reproductive Biology
- Aldose Reductase and Taurine
- Cytokine Signaling Pathways and Interactions
- Zebrafish Biomedical Research Applications
- Curcumin's Biomedical Applications
Zhejiang University
2016-2025
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2015-2025
Samsung Medical Center
2024
National Clinical Research Center for Digestive Diseases
2022
Korea University Medical Center
2020-2022
First Affiliated Hospital Zhejiang University
2020
Korea Institute of Science and Technology
2014-2016
Second Military Medical University
2011-2016
Korea Institute of Brain Science
2014-2016
Tibet Autonomous Region People's Hospital
2016
Root hair growth requires intensive cell-wall modification. This study demonstrates that root hair-specific expansin As, a sub-clade of the cell wall-loosening proteins, are required for elongation in rice (Oryza sativa L.). We identified gene encoding EXPA17 (OsEXPA17) from mutant with short hairs. Promoter::reporter transgenic lines exhibited exclusive OsEXPA17 expression cells. The protein (OsexpA17) contained point mutation, causing change amino acid sequence (Gly104→Arg). alteration is...
Abstract Background Autophagy plays a significant role in myocardial ischemia-reperfusion (IR) injury. So it is important to inhibit autophagy protect cardiomyocytes besides anti-apoptosis. MiRNA has been demonstrated against apoptosis during IR, while whether anti-autophagy effect not known. The aim of this study was investigate miR-204 regulated by regulating LC3-II protein, which the marker autophagosome IR Methods Adult SD rats were randomized Control and groups. group treated with 30...
Alzheimer's disease (AD) is a lethal progressive neurological disorder affecting the memory. Recently, US Food and Drug Administration mitigated standard for drug approval, allowing symptomatic drugs that only improve cognitive deficits to be allowed accelerate on clinical trials. Our study focuses taurine, an endogenous amino acid found in high concentrations humans. It has demonstrated neuroprotective properties against many forms of dementia. In this study, we assessed cognitively...
Abstract The aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor that responds to environmental toxicants, is increasingly recognized as key player in embryogenesis and tumorigenesis. Here we show variety of tryptophan derivatives act endogenous AhR ligands can affect the level master pluripotency Oct4. Among them, ITE enhances binding promoter Oct4 suppresses its transcription. Reduction levels cancer cells by deprivation or hypoxia leads elevation, which be reverted...
Background:Myxomas are the most common primary heart tumors and closely associated with embolic events.Cardiac myxomas typically arise from interatrial septum at border of fossa ovalis in left atrium.Any other location is considered atypical.Embolism, one complications myxoma, high morbidity mortality.The aim this study was to investigate risk factors for embolism patients cardiac myxoma. Material/Methods:In retrospective study, a cohort 162 surgically treated between January 1998 June 2014...
Background: Autophagy plays a significant role in myocardial ischemia reperfusion (IR) injury. Hydrogen sulfide (H2S) has been demonstrated to protect cardiomyocytes against IR injury, while whether it anti-autophagy effect not known. The aim of this study was investigate H2S regulates autophagy during injury and its possible mechanism. Methods Results: neonatal rats were randomized into Con, hypoxia-reoxygenation (HR) protection groups. severity cell evaluated by vitality (MTT) lactate...
Auxin plays a pivotal role in many facets of plant development. It acts by inducing the interaction between auxin-responsive [auxin (AUX)/indole-3-acetic acid (IAA)] proteins and ubiquitin protein ligase SCF(TIR) to promote degradation AUX/IAA proteins. Other cofactors chaperones that participate auxin signaling remain be identified. Here, we characterized rice (Oryza sativa) plants with mutations cyclophilin gene (OsCYP2). cyp2 mutants showed defects responses exhibited variety...
Abstract High-grade glioma is a highly malignant and metastatic brain cancer, resistant to many existing anticancer treatments. In such cancer cells, the glucose-regulated protein 78 kDa (GRP78) particularly up-regulated. Former studies have thus targeted mutation-free GRP78 not only detect cells specifically but also enhance cytotoxic effect. We focus on cell surface-expressed as target for suppressing high-grade lines. Glioblastoma multiforme (GBM) line, was first injected into 5-week-old...
Abstract The molecular mechanisms of aluminum (Al) toxicity and tolerance in plants have been the focus ongoing research area stress phytophysiology. Recent studies described Al-induced apoptosis-like cell death plant animal cells. In this study, we show that yeast (Saccharomyces cerevisiae) exposed to low effective concentrations Al for short times undergoes enhanced division a manner is dose density dependent. At higher or longer exposure times, induces growth inhibition. Several apoptotic...
Oct4 protein encoded by POU5F1 plays a pivotal role in maintaining the self‑renewal of pluripotent stem cells; however, its presence cancer cells remains controversial. In present study, we provided evidence that transcripts authentic OCT4 gene (OCT4A) and multiple pseudogenes were detected variety cell lines. A few major bands also western blotting using an anti‑Oct4A monoclonal antibody. Moreover, anti‑Oct4‑pT235 antibody was used to identify band majority tested lines coincided with one...
Abstract Despite substantial advances in treating cervical cancer (CC) with surgery, radiation and chemotherapy, patients advanced CC still have poor prognosis significantly variable clinical outcomes due to tumor recurrence metastasis. Therefore, develop more efficacious specific treatments for remains an unmet need. In this study, by virtual screening the SPECS database, we identified multiple novel JAK inhibitor candidates validated their antitumor drug efficacies that were particularly...
Extra-domain B of fibronectin (EDB-FN) is an alternatively spliced form with high expression in the extracellular matrix neovascularized tissues and malignant cancer cells.In this study, we evaluated practicality using EDB-FN as a biomarker therapeutic target for gliomas (MGs), representative intractable diseases involving brain tumors.Methods: The microarray-and sequence-based patient transcriptomic database 'Oncopression' tissue microarray MG samples were analyzed.EDB-FN data extracted...
Abstract Naive human embryonic stem cells (hESCs) that resemble the pre-implantation epiblasts are fueled by a combination of aerobic glycolysis and oxidative phosphorylation, but their mitochondrial regulators poorly understood. Here we report that, proline dehydrogenase (PRODH), mitochondria-localized metabolism enzyme, is dramatically upregulated in naive hESCs compared to primed counterparts. The upregulation PRODH induced reduction c-Myc expression dependent on PD0325901, MEK inhibitor...
Hydrogen sulfide (H<Sub>2</Sub>S) is a powerful inhibitor of cardiomyocytes apoptosis following ischemia/reperfusion (IR) injury, but the underlying mechanism remains unclear. Our previous study showed that microRNA-1 (miR-1) was upregulated by 2.21 fold in IR group compared with H<Sub>2</Sub>S preconditioned group. MiR-206 affected process hypertrophy regulating histone deacetylase 4 (HDAC4). HDAC4 also known to play an anti-apoptotic role tumor cells, its myocardium has not been reported....
Abstract AKT serves as an epigenetic modulator that links regulation to cell survival and proliferation while the mediator OCT4 critically controls stem pluripotency self-renewal. Emerging evidence indicated their complicated interplays in cancer cells (CSCs), inhibiting either one may activate other. Thus, this study, we propose a strategy targeting both factors simultaneously. Firstly, combination of OCT4-specific shRNA specific inhibitor Akti-1/2 potently suppressed propagation human...
Abstract OCT4A is well established as a master transcription factor for pluripotent stem cell (PSC) self-renewal and pioneer initiating somatic reprogramming, yet its presence functionality in cancer cells remain controversial obscure. By combining the CRISPR-Cas9-based gene editing with highly specific PCR assays, sensitive immunoassays, mass spectrometry, we provide unequivocal evidence here that full-length authentic transcripts proteins were both present cells, heterogeneously expressed...
Abstract Glioblastoma is the most malignant cancer in brain and currently incurable. It urgent to identify effective targets for this lethal disease. Inhibition of such should suppress growth cells and, ideally also precancerous early prevention, but minimally affect their normal counterparts. Using genetic mouse models with neural stem (NSCs) or oligodendrocyte precursor (OPCs) as cells‐of‐origin/mutation, it shown that susceptibility within development hierarchy glioma knockout...
This study aimed to explore the protective effect of hydrogen as an antioxidant on monocrotaline (MCT)-induced pulmonary hypertension (PH). Forty-eight SD rats were equally randomized into four groups: SHAM group, MCT MCT+Oral-H2 group and MCT+Inj-H2 group. The results showed that mean arterial pressure, right ventricle weight ventricular hypertrophy index in significant higher than those group; inflammatory response, atrial natriuretic factor, 3-nitrityrosine intercellular adhesion...
Autophagy is significant in myocardial ischemia‑reperfusion (IR) injury. Ulinastatin has been demonstrated to protect cardiomyocytes against IR through inducing anti‑inflammatory effects. However, whether ulinastatin an anti‑autophagic effect yet be elucidated. The present study aimed investigate the of on regulation autophagy during Cardiomyocytes neonatal rats were randomly divided into control, hypoxia‑reoxygenation (HR) and groups. In order mammalian target rapamycin (mTOR) involved...