A5006284972- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
- Plant responses to water stress
- Soybean genetics and cultivation
- Light effects on plants
- Plant Gene Expression Analysis
- Aluminum toxicity and tolerance in plants and animals
- Seed Germination and Physiology
- Antioxidant Activity and Oxidative Stress
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Agricultural Productivity and Crop Improvement
- Bioenergy crop production and management
- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Cell death mechanisms and regulation
- Biofuel production and bioconversion
- Circular RNAs in diseases
- Molecular Biology Techniques and Applications
- Plant Taxonomy and Phylogenetics
Zhejiang University
2016-2025
State Key Laboratory of Plant Physiology and Biochemistry
2005-2014
• MicroRNA (miRNA)-mediated regulation of auxin signaling components plays a critical role in plant development. miRNA expression and functional diversity contribute to the complexity regulatory networks miRNA/target modules. This study functionally characterizes two members rice (Oryza sativa) miR393 family their target genes, OsTIR1 OsAFB2 (AUXIN SIGNALING F-BOX), closest homologs Arabidopsis TRANSPORT INHIBITOR RESPONSE 1 (TIR1). We found that possess distinctive patterns, with miR393a...
Abstract Hydrogen sulfide (H 2 S) is an important gaseous molecule in various plant developmental processes and stress responses. In this study, the transgenic Arabidopsis thaliana plants with modulated expressions of two cysteine desulfhydrases, exogenous H S donor (sodium hydrosulfide, NaHS) scavenger (hypotaurine, HT) pre‐treated were used to dissect involvement The desulfhydrases overexpressing NaHS exhibited higher endogenous level improved abiotic tolerance biotic resistance, while...
Among the three primary auxin-induced gene families, Auxin/Indole-3-Acetic Acid (Aux/IAA), Gretchen Hagen3 (GH3) and SMALL AUXIN UP RNA (SAUR), function of SAUR genes remains unclear. Arabidopsis have been phylogenetically classified into clades. Recent work has suggested that SAUR19 (clade II) SAUR63 I) promote cell expansion through modulation auxin transport. Herein, we present our on SAUR41, a clade III with distinctive expression pattern in root meristems. SAUR41 was normally expressed...
miR396 targets seven GROWTH-REGULATING FACTOR (GRF) genes and the BASIC HELIX–LOOP–HELIX (bHLH) TRANSCRIPTION 74 gene (bHLH74) in Arabidopsis. Previous research revealed that target module regulates cell proliferation plays a critical role leaf development. However, no additional biological functions of have been investigated detail. In this study, T-DNA insertion mutants transgenic plants with altered levels or its were used to characterize regulatory root We found AtMIR396a was predominant...
High-throughput small RNA sequencing has identified several potential aluminum (Al)-responsive microRNAs (miRNAs); however, their regulatory role remains unknown. Here, we two miR393 family members in barley, and confirmed target genes—HvTIR1 HvAFB—through a modified form of 5′-RACE (rapid amplification cDNA ends) as well degradome data analysis. Furthermore, investigated the biological function miR393/target module root development its Al stress response. The investigation showed that...
Abstract Background and Aims Most primary auxin response genes are classified into three families: AUX/IAA, GH3 SAUR genes. Few studies have been conducted on Arabidopsis thaliana genes, possibly due to genetic redundancy among different subfamily members. Data mining arabidopsis transcriptional profiles indicates that the SAUR41 members of SMALL AUXIN UP RNA are, strikingly, induced by an inhibitory phytohormone, abscisic acid (ABA). We aimed reveal physiological roles containing SAUR40,...
Auxin regulates almost every aspect of plant growth and development is perceived by the TIR1/AFB auxin co-receptor proteins differentially acting in concert with specific Aux/IAA transcriptional repressors. Little known about diverse functions family members species other than Arabidopsis. We created targeted OsTIR1 OsAFB2-5 mutations rice using CRISPR/Cas9 genome editing, functionally characterized roles these five auxinic herbicide resistance. Our results demonstrated that OsTIR1/AFB are...
Detached Arabidopsis thaliana leaves can regenerate adventitious roots, providing a platform for studying de novo root regeneration (DNRR). However, the comprehensive transcriptional framework of DNRR remains elusive. Here, we provide high-resolution landscape transcriptome reprogramming from wound response to organogenesis in and show key factors involved DNRR. Time-lapse RNA sequencing (RNA-seq) entire leaf within 12 h detachment revealed rapid activation jasmonate, ethylene, reactive...
Abstract MicroRNAs (miRNAs) play fundamental roles in many developmental and physiological processes eukaryotes. MiRNAs plants generally regulate their targets via either mRNA cleavage or translation repression; however, which approach plays a major role whether these two function modes can shift remains elusive. Here, we identify miRNA, miR408-5p that regulates AUXIN/INDOLE ACETIC ACID 30 ( IAA30 ), critical repressor the auxin pathway switching action rice. We find usually inhibits protein...
Gloxinia (Sinningia speciosa) is a popular commercial plant for its attractive and colourful flowers. However, the genetic mechanism of flowering time regulation in gloxinia largely unknown. Recent studies on model plants have elucidated that miR159 acts as negative regulator floral transition short-day photoperiods. The aim this study was to investigate whether modification expression can offer an effective approach characteristics gloxinia.Transgenic were generated over-express or suppress...
The conserved miRNA393 family is thought to be involved in root elongation, leaf development and stress responses, but its role during seed germination seedling establishment remains unclear. In this study, expression of the MIR393a/target module germinating rice (Oryza sativa L.) seeds were investigated. β-Glucuronidase (GUS) analysis showed that MIR393a OsTIR1 had spatial-temporal transcriptional activities radicle roots, coleoptile tips stomata cells, corresponding a dynamic auxin...
Vitamin E (tocochromanol) is a lipid-soluble antioxidant and an essential nutrient for human health. Among cereal crops, barley (Hordeum vulgare) contains high level of vitamin E, which includes both tocopherols tocotrienols. Although the biosynthetic pathway has been characterized in dicots, such as Arabidopsis, only accumulate tocopherols, knowledge regarding biosynthesis monocots limited because lack functional mutants. This study aimed to obtain gene knockout mutants elucidate genetic...
Small RNA and degradome sequencing have identified a large number of miRNA-target pairs in plant seeds. However, detailed spatial temporal studies miRNA-mediated regulation, which can reflect links between seed development germination are still lacking.In this study, we extended our investigation on miRNAs-involved gene regulation by combined analysis maturation barley. Through bioinformatics small data, total 1324 known miRNA families 448 novel candidates were identified. Of those, 16...
Abstract Background Gene duplication is a prevalent phenomenon and major driving force underlying genome evolution. The process leading to the fixation of gene duplicates following critical understand how evolves but remains fragmentally understood. Most previous studies on retention are based duplicate analyses in single reference genome. No population-based comparative analysis has been performed date. Results Taking advantage recently published genomic data Triticeae , we dissected...
Abstract The molecular mechanisms of aluminum (Al) toxicity and tolerance in plants have been the focus ongoing research area stress phytophysiology. Recent studies described Al-induced apoptosis-like cell death plant animal cells. In this study, we show that yeast (Saccharomyces cerevisiae) exposed to low effective concentrations Al for short times undergoes enhanced division a manner is dose density dependent. At higher or longer exposure times, induces growth inhibition. Several apoptotic...
Bamboo (Phyllostachys praecox) is one of the largest members grass family Poaceae, and most economically important crops in Asia. However, complete knowledge bamboo development its molecular mechanisms still lacking. In present study, differences anatomical structure among rhizome buds, shoots, shoots were compared, several genes related to bud identified. The rice cross-species microarray hybridization showed a total 318 up-regulated 339 down-regulated genes, including those involved...
Vacuolar processing enzymes (VPEs) are cysteine proteinases that act as crucial mediators of programmed cell death (PCD) in plants. In rice, however, the role VPEs abiotic stress-induced PCD remains largely unknown. this study, we generated OsVPE3 overexpression and suppression transgenic lines to elucidate function gene rice.Survival rate chlorophyll retention analyses showed clearly enhanced salt stress tolerance rice compared with wild type. Furthermore, fragmentation genomic DNA was...
Auxin is an important phytohormone in plants, and auxin signaling pathways rice play key roles regulating its growth, development, productivity. To investigate how grain yield traits are regulated by to facilitate their application improvement, we validated the functional relationships among regulatory genes such as OsIAA10, OsSK41, OsARF21 that involved one of (OsIAA10) pathways. We assessed phenotypic effects these on several across two environments using knockout and/or overexpression...
Hydrogen peroxide (H(2)O(2)) is known to be a key player in apoptosis animals. The components and pathways regulating H(2)O(2)-induced programmed cell death plants, however, remain largely unknown. In the present study, rice transgenic lines overexpressing Bcl-2, human apoptotic suppressor, were obtained. These showed increased tolerance high levels of H(2)O(2), resulting seed germination rates, root elongation, tip viability chlorophyll retention compared control lines. lines, treatment...