A5084824722- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- Bioenergy crop production and management
- Autophagy in Disease and Therapy
- Wheat and Barley Genetics and Pathology
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Plant biochemistry and biosynthesis
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Rice Cultivation and Yield Improvement
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Antioxidant Activity and Oxidative Stress
- Genetic Mapping and Diversity in Plants and Animals
- Cocoa and Sweet Potato Agronomy
- Antimicrobial Peptides and Activities
- Graphene and Nanomaterials Applications
- Microbial Natural Products and Biosynthesis
- Polysaccharides and Plant Cell Walls
Hangzhou Normal University
2021-2024
Zhejiang University
2011-2024
Zhejiang Center for Disease Control and Prevention
2022
Australian Centre for Plant Functional Genomics
2015-2017
The University of Adelaide
2015-2017
State Key Laboratory of Food Science and Technology
2016
Nanchang University
2016
State Key Laboratory of Plant Physiology and Biochemistry
2011
• MicroRNA (miRNA)-mediated regulation of auxin signaling components plays a critical role in plant development. miRNA expression and functional diversity contribute to the complexity regulatory networks miRNA/target modules. This study functionally characterizes two members rice (Oryza sativa) miR393 family their target genes, OsTIR1 OsAFB2 (AUXIN SIGNALING F-BOX), closest homologs Arabidopsis TRANSPORT INHIBITOR RESPONSE 1 (TIR1). We found that possess distinctive patterns, with miR393a...
High-throughput small RNA sequencing has identified several potential aluminum (Al)-responsive microRNAs (miRNAs); however, their regulatory role remains unknown. Here, we two miR393 family members in barley, and confirmed target genes—HvTIR1 HvAFB—through a modified form of 5′-RACE (rapid amplification cDNA ends) as well degradome data analysis. Furthermore, investigated the biological function miR393/target module root development its Al stress response. The investigation showed that...
This paper outlines a numerical scheme for accurate, detailed, and high-throughput image analysis of plant roots. In contrast to existing root tools that focus on system-average traits, novel, fully automated robust approach the detailed characterization based graph optimization process is presented. The scheme, firstly, distinguishes primary roots from lateral and, secondly, quantifies broad spectrum traits each identified root. Thirdly, it associates their properties with specific which...
Vitamin E (tocochromanol) is a lipid-soluble antioxidant and an essential nutrient for human health. Among cereal crops, barley (Hordeum vulgare) contains high level of vitamin E, which includes both tocopherols tocotrienols. Although the biosynthetic pathway has been characterized in dicots, such as Arabidopsis, only accumulate tocopherols, knowledge regarding biosynthesis monocots limited because lack functional mutants. This study aimed to obtain gene knockout mutants elucidate genetic...
Abstract Background Gene duplication is a prevalent phenomenon and major driving force underlying genome evolution. The process leading to the fixation of gene duplicates following critical understand how evolves but remains fragmentally understood. Most previous studies on retention are based duplicate analyses in single reference genome. No population-based comparative analysis has been performed date. Results Taking advantage recently published genomic data Triticeae , we dissected...
Vacuolar processing enzymes (VPEs) are cysteine proteinases that act as crucial mediators of programmed cell death (PCD) in plants. In rice, however, the role VPEs abiotic stress-induced PCD remains largely unknown. this study, we generated OsVPE3 overexpression and suppression transgenic lines to elucidate function gene rice.Survival rate chlorophyll retention analyses showed clearly enhanced salt stress tolerance rice compared with wild type. Furthermore, fragmentation genomic DNA was...
The genus Citrus is characterized by a reticulate evolutionary history with frequent hybridization, making it an intriguing subject for genome evolution investigation. changshan Y. B. Chang (Huyou) unique landrace first discovered in Zhejiang Province, China premium fruit quality. origin of Huyou has puzzled local botanists and growers. Here, we sequenced 120-years-old "ancestral tree" using PacBio long read Hi-C sequencing assembled 2 high-quality haplotype-resolved genomes HY1 HY2....
Hydrogen peroxide (H(2)O(2)) is known to be a key player in apoptosis animals. The components and pathways regulating H(2)O(2)-induced programmed cell death plants, however, remain largely unknown. In the present study, rice transgenic lines overexpressing Bcl-2, human apoptotic suppressor, were obtained. These showed increased tolerance high levels of H(2)O(2), resulting seed germination rates, root elongation, tip viability chlorophyll retention compared control lines. lines, treatment...
Abstract Background Barley is known to be recalcitrant tissue culture, which hinders genetic transformation and its biotechnological application. To date, the ideal explant for remains limited immature embryos; mechanism underlying embryonic callus formation elusive. Results This study aimed uncover different transcription regulation pathways between calli formed from (IME) mature (ME) embryos through transcriptome sequencing. We showed that incubation of in an auxin-rich medium caused...
Barley has abundant anthocyanin-rich accessions, which renders it an ideal model to investigate the regulatory mechanism of anthocyanin biosynthesis. This study functionally characterized two transcription factors: Ant1 and Ant2. Sequence alignment showed that coding sequences Ant2 are conserved among 11 colored hulless barley noncolored varieties. The expression profiles were divergent between species, significantly higher was found in Qingke accessions. co-expression resulted purple...
Plant growth-promoting rhizobacteria (PGPR) are well-acknowledged root endophytic bacteria used for plant growth promotion. However, which metabolites produced by PGPR could promote remains unclear. Additionally, genes responsible traits is also not elucidated. Thus, as comprehensive understanding of the mechanism endophyte in promotion limited, this study aimed to determine and involved growth-promotion. We isolated an Rhizobium sp. WYJ-E13 strain from roots Curcuma wenyujin Y.H. Chen et C....
Tocotrienols and tocopherols (vitamin E) are potent antioxidants that synthesized in green plants. Unlike ubiquitous tocopherols, tocotrienols predominantly accumulate the endosperm of monocot grains, catalyzed by homogentiate geranylgeranyl transferase (HGGT). Previously, we generated a tocotrienol-deficient hvhggt mutant with shrunken barley grains. However, relationship between grain development remains unclear. Here, found lines displayed hollow endosperms defective transfer cells...
Homogentisate phytyltransferase (HPT) is the critical enzyme for biosynthesis of tocopherols (vitamin E), which are major lipid-soluble antioxidants and help plants adapt to various stress conditions. HPT generally strictly conserved in plant genomes; however, a divergent lineage HPT2 was identified recently some Triticeae species. The molecular function transcriptional profiles remain be characterized.In this study, we performed comprehensive transcriptome data mining HPT1 different tissues...
Autophagy is essential to maintain cellular homeostasis for normal cell growth and development. In selective autophagy, ATG8 plays a crucial role in cargo target recognition by binding various adaptors receptors with the ATG8-interacting motif, also known as LC3-interacting region (LIR). However, process of autophagy callus, proliferating type, largely unknown. this study, we overexpressed green fluorescent protein (GFP)-ATG8a GFP-ATG8b transgenic barley callus checked their autophagic...
Antioxidant of bamboo leaves (AOB) was certified to be a natural antioxidant by the Chinese Ministry Health in 2003. However, effects AOB on animal reproductive and developmental functions remain unclear. The present study aimed investigate different concentrations mouse embryonic fibroblast (MEF) cells, examine underlying molecular mechanism through which affects proliferation apoptosis MEFs. MEFs prepared from individual embryos were treated with various dosages AOB. Cell viability...
Zearalenone (ZEN) is a secondary metabolite from Fusarium species. It also present in plants and regulates the photochemical reaction Photosystem II, stress response root growth. To investigate mechanism by which ZEN Tetrastigma hemsleyanum growth, differentially expressed microRNAs (miRNAs) were identified verified high-throughput sequencing Agrobacterium rhizogenes-mediated transformation of roots T. seedlings treated with without ZEN. The predicted functions microRNA156b (miR156b)...
Abstract Background: Barley is known to be recalcitrant tissue culture, which hinders genetic transformation and its biotechnological application. To date, the ideal explant for still limited immature embryos; however, mechanism underlying embryonic callus formation remains elusive. Results: The aim of this study was uncover differential transcription regulation pathways between embryo (IME)- mature (ME)-derived through transcriptome sequencing. We showed that incubation embryos on...