A5001322572- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Plant Stress Responses and Tolerance
- Biofuel production and bioconversion
- Microbial Community Ecology and Physiology
- RNA Research and Splicing
- Light effects on plants
- Legume Nitrogen Fixing Symbiosis
- Plant and Fungal Interactions Research
- Plant Gene Expression Analysis
- Microbial bioremediation and biosurfactants
- Cancer-related molecular mechanisms research
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
Zhejiang University
2016-2025
Institute of Crop Sciences
2012-2025
Sanya University
2024-2025
Nanjing Forestry University
2025
Institute of Atmospheric Physics
2015-2024
Chinese Academy of Sciences
2010-2024
Ministry of Agriculture and Rural Affairs
2024
Hainan University
2023-2024
China National Rice Research Institute
2017-2023
Second Military Medical University
2023
Pathogen-associated molecular patterns (PAMPs) trigger plant defenses when perceived by surface-localized immune receptors. PAMP-triggered immunity (PTI) plays a vital role in the resistance of plants to numerous potential pathogens. MicroRNA (miRNA) biogenesis is known be important for PTI, but miRNA species involved this process have not been fully explored. Here we show that Arabidopsis (Arabidopsis thaliana) effector protein, Argonaute1 (AGO1), required number PTI responses including...
MicroRNAs (miRNAs) are small silencing RNAs with regulatory roles in gene expression. miRNAs interact Argonaute (AGO) proteins to form effector complexes that cleave target mRNAs or repress translation. Rice (Oryza sativa) encodes four AGO1 homologs (AGO1a, AGO1b, AGO1c, and AGO1d). We used RNA interference (RNAi) knock down the AGO1s. The RNAi lines displayed pleiotropic developmental phenotypes had increased accumulation of miRNA targets. AGO1a, AGO1c were purified further characterized....
Nascent allohexaploid wheat may represent the initial genetic state of common (Triticum aestivum), which arose as a hybrid between Triticum turgidum (AABB) and Aegilops tauschii (DD) by chromosome doubling outcompeted its parents in growth vigor adaptability. To better understand molecular basis for this success, we performed mRNA small RNA transcriptome analyses nascent following generations, their progenitors, natural cultivar Chinese Spring, with assistance recently published A D genome...
MicroRNAs (miRNAs) are indispensable regulators for development and defense in eukaryotes. However, the miRNA species have not been explored rice (Oryza sativa) immunity against blast fungus Magnaporthe oryzae, most devastating fungal pathogen production worldwide. Here, by deep sequencing small RNA libraries from susceptible resistant lines normal conditions upon M. oryzae infection, we identified a group of known miRNAs that were differentially expressed infection. They further classified...
Abstract Trans-acting small interfering RNAs (ta-siRNAs; TAS) emerge as a class of plant-specific that are initiated from microRNA-mediated cleavage TAS gene transcripts. It has been revealed ta-siRNAs generated by the sequential activities SUPPRESSOR OF GENE SILENCING3 (SGS3), RNA-DEPENDENT RNA POLYMERASE6 (RDR6), and DICER-LIKE4 (DCL4), loaded into ARGONAUTE1 (AGO1) proteins to posttranscriptionally regulate several target genes messenger in trans. Here, we showed high cytosine DNA...
Organ abscission is a key step in plant's life cycle and one of the most important agronomic traits for crops. In tomato, two MADS-box genes, JOINTLESS (J) MACROCAYLYX (MC), have been shown to be implicated development flower zone (AZ), but molecular mechanisms underlying this process are not well known. We report here that SEPALLATA (SEP) gene SLMBP21 acts as an additional factor AZ tomato. show knockdown abolishes AZ, while overexpression produces small cells at proximal section pedicel...
MicroRNAs play crucial roles in plant responses to pathogen infections. The rice blast disease, caused by the fungus Magnaporthe oryzae, is most important disease of (Oryza sativa). To explore microRNA species that participate immunity against we compared expression small RNAs between mock- and M. oryzae-treated rice. We found infection oryzae strain Guy11 specifically induced miR319 and, consequently, suppressed its target gene TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTOR1...
The contradiction between “high yielding” and “early maturing” hampers further improvement of annual rice yield. Here we report the positional cloning a major maturity duration regulatory gene, Early flowering-completely dominant ( Ef-cd ), demonstrate that natural variation in could be used to overcome above contradictory. locus gives rise long noncoding RNA (lncRNA) antisense transcript overlapping OsSOC1 gene. lncRNA expression positively correlates with H3K36me3 deposition. Field test...
Abstract MicroRNAs (miRNAs) play fundamental roles in many developmental and physiological processes eukaryotes. MiRNAs plants generally regulate their targets via either mRNA cleavage or translation repression; however, which approach plays a major role whether these two function modes can shift remains elusive. Here, we identify miRNA, miR408-5p that regulates AUXIN/INDOLE ACETIC ACID 30 ( IAA30 ), critical repressor the auxin pathway switching action rice. We find usually inhibits protein...
The highly conserved florigen gene FLOWERING LOCUS T (FT) functions at the core of flowering pathways. Extensive studies have examined transcriptional regulation FT; however, other layers FT remain unclear. Here, we identified miR5200 a Pooideae-specific microRNA that is expressed in leaves and targets Brachypodium distachyon orthologs for mRNA cleavage. was abundantly plants grown under short-day (SD) conditions but dramatically repressed transferred to long-day (LD) conditions. We also...
ABSTRACT The biological and regulatory roles of photosensory proteins are poorly understood for nonphotosynthetic bacteria. foliar bacterial pathogen Pseudomonas syringae has three protein-encoding genes that predicted to encode the blue-light-sensing LOV (light, oxygen, or voltage) histidine kinase (LOV-HK) two red/far-red-light-sensing bacteriophytochromes, BphP1 BphP2. We provide evidence LOV-HK form an integrated network regulates swarming motility in response multiple light wavelengths....
Abstract Appropriate flowering timing is crucial for plant reproductive success. The florigen, FLOWERING LOCUS T (FT), interacts with 14-3-3 proteins and the bZIP transcription factor FD, functioning at core nodes in multiple pathways. There are two FT homologues, FT1 FT2, Brachypodium distachyon . Here we show that FT2 undergoes age-dependent alternative splicing (AS), resulting splice variants ( FT2α FT2β ). -encoded protein cannot interact FD or 14-3-3s but able to form heterodimers FT1,...
Summary DNA methylation is dynamically involved in plant immunity, but little information known about its roles interactions with biotrophic fungi, especially temperate grasses such as wheat ( Triticum aestivum ). Using diploid progenitor Aegilops tauschii accession AL 8/78, the genome of which has been sequenced, we assessed extent response to infection Blumeria graminis f. sp. tritici Bgt ), causes powdery mildew. Upon infection, ARGONAUTE 4a AGO ) was significantly downregulated A. ,...
Abstract Timing of reproductive transition is precisely modulated by environmental cues in flowering plants. Facultative long-day plants, including Arabidopsis and temperate grasses, trigger rapid conditions (LDs) delay under short-day (SDs). Here, we characterize a SD-induced FLOWERING LOCUS T ortholog, FT-like 9 (FTL9), that promotes SDs but inhibits LDs Brachypodium distachyon . Mechanistically, like photoperiod-inductive FT1, FTL9 can interact with FD1 to form activation complex (FAC),...
Tomato flower abscises at the anatomically distinct abscission zone that separates pedicel into basal and apical portions. During abscission, cell separation occurs only indicating distinctive molecular regulation in its cells. We conducted a transcriptome analysis of tomato tissues during ethylene promoted abscission. found was most active site with largest set differentially expressed genes when compared Gene Ontology analyses revealed enriched transcription hydrolase activities zone. also...