A5087051199- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Micronutrient Interactions and Effects
- Plant Reproductive Biology
- Genetics and Plant Breeding
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Crop Yield and Soil Fertility
- Bioenergy crop production and management
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant Genetic and Mutation Studies
- Plant Pathogens and Resistance
- Legume Nitrogen Fixing Symbiosis
- Agricultural Productivity and Crop Improvement
- Protein Kinase Regulation and GTPase Signaling
- Plant responses to elevated CO2
- CRISPR and Genetic Engineering
Institute of Crop Sciences
2015-2024
Chinese Academy of Agricultural Sciences
2015-2024
Xiamen University
2021
Ministry of Agriculture and Rural Affairs
2020
Institute of Crop Science
2013-2020
Harbin Veterinary Research Institute
2018
State Key Laboratory of Plant Cell and Chromosome Engineering
2011
State Key Laboratory of Plant Genomics
2011
Institute of Genetics and Developmental Biology
2011
Chinese Academy of Sciences
2011
Sequencing and analysing the diploid genome transcriptome of Aegilops tauschii provide new insights into role this in enabling adaptation bread wheat are a step towards understanding very large complicated hexaploid genomes species. The Triticum aestivum, designated AABBDD, evolved as result hybridization between three ancestral grasses. Two papers published issue Nature present sequences analysis two these progenitors. First, sequence wild T. urartu (ancestor A genome), which resembles...
Activation of the mitogen-activated protein kinase (MAP kinase) isoforms ERK1 and ERK2 was investigated in rat adipocytes. Kinase activities were measured by using myelin basic as substrate after resolved Mono Q chromatography or immunoprecipitation with specific antibodies. Insulin increased activity both 3- to 4-fold. The beta-adrenergic agonist isoproterenol without effect absence insulin but markedly reduced increases produced hormone. MAP activation also attenuated forskolin glucagon,...
Resistance May Not Be Futile Recently, Ug99, a particularly devastating strain of wheat stem rust fungus, has emerged, which could potentially threaten food security. Now, two genes have been cloned that offer resistance to Ug99. Saintenac et al. (p. 783 , published online 27 June) Sr35 from Triticum monococcum diploid species not often cultivated. Periyannan 786 Sr33 Aegilops tauschii wild grass contributed the hexaploid genome cultivated wheat. The both encode proteins show features...
Wheat is an important global crop with extremely large and complex genome that contains more transposable elements (TEs) than any other known species. Here, we generated a chromosome-scale, high-quality reference of Aegilops tauschii, the donor wheat D genome, in which 92.5% sequences have been anchored to chromosomes. Using this assembly, accurately characterized genic loci, gene expression, pseudogenes, methylation, recombination ratios, microRNAs especially TEs on In addition discovery...
Incubating rat aortic smooth muscle cells with either platelet-derived growth factor BB (PDGF) or insulin-like I (IGF-I) increased the phosphorylation of PHAS-I, an inhibitor mRNA cap binding protein, eukaryotic initiation (eIF) 4E. Phosphorylation PHAS-I promoted dissociation PHAS-I-eIF-4E complex, effect that could partly explain stimulation protein synthesis by two factors. Increasing cAMP forskolin decreased and markedly amount eIF-4E bound to effects consistent action inhibit synthesis....
The proteins of the MYB superfamily play central roles in developmental processes and defence responses plants. Sixty unique wheat genes that contain full-length cDNA sequences were isolated. These 60 grouped into three categories, namely one R1R2R3-MYB, 22 R2R3-MYBs, 37 MYB-related members. sequence composition R2 R3 repeats was conserved among R2R3-MYB proteins. Phylogenetic comparison members this wheat, rice, Arabidopsis revealed putative functions some clustered functional clades....
The MYB-type proteins are involved in various processes of plant growth, development, and stress response. In a previous work, polyethylene glycol (PEG) stress-induced gene, TaMYB30, which encodes R2R3-type MYB protein was identified wheat. this study, the isolation functional characterization TaMYB30 gene reported. Three homologous sequences were isolated from hexaploid wheat designated as TaMYB30-A, TaMYB30-B, TaMYB30-D genes based on localizations these three to chromosomes 2A, 2B, 2D,...
The basic region/leucine zipper (bZIP) transcription factors (TFs) play vital roles in the response to abiotic stress. However, little is known about function of bZIP genes wheat In this study, we report isolation and functional characterization TabZIP60 gene. Three homologous genome sequences were isolated from hexaploid mapped homoeologous group 6. A subcellular localization analysis indicated that a nuclear-localized protein activates transcription. Furthermore, gene transcripts strongly...
Abstract Background Rapid advances in next-generation sequencing methods have provided new opportunities for transcriptome (RNA-Seq). The unprecedented depth by RNA-Seq makes it a powerful and cost-efficient method study, has been widely used model organisms non-model to identify quantify RNA. For lacking well-defined genomes, de novo assembly is typically required downstream analyses, including SNP discovery identification of genes differentially expressed phenotypes. Although successfully...
NAC transcription factors play diverse roles in plant development and responses to abiotic stresses. However, the biological of family members wheat are not well understood. Here, we reported isolation functional characterization a novel TaNAC47 gene. encoded protein, localizing nucleus, is able bind ABRE cis-element transactivate yeast, suggesting that it likely functions as transcriptional activator. We also showed differentially expressed different tissues, its expression was induced by...
Early reproductive development in cereals is crucial for final grain number per spike and hence the yield potential of crop. To date, however, no systematic analyses gene expression profiles during this important process have been conducted common wheat (Triticum aestivum). Here, we studied transcriptome at four stages early development, from spikelet initiation to floral organ differentiation. K-means clustering stage-specific transcript identification detected dynamically expressed...
As one of the most important staple foods, wheat plays a crucial role in sustaining food security. However, its production is greatly threatened by abiotic stresses such as salt (Khataar et al., 2018; Qi 2019). Therefore, discovering genes involved stress tolerance and cultivating genetically modified plant varieties with enhanced are currently among goals for breeders (Genc To identify transcriptional factor (TF) that essential regulating tolerance, 1455 TF-encoding from full-length cDNA...
Abstract Wheat ( Triticum aestivum L.) is a major staple food for more than one-third of the world’s population. Tiller number an important agronomic trait in wheat, but only few related genes have been cloned. Here, we isolate wheat mutant, tiller number1 tn1 ), with much fewer tillers. We clone TN1 gene via map-based cloning: encodes ankyrin repeat protein transmembrane domain (ANK-TM). show that single amino acid substitution third conserved causes decreased mutant plants. Resequencing...
Abstract The utilization of stabilized DELLA proteins Rht-B1b and Rht-D1b was crucial for increasing wheat (Triticum aestivum) productivity during the Green Revolution. However, underlying mechanisms remain to be clarified. Here, we cloned a gain-of-function allele GSK3/SHAGGY-like kinase-encoding gene GSK3 by characterizing dwarf mutant. Furthermore, determined that interacts with phosphorylates Revolution protein promote it reduce plant height in wheat. Specifically, phosphorylation may...
Although the actions of insulin and a number growth factors that signal via protein-tyrosine kinase receptors are believed to involve increased phosphorylation key intracellular proteins, relatively few downstream phosphoproteins have been identified. In this report we describe cDNA encoding one most prominent insulin-stimulated in rat adipocytes. The encodes protein, designated PHAS-I, which has 117 amino acids M(r) 12,400. When translated vitro subjected SDS/PAGE, PHAS-I migrates...
Abstract Bread wheat (Triticum aestivum) is an allohexaploid species, consisting of three subgenomes (A, B, and D). To study the molecular evolution these closely related genomes, we compared sequence a 307-kb physical contig covering high weight (HMW)-glutenin locus from A genome durum turgidum, AABB) with orthologous regions B same D diploid Aegilops tauschii (Anderson et al., 2003; Kong 2004). Although gene colinearity appears to be retained, four out six genes including two paralogous...
Abstract Dominance, semidominance, and recessiveness are important modes of Mendelian inheritance. The phytohormone gibberellin (GA) regulates many plant growth developmental processes. previously cloned semidominant GA-insensitive (GAI) genes Reduced height1 (Rht1) Rht2 in wheat (Triticum aestivum) were the basis Green Revolution. However, no completely dominant GAI gene has been cloned. Here, we report molecular characterization Rht-B1c, a allele that confers more extreme characteristics...
• Rht-D1c (Rht10) carried by Chinese wheat (Triticum aestivum) line Aibian 1 is an allele at the Rht-D1 locus. Among Rht-1 alleles, little known about although it determines extreme dwarf phenotype in wheat. Here, we cloned and functionally characterized using a combination of Southern blotting, target region sequencing, gene expression analysis transgenic experiments. We found that was generated through tandem segmental duplication (TSD) > Mb region, resulting two copies Rht-D1b. Two...
Abstract The male-sterile ms2 mutant has been known for 40 years and become extremely important in the commercial production of wheat. However, gene responsible this phenotype remained unknown. Here we report map-based cloning Ms2 gene. locus is remarkable several ways that have implications basic biology. Beyond having no functional annotation, barely detectable transcription fertile wild-type wheat plants, accumulated destructive mutations orthologs, allele acquired a terminal-repeat...