Beat Keller
A5074186291- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant pathogens and resistance mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Chromosomal and Genetic Variations
- Genetics and Plant Breeding
- Plant Pathogens and Resistance
- Powdery Mildew Fungal Diseases
- Plant tissue culture and regeneration
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Plant Reproductive Biology
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Yeasts and Rust Fungi Studies
- Plant Pathogenic Bacteria Studies
- Agriculture, Plant Science, Crop Management
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
- Agriculture and Biological Studies
- Crop Yield and Soil Fertility
- Plant Genetic and Mutation Studies
University of Zurich
2016-2025
University Medical Center Freiburg
2025
University of Freiburg
2025
Zürcher Fachhochschule
2023
Australian National University
2023
Prince Philip Hospital
2023
Louisiana State University
2023
International Rice Research Institute
2023
University of Science and Technology
2023
Max Planck Institute for Plant Breeding Research
2006-2022
Sorghum, an African grass related to sugar cane and maize, is grown for food, feed, fibre fuel. We present initial analysis of the ∼730-megabase Sorghum bicolor (L.) Moench genome, placing ∼98% genes in their chromosomal context using whole-genome shotgun sequence validated by genetic, physical syntenic information. Genetic recombination largely confined about one-third sorghum genome with gene order density similar those rice. Retrotransposon accumulation recombinationally recalcitrant...
Insights from the annotated wheat genome Wheat is one of major sources food for much world. However, because bread wheat's a large hybrid mix three separate subgenomes, it has been difficult to produce high-quality reference sequence. Using recent advances in sequencing, International Genome Sequencing Consortium presents an with detailed analysis gene content among subgenomes and structural organization all chromosomes. Examples quantitative trait mapping CRISPR-based modification show...
An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across homeologous chromosomes and subgenomes. Comparative analysis subgenomes extant diploid tetraploid relatives showed that high similarity structural conservation are retained, with limited loss, after polyploidization. However, genomes there was evidence dynamic gain,...
Agricultural crops benefit from resistance to pathogens that endures over years and generations of both pest crop. Durable disease resistance, which may be partial or complete, can controlled by several genes. Some the most devastating fungal in wheat are leaf rust, stripe powdery mildew. The gene Lr34 has supported these for more than 50 years. is now shared cultivars around world. Here, we show LR34 protein resembles adenosine triphosphate-binding cassette transporters pleiotropic drug...
The coordinated expression of highly related homoeologous genes in polyploid species underlies the phenotypes many world's major crops. Here we combine extensive gene datasets to produce a comprehensive, genome-wide analysis homoeolog patterns hexaploid bread wheat. Bias varies between tissues, with ~30% wheat homoeologs showing nonbalanced expression. We found asymmetries along chromosomes, largest inter-tissue, inter-cultivar, and coding sequence variation, most often located...
Abstract Advances in genomics have expedited the improvement of several agriculturally important crops but similar efforts wheat ( Triticum spp.) been more challenging. This is largely owing to size and complexity genome 1 , lack genome-assembly data for multiple lines 2,3 . Here we generated ten chromosome pseudomolecule five scaffold assemblies hexaploid explore genomic diversity among from global breeding programs. Comparative analysis revealed extensive structural rearrangements,...
Plant immune responses are triggered by pattern recognition receptors that detect conserved pathogen-associated molecular patterns (PAMPs) or resistance (R) proteins recognizing isolate-specific pathogen effectors. We show in barley, intracellular mildew A (MLA) R function the nucleus to confer against powdery fungus. Recognition of fungal avirulence A10 effector MLA10 induces nuclear associations between receptor and WRKY transcription factors. The identified act as repressors...
Sequencing and analysing the diploid genome transcriptome of Aegilops tauschii provide new insights into role this in enabling adaptation bread wheat are a step towards understanding very large complicated hexaploid genomes species. The Triticum aestivum, designated AABBDD, evolved as result hybridization between three ancestral grasses. Two papers published issue Nature present sequences analysis two these progenitors. First, sequence wild T. urartu (ancestor A genome), which resembles...
The allohexaploid bread wheat genome consists of three closely related subgenomes (A, B, and D), but a clear understanding their phylogenetic history has been lacking. We used assemblies five diploid relatives to analyze genome-wide samples gene trees, as well estimate evolutionary relatedness divergence times. show that the A B genomes diverged from common ancestor ~7 million years ago these gave rise D through homoploid hybrid speciation 1 2 later. Our findings imply present-day is product...
More than 50 leaf rust resistance ( Lr ) genes against the fungal pathogen Puccinia triticina have been identified in wheat gene pool, and a large number of them extensively used breeding. Of genes, all are known only from their phenotype and/or map position except for Lr21 , which was cloned recently. For many years, problems molecular work (1.6 × 10 bp), highly repetitive (80%), hexaploid bread Triticum aestivum L.) genome hampered map-based cloning. Here, we report isolation Lr10 by using...
Summary In wheat, race‐specific resistance to the fungal pathogen powdery mildew ( Blumeria graminis f. sp. tritici ) is controlled by Pm genes. There are 10 alleles conferring at Pm3 locus Pm3a Pm3j on chromosome 1AS of hexaploid bread wheat Triticum aestivum L.). The genome has a size 1.6 × bp and contains more than 80% repetitive sequences, making positional cloning difficult. Here, we demonstrate that combined analysis genomes from species with different ploidy levels can be exploited...
Allohexaploid bread wheat ( Triticum aestivum L.) provides approximately 20% of calories consumed by humans. Lack genome sequence for the three homeologous and highly similar genomes (A, B, D) has impeded expression analysis grain transcriptome. We used previously unknown information to analyze cell type–specific genes in developing identified distinct co-expression clusters reflecting spatiotemporal progression during endosperm development. observed no global but type– stage-dependent...
Identification of causal mutations in barley and wheat is hampered by their large genomes suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting sequencing mutant chromosomes, to identify induced comparison parental chromosomes. We apply MutChromSeq six mutants each the Eceriferum-q gene Pm2 genes. This unambiguously identified single candidate genes that were verified Sanger additional mutants. enables...
Significance Northern corn leaf blight (NCLB) is one of the most devastating fungal diseases maize. The Htn1 disease resistance gene confers quantitative field against NCLB isolates. Here we show that encodes a putative wall-associated receptor-like kinase (RLK). RLKs act as important components first tier plant innate immune system by perceiving pathogen- or host-derived elicitors on cell surface. are often associated with to nonadapted pathogens and component nonhost resistance. Our work...
Abstract To improve our understanding of the organization and evolution wheat (Triticum aestivum) genome, we sequenced annotated 13-Mb contigs (18.2 Mb) originating from different regions its largest chromosome, 3B (1 Gb), produced a 2x chromosome survey by shotgun Illumina/Solexa sequencing. All carried genes irrespective their chromosomal location. However, gene distribution was not random, with 75% them clustered into small islands containing three on average. A twofold increase density...
Thomas Wicker and colleagues report the whole-genome sequencing of four wheat powdery mildew (Blumeria graminis forma specialis tritici) isolates from different geographic regions. Their comparative genomic analysis provides insights into evolution mildews, which are obligate biotropic fungal pathogens. Wheat mildew, Blumeria tritici, is a devastating pathogen with poorly understood evolutionary history. Here we draft genome sequence resequencing three additional regions analyses barley...
Abstract Rye ( Secale cereale L.) is an exceptionally climate-resilient cereal crop, used extensively to produce improved wheat varieties via introgressive hybridization and possessing the entire repertoire of genes necessary enable hybrid breeding. allogamous only recently domesticated, thus giving cultivated ryes access a diverse exploitable wild gene pool. To further enhance agronomic potential rye, we produced chromosome-scale annotated assembly 7.9-gigabase rye genome validated its...
Using Roche/454 technology, we sequenced the chloroplast genomes of 12 Triticeae species, including bread wheat, barley and rye, as well diploid progenitors relatives wheat Triticum urartu, Aegilops speltoides Ae. tauschii. Two wild tetraploid taxa, cylindrica geniculata, were also included. Additionally, incorporated Einkorn boeoticum its domesticated form T. monococcum two Hordeum spontaneum (wild barley) genotypes. Chloroplast used for overall sequence comparison, phylogenetic analysis...
Disease resistance genes encoding nucleotide-binding and leucine-rich repeat (NLR) intracellular immune receptor proteins detect pathogens by the presence of pathogen effectors. Plant genomes typically contain hundreds NLR-encoding genes. The availability hexaploid wheat (Triticum aestivum) cultivar Chinese Spring reference genome allows a detailed study its NLR complement. However, low expression high intrafamily sequence homology hinder their accurate annotation. Here, we developed...