A5102010446- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Chromosomal and Genetic Variations
- Nematode management and characterization studies
- Plant Gene Expression Analysis
- Genetic and phenotypic traits in livestock
- Crop Yield and Soil Fertility
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Genetic diversity and population structure
- Rice Cultivation and Yield Improvement
- Photovoltaic System Optimization Techniques
- Higher Education and Teaching Methods
- Advances in Cucurbitaceae Research
- Plant Pathogens and Resistance
- Solar Radiation and Photovoltaics
- Photosynthetic Processes and Mechanisms
- Plant Micronutrient Interactions and Effects
- Energy Load and Power Forecasting
- Plant Virus Research Studies
- Sesame and Sesamin Research
Central South University
2024
Second Xiangya Hospital of Central South University
2024
Huazhong University of Science and Technology
2023
North China Electric Power University
2022-2023
North Carolina State University
2022
Texas A&M University
2021-2022
Institut thématique Génétique, génomique et bioinformatique
2021
Amarillo College
2020
Kansas State University
2011-2018
South Dakota State University
2012
Summary High‐density single nucleotide polymorphism ( SNP ) genotyping arrays are a powerful tool for studying genomic patterns of diversity, inferring ancestral relationships between individuals in populations and marker–trait associations mapping experiments. We developed array including about 90 000 gene‐associated s used it to characterize genetic variation allohexaploid allotetraploid wheat populations. The includes significant fraction common genome‐wide distributed that represented...
Domesticated crops experience strong human-mediated selection aimed at developing high-yielding varieties adapted to local conditions. To detect regions of the wheat genome subject during improvement, we developed a high-throughput array interrogate 9,000 gene-associated single-nucleotide polymorphisms (SNP) in worldwide sample 2,994 accessions hexaploid including landraces and modern cultivars. Using SNP-based diversity map characterized impact crop improvement on genomic geographic...
The allohexaploid bread wheat genome consists of three closely related subgenomes (A, B, and D), but a clear understanding their phylogenetic history has been lacking. We used assemblies five diploid relatives to analyze genome-wide samples gene trees, as well estimate evolutionary relatedness divergence times. show that the A B genomes diverged from common ancestor ~7 million years ago these gave rise D through homoploid hybrid speciation 1 2 later. Our findings imply present-day is product...
Allohexaploid bread wheat ( Triticum aestivum L.) provides approximately 20% of calories consumed by humans. Lack genome sequence for the three homeologous and highly similar genomes (A, B, D) has impeded expression analysis grain transcriptome. We used previously unknown information to analyze cell type–specific genes in developing identified distinct co-expression clusters reflecting spatiotemporal progression during endosperm development. observed no global but type– stage-dependent...
Bread wheat is an allopolyploid species with a large, highly repetitive genome. To investigate the impact of selection on variants distributed among homoeologous genomes and to build foundation for understanding genotype-phenotype relationships, we performed population-scale re-sequencing diverse panel lines.A sample 62 lines was re-sequenced using whole exome capture genotyping-by-sequencing approaches. We describe allele frequency, functional significance, chromosomal distribution 1.57...
Abstract The emergence of new sequencing technologies has provided fast and cost-efficient strategies for high-resolution mapping complex genomes. Although these approaches hold great promise to accelerate genome analysis, their application in studying genetic variation wheat been hindered by the complexity its polyploid genome. Here, we applied next-generation a doubled-haploid population gene tested utility ordering shotgun sequence contigs flow-sorted chromosome. A bioinformatical...
The high level of identity among duplicated homoeologous genomes in tetraploid pasta wheat presents substantial challenges for de novo transcriptome assembly. To solve this problem, we develop a specialized bioinformatics workflow that optimizes assembly and separation merged homoeologs. evaluate our strategy, sequence assemble the one diploid ancestors wheat, compare both assemblies with benchmark set 13,472 full-length, non-redundant bread cDNAs. A total 489 million 100 bp paired-end reads...
Abstract Cycles of whole-genome duplication (WGD) and diploidization are hallmarks eukaryotic genome evolution speciation. Polyploid wheat (Triticum aestivum) has had a massive increase in size largely due to recent WGDs. How these processes may impact the dynamics gene was studied by comparing patterns structure changes, alternative splicing (AS), codon substitution rates among model grass genomes. In orthologous sets, significantly more acquired lost exonic sequences were detected than...
Summary Recombination affects the fate of alleles in populations by imposing constraints on reshuffling genetic information. Understanding basis these is critical for manipulating recombination process to improve resolution mapping, and reducing negative effects linkage drag deleterious load breeding. Using sequence‐based genotyping a wheat nested association mapping ( NAM ) population 2,100 recombinant inbred lines created crossing 29 diverse lines, we mapped QTL affecting distribution...
Monitoring alien introgressions in crop plants is difficult due to the lack of genetic and molecular mapping information on wild relatives. The tertiary gene pool wheat a very important source variability for improvement against biotic abiotic stresses. By exploring 5Mg short arm (5MgS) Aegilops geniculata, we can apply chromosome genomics discovery SNP markers their use monitoring (Triticum aestivum L). Ae. geniculata Roth (syn. ovata L.; 2n = 4x 28, UgUgMgMg) was flow-sorted from line...
Eight diverse sorghum (Sorghum bicolor L. Moench) accessions were subjected to short-read genome sequencing characterize the distribution of single-nucleotide polymorphisms (SNPs). Two strategies used for DNA library preparation. Missing SNP genotype data imputed by local haplotype comparison. The effect type and genomic diversity on discovery imputation are evaluated. Alignment eight equivalents (6 Gb) public reference revealed 283,000 SNPs at ≥82% confirmation probability. Sequencing from...
Wheat leaf rust, caused by the basidiomycete Puccinia triticina, can cause yield losses of up to 20% in wheat producing regions. During infection, fungus forms haustoria that secrete proteins into plant cell and effect changes transcription, metabolism defense. It is hypothesized new races emerge as a result overcoming resistance via secreted effector proteins. To understand gene expression during infection find genetic differences associated with races, RNA from leaves infected six...
ABSTRACT Wheat ( Triticum aestivum L.) is a primary staple cereal and significant source of mineral nutrients in human diets. Therefore, increasing concentration the essential mineral, Zn, decreasing toxic Cd, could significantly improve health. Because plant mechanisms for uptake translocation Cd Zn are related, we assessed both to evaluate their independence hard winter wheat germplasm. Grain concentrations some genotypes grown Nebraska trials were above Codex guidance level (0.2 mg kg –1...
The soybean cyst nematode (SCN), Heterodera glycines, is one of the most devastating diseases limiting production worldwide. It known that small RNAs, including microRNAs (miRNAs) and interfering RNAs (siRNAs), play important roles in regulating plant growth development, defense against pathogens, responses to environmental changes.In order understand role miRNAs during SCN infection, we analyzed 24 RNA libraries three biological replicates from two cultivars (SCN susceptible KS4607, HG Type...
Abstract Wheat cultivars ‘TAM 111’ and 112’ have been dominantly grown in the Southern U.S. Great Plains for many years due to their high yield drought tolerance. To identify molecular basis genetic control of tolerance these two landmark cultivars, RNA-seq analysis was conducted compare gene expression difference flag leaves under fully irrigated (wet) water deficient (dry) conditions. A total 2254 genes showed significantly altered patterns dry wet conditions cultivars. TAM 111 had 593...
Summary Next‐generation sequencing ( NGS ) provides a powerful tool for the discovery of important genes and alleles in crop plants their wild relatives. Despite great advances technologies, whole‐genome shotgun is cost‐prohibitive species with complex genomes. An attractive option to reduce genome complexity single chromosome prior sequencing. This work describes strategy studying genomes distant relatives wheat by isolating chromosomes from addition or substitution lines, followed sorting...
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Abstract Knowledge of host associations blood-feeding vectors may afford insights into managing disease systems and protecting public health. However, the ability methods to distinguish bloodmeal sources varies widely. We used two methods—Sanger sequencing amplicon deep sequencing—to target a 228 bp region vertebrate Cytochrome b gene determine hosts fed upon by triatomines (n = 115) collected primarily in Texas, USA. Direct Sanger PCR amplicons was successful for 36 samples (31%). revealed...
Generating a contiguous, ordered reference sequence of complex genome such as hexaploid wheat (2n = 6x 42; approximately 17 GB) is challenging task due to its large, highly repetitive, and allopolyploid genome. In wheat, ordering whole-genome or hierarchical shotgun sequencing contigs primarily based on recombination comparative genomics-based approaches. However, genomics approaches are limited syntenic inference suppressed within the pericentromeric regions chromosomes, thus, precise...
Improving the accuracy of PV power prediction is conducive to participation in economic dispatch and market transactions distribution network, as well safe operation grid. Considering that selection highly correlated historical data can improve prediction, this study proposes an integrated method based on a multi-resolution similarity consideration considers both trend detail similarity. Firstly, using irradiance variable, similar-days were selected grey correlation analysis form set similar...
The Arabidopsis ethylene-responsive element-binding factor (AtERF) family of transcription factors has approximately 120 members, all which possess a highly conserved ERF domain. AtERF1, AtERF4, AtEBP and CBF1 are members from different phylogenetic subgroups within the family. Electrophoretic mobility shift assay analyses revealed that domains these four proteins were capable binding specifically to either GCC or dehydration-responsive element (DRE) motifs. In vitro in vivo assays AtERFs...
Two drought-tolerant wheat cultivars, ‘TAM 111’ and 112’, have been widely grown in the Southern Great Plains of U.S. used as parents many breeding programs worldwide. This study aimed to reveal genetic control yield components two cultivars under both dryland irrigated conditions. A mapping population containing 124 F 5:7 recombinant inbred lines (RILs) was developed from cross TAM 112/TAM 111. set 5,948 SNPs 90K iSelect array double digest restriction-site associated DNA sequencing...
Landscape heterogeneity can shape genetic structure and functional connectivity of populations. When this imposes variable costs moving across the landscape, populations be structured according to a pattern "isolation by resistance" (IBR). At same time, divergent local environmental filters limit gene flow, creating an alternative environment" (IBE). Here, we evaluate IBR IBE in insect-pollinated, biennial plant Sabatia angularis (L.) Pursh (Gentianaceae) serpentine grasslands fragmented...