Jitendra P. Khurana
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Constructed Wetlands for Wastewater Treatment
- Genetic Mapping and Diversity in Plants and Animals
- Wheat and Barley Genetics and Pathology
- Plant-Microbe Interactions and Immunity
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Microbial Community Ecology and Physiology
- Coastal wetland ecosystem dynamics
- Peatlands and Wetlands Ecology
- Microbial bioremediation and biosurfactants
- Transgenic Plants and Applications
- Rice Cultivation and Yield Improvement
- Plant Growth Enhancement Techniques
- Mycobacterium research and diagnosis
- Marine and coastal plant biology
University of Delhi
2014-2024
Genomics (United Kingdom)
2021
National Institute of Plant Genome Research
2012
Department of Biotechnology
2012
University of Hyderabad
2012
Indian Institute of Technology Hyderabad
2012
International Centre for Genetic Engineering and Biotechnology
1999
Michigan State University
1984-1992
Smithsonian Environmental Research Center
1992
An ordered draft sequence of the 17-gigabase hexaploid bread wheat (Triticum aestivum) genome has been produced by sequencing isolated chromosome arms. We have annotated 124,201 gene loci distributed nearly evenly across homeologous chromosomes and subgenomes. Comparative analysis subgenomes extant diploid tetraploid relatives showed that high similarity structural conservation are retained, with limited loss, after polyploidization. However, genomes there was evidence dynamic gain,...
The allohexaploid bread wheat genome consists of three closely related subgenomes (A, B, and D), but a clear understanding their phylogenetic history has been lacking. We used assemblies five diploid relatives to analyze genome-wide samples gene trees, as well estimate evolutionary relatedness divergence times. show that the A B genomes diverged from common ancestor ~7 million years ago these gave rise D through homoploid hybrid speciation 1 2 later. Our findings imply present-day is product...
F-box proteins constitute a large family in eukaryotes and are characterized by conserved motif (approximately 40 amino acids). As components of the Skp1p-cullin-F-box complex, critical for controlled degradation cellular proteins. We have identified 687 potential rice (Oryza sativa), model monocotyledonous plant, reiterative database search. Computational analysis revealed presence several other functional domains, including leucine-rich repeats, kelch associated domain, domain unknown...
The basic leucine (Leu) zipper (bZIP) proteins compose a family of transcriptional regulators present exclusively in eukaryotes. bZIP characteristically harbor domain composed two structural features: DNA-binding region and the Leu dimerization region. They have been shown to regulate diverse plant-specific phenomena, including seed maturation germination, floral induction development, photomorphogenesis, are also involved stress hormone signaling. We identified 89 transcription...
Auxin influences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized Arabidopsis detail, but not crop plants. Earlier, we reported the identification characterization of members GH3, Aux/IAA SAUR families rice. In this study, whole genome microarray analysis rice was performed, with aim gaining some insight into mechanism auxin action. A comparison expression profiles untreated auxin-treated seedlings identified 315 probe sets...
The Rice Annotation Project Database (RAP-DB) was created to provide the genome sequence assembly of International Genome Sequencing (IRGSP), manually curated annotation sequence, and other genomics information that could be useful for comprehensive understanding rice biology. Since last publication RAP-DB, IRGSP has been revised reassembled. In addition, a large number rice-expressed tags have released, functional resources produced worldwide. Thus, we thoroughly updated our by manual...
Allohexaploid bread wheat ( Triticum aestivum L.) provides approximately 20% of calories consumed by humans. Lack genome sequence for the three homeologous and highly similar genomes (A, B, D) has impeded expression analysis grain transcriptome. We used previously unknown information to analyze cell type–specific genes in developing identified distinct co-expression clusters reflecting spatiotemporal progression during endosperm development. observed no global but type– stage-dependent...
Important developmental processes in both plants and animals are partly regulated by genes whose expression is modulated at the post-transcriptional level such as RNA interference (RNAi). Dicers, Argonautes RNA-dependent polymerases (RDR) form core components that facilitate gene silencing have been implicated initiation maintenance of trigger molecules, central to process RNAi. Investigations eukaryotes revealed these proteins encoded variable number with showing relatively higher each...
Abstract Background The response regulators represent the elements of bacterial two-component system and have been characterized from dicot plants like Arabidopsis but little information is available on monocots, including cereal crops. aim this study was to characterize type-A regulator genes rice, investigate their expression in various organs as well different hormones, cytokinin, environmental stimuli. Results By analysis whole genome sequence we identified ten encoding based upon high...
We present here the annotation of complete genome rice Oryza sativa L. ssp. japonica cultivar Nipponbare. All functional annotations for proteins and non-protein-coding RNA (npRNA) candidates were manually curated. Functions identified or inferred in 19,969 (70%) proteins, 131 possible npRNAs (including 58 antisense transcripts) found. Almost 5000 annotated protein-coding genes found to be disrupted insertional mutant lines, which will accelerate future experimental validation annotations....
The nuclear-encoded chloroplast small heat shock proteins (sHSPs) are present in all plant species from algae to angiosperms. Expression analysis shows that the wheat chloroplastic sHSP (HSP26) is highly inducible by stress almost vegetative and generative tissues also expressed constitutively certain developmental growth stages. We characterize 26 through transgenic approach using Arabidopsis report cloning of promoter its characterization. Transgenic plants were substantially tolerant...
Rice is an important staple food and, with the smallest cereal genome, serves as a reference species for studies on evolution of cereals and other grasses. Therefore, decoding its entire genome will be prerequisite applied basic research this all cereals.We have determined analyzed complete sequences two chromosomes, 11 12, which total 55.9 Mb (14.3% length), based set overlapping clones. A 5,993 non-transposable element related genes are present these chromosomes. Among them 289 disease...
Tuberculosis (TB) is primarily associated with decline in immune health status. As gut microbiome (GM) implicated the regulation of host immunity and metabolism, here we investigate GM alteration TB patients by 16S rRNA gene whole-genome shotgun sequencing. The study group constituted pulmonary their healthy household contacts as controls (HCs). Significant microbial taxonomic functional capacity was observed active compared to HCs. We that Prevotella Bifidobacterium abundance were HCs,...
Rice is one of the main pillars food security in India. Its improvement for higher yield sustainable agriculture system also vital to provide energy and nutritional needs growing world population, expected reach more than 9 billion by 2050. The high quality genome sequence rice has provided a rich resource mine information about diversity genes alleles which can contribute useful agronomic traits. Defining function each gene regulatory element remains challenge community coming years....
This paper presents the characterization of microbial community responsible for in-situ bioremediation hexachlorocyclohexane (HCH). Microbial structure and function was analyzed using 16S rRNA amplicon shotgun metagenomic sequencing methods three sets soil samples. The samples were collected from a HCH-dumpsite (450 mg HCH/g soil) comprised HCH/soil ratio 0.45, 0.0007, 0.00003, respectively. Certain bacterial; (Chromohalobacter, Marinimicrobium, Idiomarina, Salinosphaera, Halomonas,...
Homeobox genes play a critical role in regulating various aspects of plant growth and development. In the present study, we identified total 107 homeobox rice genome grouped them into ten distinct subfamilies based upon their domain composition phylogenetic analysis. A significantly large number are located duplicated segments genome, which suggests that expansion gene family, part, might have occurred due to segmental duplications rice. Furthermore, microarray analysis was performed...
Reduction in crop yield and quality due to various abiotic stresses is a worldwide phenomenon. In the present investigation, heat shock factor (HSF) gene expressing preferentially developing seed tissues of wheat grown under high temperatures was cloned. This newly identified possesses characteristic domains class A type plant HSFs shows similarity rice OsHsfA2d, hence named as TaHsfA2d. The transcription activity TaHsfA2d confirmed through transactivation assay yeast. Transgenic Arabidopsis...