A5024900384- Light effects on plants
- Wheat and Barley Genetics and Pathology
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Genetics and Plant Breeding
- Circadian rhythm and melatonin
- Plant responses to elevated CO2
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Security and Verification in Computing
- Software Reliability and Analysis Research
- GABA and Rice Research
- Model-Driven Software Engineering Techniques
- Software Engineering Techniques and Practices
- Rice Cultivation and Yield Improvement
- RNA modifications and cancer
- Greenhouse Technology and Climate Control
- Advanced Software Engineering Methodologies
- Advanced Malware Detection Techniques
- Canadian Identity and History
- Soybean genetics and cultivation
- Plant Reproductive Biology
Earlham Institute
2017-2025
Norwich Research Park
2017-2025
University of East Anglia
2017-2024
The Alfred Hospital
2024
Royal Devon & Exeter NHS Foundation Trust
2022
Christ University
2019
University of Liverpool
2008-2018
University of Lethbridge
2000-2018
Deakin University
2018
Skin and Cancer Foundation
2018
Circadian clocks are believed to confer an advantage plants, but the nature of that has been unknown. We show a substantial photosynthetic is conferred by correct matching circadian clock period with external light-dark cycle. In wild type and in long– short–circadian mutants Arabidopsis thaliana , plants matched environment contain more chlorophyll, fix carbon, grow faster, survive better than periods differing from their environment. This explains why gain control.
Bread wheat (Triticum aestivum) is a globally important crop, accounting for 20 per cent of the calories consumed by humans. Major efforts are underway worldwide to increase production extending genetic diversity and analysing key traits, genomic resources can accelerate progress. But so far very large size polyploid complexity bread genome have been substantial barriers analysis. Here we report sequencing its large, 17-gigabase-pair, hexaploid using 454 pyrosequencing, comparison this with...
Abstract Advances in genomics have expedited the improvement of several agriculturally important crops but similar efforts wheat ( Triticum spp.) been more challenging. This is largely owing to size and complexity genome 1 , lack genome-assembly data for multiple lines 2,3 . Here we generated ten chromosome pseudomolecule five scaffold assemblies hexaploid explore genomic diversity among from global breeding programs. Comparative analysis revealed extensive structural rearrangements,...
Seven widely held conceptions about formal methods are challenged. These beliefs variants of the following: can guarantee that software is perfect; they work by proving programs correct; only highly critical systems benefit from their use; involve complex mathematics; increase cost development; incomprehensible to clients; and nobody uses them for real projects. The arguments based on author's experiences. They address bounds methods, identify central role specifications in development...
Understanding genome organization and gene regulation requires insight into RNA transcription, processing modification. We adapted nanopore direct sequencing to examine from a wild-type accession of the model plant Arabidopsis thaliana mutant defective in mRNA methylation (m6A). Here we show that m6A can be mapped full-length mRNAs transcriptome-wide reveal combinatorial diversity cap-associated transcription start sites, splicing events, poly(A) site choice tail length. Loss 3’ untranslated...
Abstract Circadian clocks maintain robust and accurate timing over a broad range of physiological temperatures, characteristic termed temperature compensation. In Arabidopsis thaliana, ambient affects the rhythmic accumulation transcripts encoding clock components TIMING OF CAB EXPRESSION1 (TOC1), GIGANTEA (GI), partially redundant genes CIRCADIAN CLOCK ASSOCIATED1 (CCA1) LATE ELONGATED HYPOCOTYL (LHY). The amplitude peak levels increase for TOC1 GI RNA rhythms as increases (from 17 to...
Summary Food security is a global concern and substantial yield increases in cereal crops are required to feed the growing world population. Wheat one of three most important for human livestock feed. However, complexity genome coupled with decline genetic diversity within modern elite cultivars has hindered application marker‐assisted selection (MAS) breeding programmes. A crucial step successful MAS programmes development cheap easy use molecular markers, such as single‐nucleotide...
Bread wheat is an allopolyploid species with a large, highly repetitive genome. To investigate the impact of selection on variants distributed among homoeologous genomes and to build foundation for understanding genotype-phenotype relationships, we performed population-scale re-sequencing diverse panel lines.A sample 62 lines was re-sequenced using whole exome capture genotyping-by-sequencing approaches. We describe allele frequency, functional significance, chromosomal distribution 1.57...
Abstract Rye ( Secale cereale L.) is an exceptionally climate-resilient cereal crop, used extensively to produce improved wheat varieties via introgressive hybridization and possessing the entire repertoire of genes necessary enable hybrid breeding. allogamous only recently domesticated, thus giving cultivated ryes access a diverse exploitable wild gene pool. To further enhance agronomic potential rye, we produced chromosome-scale annotated assembly 7.9-gigabase rye genome validated its...
Abstract Temperature compensation contributes to the accuracy of biological timing by preventing circadian rhythms from running more quickly at high than low temperatures. We previously identified quantitative trait loci (QTL) with temperature-specific effects on rhythm leaf movement, including a QTL linked transcription factor FLOWERING LOCUS C (FLC). have now analyzed FLC alleles in near-isogenic lines and induced mutants eliminate other candidate genes. showed that lengthened period...
Abstract Many physiological and biochemical processes in plants exhibit endogenous rhythms with a period of about 24 h. Endogenous oscillators called circadian clocks regulate these rhythms. The are synchronized to the periodic environmental changes (e.g. day/night cycles) by specific stimuli; among these, most important is light. Photoreceptors, phytochromes, cryptochromes involved setting clock transducing light signal central oscillator. In this work, we analyzed spatial, temporal,...
Praxis Critical Systems recently developed a secure certification authority for smart cards that had to satisfy performance and usability requirements while meeting stringent security constraints. The authors used systematic process from elicitation through formal specification, user interface prototyping, rigorous design, coding ensure these objectives' achievement. They show how achieves normal commercial productivity can deliver highly reliable system meets all its throughput goals.
Abstract Plants synchronize developmental and metabolic processes with the earth's 24-h rotation through integration of circadian rhythms responses to light. We characterize time for coffee (tic) mutant that disrupts gating, photoperiodism, multiple rhythms, differential effects among rhythms. TIC is distinct in physiological functions genetic map position from other rhythm mutants their homologous loci. Detailed analysis shows chlorophyll a/b-binding protein gene expression requires...
The phytochrome family of photoreceptors are potent regulators plant development, affecting a broad range responses throughout the life cycle, including hypocotyl elongation, leaf expansion and apical dominance. hormone auxin has previously been linked to these phytochrome-mediated responses; however, studies have not identified molecular mechanisms that underpin such extensive cross-talk. In this paper, we show regulates emergence lateral roots, at least partly by manipulating distribution...
Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip now the most widely used transformation method. Although robust, it involves number relatively time-consuming laborious steps, including manipulating an Agrobacterium tumefaciens culture aseptic procedures selection plant lines harboring antibiotic-selection markers. Furthermore, where multiple transgenes are to be introduced, achieving...
Abstract Circadian clocks are required to coordinate metabolism and physiology with daily changes in the environment. Such have several distinctive features, including a free-running rhythm of approximately 24 h ability entrain both light or temperature cycles (zeitgebers). We previously characterized EARLY FLOWERING4 (ELF4) locus Arabidopsis (Arabidopsis thaliana) as being important for robust rhythms. Here, it is shown that ELF4 necessary at least two core clock functions: entrainment an...
Summary Bread wheat, Triticum aestivum , is an allohexaploid composed of the three distinct ancestral genomes, A, B and D. The polyploid nature wheat genome together with its large size has limited our ability to generate significant amount sequence data required for whole studies. Even advent next‐generation sequencing technology, it still relatively expensive sequences more than a few genomes at any one time. To overcome this problem, we have developed targeted‐capture re‐sequencing...
Abstract Plant seeds can sense diverse environmental signals and integrate the information to regulate developmental responses, such as dormancy germination. The circadian clock confers a growth advantage on plants uses for entrainment. Here, we show that normal gene function is essential response dormancy-breaking in seeds. We mutations genes LATE ELONGATED HYPOCOTYL, CIRCADIAN CLOCK ASSOCIATED1 (CCA1), GIGANTEA (GI) cause germination defects low temperature, alternating temperatures, dry...
Abstract The endogenous circadian clock enables organisms to adapt their growth and development environmental changes. Here we describe how the is employed coordinate responses key signal auxin during lateral root (LR) emergence. In model plant, Arabidopsis thaliana , LRs originate from a group of stem cells deep within root, necessitating that new organs emerge through overlying tissues. We report rephased LR development. Metabolite transcript profiling revealed controls levels...
Polyploidy is ubiquitous in eukaryotic plant and fungal lineages, it leads to the co-existence of several copies similar or related genomes one nucleus. In plants, polyploidy considered a major factor successful domestication. However, challenges chromosome folding architecture nucleus establish functional structures.
The Arabidopsis circadian clock orchestrates gene regulation across the day/night cycle. Although a multiple feedback loop circuit has been shown to generate 24-hr rhythm, it remains unclear how robust is in individual cells, or timing coordinated plant. Here we examine activity at single cell level seedlings over several days under constant environmental conditions. Our data reveal oscillations, albeit desynchronised. In particular, observe two waves of activity; one going down, and up...
One of the major challenges for plant scientists is increasing wheat (Triticum aestivum) yield potential (YP). A significant bottleneck YP achieving increased biomass through optimization radiation use efficiency (RUE) along crop cycle. Exotic material such as landraces and synthetic has been incorporated into breeding programmes in an attempt to alleviate this; however, their contribution still unclear. To understand genetic basis accumulation RUE, we applied genome-wide association study...