A5020223478- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Plant Genetic and Mutation Studies
- Radioactive contamination and transfer
- Plant responses to water stress
- Legume Nitrogen Fixing Symbiosis
- Radioactivity and Radon Measurements
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Radiation Effects and Dosimetry
- Genetic and Environmental Crop Studies
- Plant Physiology and Cultivation Studies
- Plant pathogens and resistance mechanisms
- Microtubule and mitosis dynamics
- Plant tissue culture and regeneration
- Soil Carbon and Nitrogen Dynamics
- Aluminum toxicity and tolerance in plants and animals
- Plant Growth Enhancement Techniques
- Chemical Synthesis and Characterization
- Lipid metabolism and biosynthesis
- Plant Disease Resistance and Genetics
- Ion Transport and Channel Regulation
University of Nottingham
2012-2023
Vlaams Instituut voor Biotechnologie
2010
Ghent University
2010
University of Antwerp
2010
Centrum Wiskunde & Informatica
2010
University of Freiburg
2010
University of Warwick
1999
Indian Agricultural Research Institute
1997
Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth development. AUXIN1 (AUX1) has been demonstrated to encode high-affinity auxin carrier. In Arabidopsis thaliana, AUX1 belongs small multigene family comprising four highly conserved genes (i.e., LIKE [LAX] LAX1, LAX2, LAX3). We report that all members this AUX/LAX display uptake functions. Despite the conservation their biochemical function, AUX1, LAX3 have...
In Arabidopsis , lateral root primordia (LRPs) originate from pericycle cells located deep within the parental and have to emerge through endodermal, cortical, epidermal tissues. These overlaying tissues place biomechanical constraints on LRPs that are likely impact their morphogenesis. This study probes interplay between patterns of cell division, organ shape, LRP morphogenesis by exploiting recent advances in live plant imaging image analysis. Our 3D/4D analysis revealed early stage...
Abstract Phosphate (P) is an essential macronutrient for plant growth. Roots employ adaptive mechanisms to forage P in soil. Root hair elongation particularly important since immobile. Here we report that auxin plays a critical role promoting root growth Arabidopsis response low external P. Mutants disrupting synthesis ( taa1 ) and transport aux1 attenuate the response. Conversely, targeting AUX1 expression lateral cap epidermal cells rescues this . Hence from apex differentiation zone...
Auxin represents a key signal in plants, regulating almost every aspect of their growth and development. Major breakthroughs have been made dissecting the molecular basis auxin transport, perception, response. In contrast, how plants control metabolism homeostasis major form indole-3-acetic acid (IAA), remains unclear. this paper, we initially describe function Arabidopsis thaliana gene DIOXYGENASE FOR AUXIN OXIDATION 1 (AtDAO1). Transcriptional translational reporter lines revealed that...
Abstract SHORT-ROOT (SHR) is a well-characterized regulator of radial patterning and indeterminacy the Arabidopsis (Arabidopsis thaliana) primary root. However, its role during elaboration root system architecture remains unclear. We report that indeterminate wild-type was transformed into determinate in shr mutant when growing soil or agar. The growth behavior results from apical meristem failing to initiate cell division following germination. inability reactivate mitotic activity...
Abstract The endogenous circadian clock enables organisms to adapt their growth and development environmental changes. Here we describe how the is employed coordinate responses key signal auxin during lateral root (LR) emergence. In model plant, Arabidopsis thaliana , LRs originate from a group of stem cells deep within root, necessitating that new organs emerge through overlying tissues. We report rephased LR development. Metabolite transcript profiling revealed controls levels...
Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental tissues. LRP emerge through overlying tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin-inducible auxin influx carrier LAX3 plays a key role concentrating this signal LRP. Delimiting expression to two files new is crucial ensure that auxin-regulated occurs solely along their shared walls. Multiscale modeling has predicted highly focused pattern of...
In plants, postembryonic formation of new organs helps shape the adult organism. This requires tight regulation when and where a organ is formed coordination underlying cell divisions. To build root system, lateral roots are continuously developing, this process asymmetric division in adjacent pericycle cells. We identified EXPANSIN A1 (EXPA1) as wall modifying enzyme controlling divisions marking initiation. Loss EXPA1 leads to defects first radial swelling during auxin-driven formation....
Oxygen is a key signalling component of plant biology, and whilst an oxygen-sensing mechanism was previously described in Arabidopsis thaliana, features the associated PLANT CYSTEINE OXIDASE (PCO) N-degron pathway Group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factor substrates remain untested or unknown. We demonstrate that ERFVIIs show non-autonomous activation root hypoxia tolerance are essential for development survival under oxygen limiting conditions soil. determine combined...
Summary We have analysed the circadian rhythm of Arabidopsis thaliana leaf movements in accession Cvi from Cape Verde Islands, and commonly used laboratory strains Columbia (Col) Landsberg ( erecta ) (L er ), which originated Northern Europe. The parental lines similar rhythmic periods, but progeny crosses among them reveal extensive variation for this trait. An analysis 48 L /Cvi recombinant inbred (RILs) a further 30 /Col RILs allowed us to locate four putative quantitative trait loci...
The root meristem of the Arabidopsis thaliana mature embryo is a highly organized structure in which individual cell shape and size must be regulated co-ordination with surrounding cells. objective this study was to determine role AUX1 LAX family auxin import carriers during establishment embryonic pattern.The radicle apex single multiple aux1 lax mutant embryos used evaluate effect gene upon organization cap size, number size.It demonstrated here that mutations within are associated changes...
SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR SCR genes results disorganization quiescent center loss activity, resulting cessation This paper reports on role development leaves, which, contrast to root, have a determinate growth pattern lack persistent niche. Our demonstrate that inhibition leaf shr scr mutants is not secondary effect compromised but...
Summary Plant root system plasticity is critical for survival in changing environmental conditions. One important aspect of architecture lateral development, a complex process regulated by hormone, and protein signalling pathways. Here we show, using molecular genetic approaches, that the MYB transcription factor At 93 novel negative regulator development Arabidopsis. We identify as an interaction partner lateral‐root‐promoting ARABIDILLO proteins. Atmyb93 mutants have faster developmental...
Lateral root (LR) formation is an important determinant of system architecture. In Arabidopsis, LRs originate from pericycle cells, which undergo a programme morphogenesis to generate new LR meristem. Despite its importance for meristem organisation, the onset organizing center (termed quiescent center; QC) during remains unclear. Here, we used live 3D confocal imaging monitor cell organization and identity acquisition development. Our dynamic observations revealed early phase late as...
Aquaporins (AQPs) are water channels allowing fast and passive diffusion of across cell membranes. It was hypothesized that AQPs contribute to elongation processes by influx the plasma membrane tonoplast maintain adequate turgor pressure. Here, we report that, in Arabidopsis (Arabidopsis thaliana), highly abundant AQP isoforms AtTIP1;1, AtTIP1;2, AtTIP2;1 facilitate emergence new lateral root primordia (LRPs). The number roots strongly reduced triple tip mutant, whereas single, double,...
Stomata are formed by a pair of guard cells which have thickened, elastic cell walls to withstand the large increases in turgor pressure that be generated open pore they surround. We characterized FOCL1, cell-expressed, secreted protein with homology Hyp-rich wall proteins. FOCL1-GFP localizes outer cuticular ledge and plants lacking FOCL1 produce stomata without ledge. Instead majority stomatal pores entirely covered over continuous fusion cuticle, consequently decreased levels...
The role of root phenes in nitrogen (N) acquisition and biomass production was evaluated 10 contrasting natural accessions Arabidopsis thaliana L. Seedlings were grown on vertical agar plates with two different nitrate supplies. low N treatment increased the to shoot ratio promoted proliferation lateral roots hairs. cost a larger system did not impact biomass. Greater could be achieved through length or specific hair characteristics. A greater number hairs may provide low-resistance pathway...
• Ingestion of caesium (Cs) radioisotopes poses a health risk to humans. Crop varieties that accumulate less Cs in their edible tissues may provide useful countermeasure. This study was performed determine whether quantitative genetics on model plant (Arabidopsis thaliana) might inform such 'safe'-crop strategies. Arabidopsis accessions and recombinant inbred lines (RILs), from Landsberg erecta (Ler) × Cape Verdi Island (Cvi), Ler Columbia (Col), Niederzenz (Nd) Col mapping populations, were...
Phosphite represents a reduced form of phosphate that belongs to class crop growth-promoting chemicals termed biostimulants. Previous research has shown phosphite application can enhance root growth, but its underlying mechanism, especially during environmental stresses, remains elusive. To uncover this, we undertook series morphological and physiological analyses under nutrient, water heat stresses following foliar in wheat. Non-invasive 3D imaging system architecture directly soil using...
Rhizoctonia solani is a necrotrophic, soilborne fungal pathogen associated with significant establishment losses in Brassica napus (oilseed rape; OSR). The anastomosis group (AG) 2-1 of R. the most virulent to OSR, causing damping-off, root and hypocotyl rot, seedling death. Resistance AG2-1 OSR has not been identified, regulation defense its adapted pathogen, AG2-1, investigated. In this work, we used confocal microscopy visualize progress infection by sclerotia on B. varieties contrasting...