A5085922198- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Nuclear physics research studies
- Particle Detector Development and Performance
- Near-Field Optical Microscopy
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Diamond and Carbon-based Materials Research
- Nuclear Physics and Applications
- Microtubule and mitosis dynamics
- High-Energy Particle Collisions Research
- Particle physics theoretical and experimental studies
- Orbital Angular Momentum in Optics
- Radiation Detection and Scintillator Technologies
- Force Microscopy Techniques and Applications
- Advanced Electron Microscopy Techniques and Applications
- Optical Coherence Tomography Applications
- Astronomical and nuclear sciences
- Atomic and Subatomic Physics Research
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Atomic and Molecular Physics
- Nuclear reactor physics and engineering
- Insect Resistance and Genetics
- Microfluidic and Bio-sensing Technologies
Goethe University Frankfurt
2016-2025
Lucerne University of Applied Sciences and Arts
2022
Goethe Institute
2021
RELX Group (United States)
2019
University of Utah
2018
European Molecular Biology Organization
1993-2015
Institut de Biologie Moléculaire et Cellulaire
2014
European Molecular Biology Laboratory
2002-2011
Mitre (United States)
2011
Frankfurt Institute for Advanced Studies
2011
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination microscopy (SPIM) generate multidimensional images samples up a few millimeters in size. The system combines two-dimensional with orthogonal camera-based detection achieve high-resolution, optically sectioned throughout the sample, minimal photodamage at speeds capable capturing transient phenomena. used...
A long-standing goal of biology is to map the behavior all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization movement in entire wild-type mutant zebrafish embryos over first 24 hours development. Multiview vivo imaging at 1.5 billion voxels per minute provides “digital embryos,” that is, comprehensive databases cell positions, divisions, migratory tracks. Our analysis global division patterns reveals...
The design, construction, and commissioning of the ALICE Time-Projection Chamber (TPC) is described. It main device for pattern recognition, tracking, identification charged particles in experiment at CERN LHC. TPC cylindrical shape with a volume close to 90 m^3 operated 0.5 T solenoidal magnetic field parallel its axis. In this paper we describe detail design considerations detector operation extreme multiplicity environment central Pb--Pb collisions LHC energy. implementation resulting...
SUMMARY The effect of refractive‐index mismatch, as encountered in the observation biological specimens, on image acquisition process confocal fluorescence microscopy is investigated theoretically. analysis takes vectorial properties light into account and valid for high numerical apertures. Quantitative predictions decrease resolution, intensity drop shift focus are given practical situations. When observing with a aperture 1·3 (oil immersion) an excitation wavelength 514 nm centre shifts...
In a 4Pi confocal fluorescence microscope two opposing objective lenses were used to illuminate fluorescent object from both sides and collect the emissions on sides. Constructive interference of either illumination wave fronts in common focus or detection detector pinhole resulted an axial resolution approximately four times higher than that microscope. A precise uses simultaneous was built. The full width at half-maximum depth discrimination determined experimentally be 110 nm when with...
Studies of the developing trophectoderm in mouse embryo have shown that extensive cellular remodeling occurs during epithelial formation. In this investigation, confocal immunofluorescence microscopy is used to examine three-dimensional changes architecture take place polarization a terminally differentiated cell line. Madin-Darby canine kidney cells were plated at low density on permeable filter supports. Antibodies specifically recognize components tight junction, adherens microtubules,...
To study the intracellular transport of newly synthesized sphingolipids in epithelial cells we have used a fluorescent ceramide analog, N-6[7-nitro-2,1,3-benzoxadiazol-4-yl] aminocaproyl sphingosine (C6-NBD-ceramide; Lipsky, N. G., and R. E. Pagano, 1983, Proc. Natl. Acad. Sci. USA, 80:2608-2612) as probe. This was readily taken up by filter-grown Madin-Darby canine kidney (MDCK) from liposomes at 0 degrees C. After penetration into cell, probe accumulated Golgi area temperatures between 20...
In eukaryotic cells, the onset of mitosis involves cyclin molecules which interact with proteins cdc2 family to produce active kinases. vertebrate A dependent kinases become in S- and pro-phases, whereas a B-dependent kinase is mostly metaphase. It has recently been shown that, when added Xenopus egg extracts, bacterially produced A- B-type cyclins associate predominantly same catalytic subunit, namely p34cdc2, induce its histone H1 activity different kinetics. Here, we show that cell free...
We visualized a fluorescent-protein (FP) fusion to Rab6, Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi cell periphery. TCs specifically accumulated retrograde cargo, wild-type Shiga toxin B-fragment (STB), during STB endoplasmic reticulum (ER). associated intimately ER, and entered ER via specialized peripheral regions that FP-Rab6. Microinjection of antibodies block...
During unequal cell divisions a mitotic spindle is eccentrically positioned before cleavage. To determine the basis of net force imbalance that causes displacement in one-cell Caenorhabditis elegans embryos, we fragmented centrosomes with an ultraviolet laser. Analysis mean and variance fragment speeds suggests due to larger number generators pulling on astral microtubules posterior aster relative anterior aster. Moreover, activation heterotrimeric guanine nucleotide- binding protein...
Lateral root formation in plants can be studied as the process of interaction between chemical signals and physical forces during development. primordia grow through overlying cell layers that must accommodate this incursion. Here, we analyze responses endodermis, immediate neighbor to an initiating lateral root. Endodermal cells lose volume, change shape, relinquish their tight junction-like diffusion barrier make way for emerging primordium. feedback is absolutely required initiation...
The mechanics of the actin cytoskeleton have a central role in regulation cells and tissues, but details how molecular sensors recognize deformations forces are elusive. By performing laser nanosurgery cultured epithelial fibroblasts, we show that retraction stress fibers (SFs) is restricted to proximity cut new adhesions form at retracting end. This suggests SFs attached substrate. A computational model for confirms this hypothesis predicts distribution propagation contractile along SF. We...
In Arabidopsis , lateral root primordia (LRPs) originate from pericycle cells located deep within the parental and have to emerge through endodermal, cortical, epidermal tissues. These overlaying tissues place biomechanical constraints on LRPs that are likely impact their morphogenesis. This study probes interplay between patterns of cell division, organ shape, LRP morphogenesis by exploiting recent advances in live plant imaging image analysis. Our 3D/4D analysis revealed early stage...
Cancer cells in poorly vascularized tumor regions need to adapt an unfavorable metabolic microenvironment. As distance from supplying blood vessels increases, oxygen and nutrient concentrations decrease cancer react by stopping cell cycle progression becoming dormant. cytostatic drugs mainly target proliferating cells, dormancy is considered as a major resistance mechanism this class of anti-cancer drugs. Therefore, substances that have the potential enhance cytostatic-based chemotherapy...