Narinder Singh

ORCID: 0000-0002-4370-8565
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About
Contact & Profiles
Research Areas
  • Wheat and Barley Genetics and Pathology
  • Plant Disease Resistance and Genetics
  • Genetics and Plant Breeding
  • Genetic Mapping and Diversity in Plants and Animals
  • Animal Nutrition and Physiology
  • Protein Structure and Dynamics
  • Plant Virus Research Studies
  • Plant Pathogenic Bacteria Studies
  • Plant Pathogens and Fungal Diseases
  • Enzyme Structure and Function
  • Plant Pathogens and Resistance
  • Chromosomal and Genetic Variations
  • Ruminant Nutrition and Digestive Physiology
  • Environmental Toxicology and Ecotoxicology
  • Aquaculture Nutrition and Growth
  • Genetic and Environmental Crop Studies
  • Agricultural Science and Fertilization
  • Amino Acid Enzymes and Metabolism
  • Plant Disease Management Techniques
  • DNA and Nucleic Acid Chemistry
  • Scientific Computing and Data Management
  • Livestock Management and Performance Improvement
  • Bone Metabolism and Diseases
  • Phytochemical compounds biological activities
  • Agricultural Economics and Practices

Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu
2023

Kansas State University
2015-2022

Bayer (United States)
2019-2022

National Institute of Ayurveda
2021

Sharda University
2020

New Mexico State University
2014-2019

ICAR Research Complex for NEH Region
2019

Planta
2014

Washington State University
2014

University of Kansas
1988-2000

Abstract Aegilops tauschii , the diploid wild progenitor of D subgenome bread wheat, is a reservoir genetic diversity for improving wheat performance and environmental resilience. Here we sequenced 242 Ae. accessions compared them to characterize genomic diversity. We found that rare lineage geographically restricted present-day Georgia contributed in independent hybridizations gave rise modern wheat. Through k -mer-based association mapping, identified discrete regions with candidate genes...

10.1038/s41587-021-01058-4 article EN cc-by Nature Biotechnology 2021-11-01

The ultrafiltration function of the glomerular basement membrane (GBM) kidney is impaired in genetic and acquired diseases that affect type IV collagen. GBM composed five (α1 to α5) six chains collagen, organized into an α1·α2(IV) α3·α4·α5(IV) network. In Alport syndrome, mutations any genes encoding α3(IV), α4(IV), α5(IV) cause absence α3·α4·α5 network, which leads progressive renal failure. present study, molecular mechanism underlying network defect was explored by further...

10.1074/jbc.m004569200 article EN cc-by Journal of Biological Chemistry 2000-09-01

Genebanks are valuable resources for crop improvement through the acquisition, ex-situ conservation and sharing of unique germplasm among plant breeders geneticists. With over seven million existing accessions increasing storage demands costs, genebanks need efficient characterization curation to make them more accessible usable reduce operating so that community can most effectively leverage this vast resource untapped novel genetic diversity. However, inconsistent documentation often...

10.1038/s41598-018-37269-0 article EN cc-by Scientific Reports 2019-01-24

Abstract The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. confers at both seedling and adult stages, it is broadly effective against all races tested date. has been extensively in CIMMYT international breeding program with resulting cultivars deployed several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for . Overexpression of nucleotide-binding...

10.1038/s41467-022-30784-9 article EN cc-by Nature Communications 2022-06-01

Aegilops tauschii, the D-genome progenitor of Triticum aestivum, encompasses huge diversity for various traits potential economic importance such as yield, biotic and abiotic stress tolerance, quality nutrition. In present study, variation grain size in Ae. tauschii germplasm was studied its genetic basis dissected using genome wide association study (GWAS). Grain length, width weight evaluated 177 accessions over three years showed near normal distribution with 1.74, 1.75 2.82-fold...

10.3389/fpls.2017.00886 article EN cc-by Frontiers in Plant Science 2017-05-31

Aegilops tauschii, the D-genome donor of bread wheat, Triticum aestivum, is a storehouse genetic diversity, and an important resource for future wheat improvement. Genomic population analysis 549 Ae. tauschii 103 accessions was performed by using 13,135 high quality SNPs. Population structure, principal component, cluster confirmed differentiation into two lineages; lineage 1 (L1) 2 (L2), latter being donor. Lineage L1 contributes only 2.7% total introgression from set U.S. winter lines,...

10.3389/fpls.2019.00009 article EN cc-by Frontiers in Plant Science 2019-01-25

10.1016/0076-6879(95)59045-5 article EN Methods in enzymology on CD-ROM/Methods in enzymology 1995-01-01

Summary Next‐generation sequencing ( NGS ) provides a powerful tool for the discovery of important genes and alleles in crop plants their wild relatives. Despite great advances technologies, whole‐genome shotgun is cost‐prohibitive species with complex genomes. An attractive option to reduce genome complexity single chromosome prior sequencing. This work describes strategy studying genomes distant relatives wheat by isolating chromosomes from addition or substitution lines, followed sorting...

10.1111/tpj.13036 article EN The Plant Journal 2015-09-26

A-genome diploid wheats represent the earliest domesticated and cultivated wheat species in Fertile Crescent include donor of A sub-genome. The encompass einkorn (Triticum monococcum L. subsp. monococcum), wild (T. aegilopoides (Link) Thell.), Triticum urartu. We evaluated collection 930 accessions Wheat Genetics Resource Center (WGRC) using genotyping by sequencing identified 13,860 curated single-nucleotide polymorphisms. Genomic analysis detected misclassified genetically identical (>99%)...

10.1093/plphys/kiac006 article EN cc-by PLANT PHYSIOLOGY 2022-01-28

The domestication bottleneck has reduced genetic diversity inwheat, necessitating the use of wild relatives in breeding programs. Wild tetraploid wheat are widely used programs but with morphological characters, it is difficult to distinguish these, resulting misclassification/mislabeling or duplication accessions Gene bank.The study aims exploreGenotyping by sequencing (GBS) characterize and domesticated generate a core set be program.TASSEL-GBS pipeline was for SNP discovery, fastStructure...

10.1016/j.jare.2022.08.020 article EN cc-by-nc-nd Journal of Advanced Research 2022-09-07

Iris yellow spot is an economically important disease of onion that reduces bulb size and yield difficult to control. The spread by Thrips tabaci (onion thrips) symptoms are exacerbated hot, dry climatic conditions also favor rapid thrips multiplication make control programs less effective. Currently, no cultivar resistant the and/or thrips. Certain foliar characteristics have shown nonpreferential feeding activity may be first step in developing (IYS)-tolerant cultivars. Seventy-five PI...

10.21273/hortsci.49.10.1253 article EN HortScience 2014-10-01

Abstract Aegilops tauschii, the diploid wild progenitor of D-subgenome bread wheat, constitutes a reservoir genetic diversity for improving wheat performance and environmental resilience. To better define understand this diversity, we sequenced 242 Ae. tauschii accessions compared them to D-subgenome. We characterized rare, geographically-restricted lineage discovered that it contributed D-subgenome, thereby elucidating origin from at least two independent hybridizations. then used k...

10.1101/2021.01.31.428788 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2021-02-01

Iris yellow spot (IYS) disease, caused by virus (IYSV), results in irregular and diamond-shaped, chlorotic, necrotic lesions on the leaves seedstalks of onions ( Allium cepa L.). These reduce leaf photosynthetic area ultimately onion bulb size yield from larger classes. IYSV is vectored thrips Thrips tabaci L.) that are difficult to control under certain environmental conditions. Currently, no cultivar resistant disease symptoms, virus, and/or thrips. Twenty-one cultivars 17 germplasm lines...

10.21273/hortsci11770-17 article EN HortScience 2017-04-01

Iris yellow spot (IYS), a disease caused by virus (IYSV) and spread onion thrips, is devastating of bulb seed production. The development germplasm resistant to IYS and/or thrips crucial production, since host plant resistance unknown for both pests. During the summer 2010 2012, plants with fewer symptoms were selected from screening introduction accessions (PIs) first-generation selections, respectively. resulting progeny these evaluated during summers 2013 2014 numbers symptom expression,...

10.3390/horticulturae5010018 article EN cc-by Horticulturae 2019-02-16

ABSTRACT Genebanks are valuable resources for crop improvement through the acquisition, ex-situ conservation and sharing of unique germplasm among plant breeders geneticists. With over seven million existing accessions increasing storage demands costs, genebanks need efficient characterization curation to make them more accessible usable reduce operating so that community can most effectively leverage this vast resource untapped novel genetic diversity. However, inconsistent documentation...

10.1101/410779 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2018-09-06

The genetic variability of 'Candidatus Liberibacter asiaticus' (CLas) population associated with huanglongbing (HLB) disease citrus in North Eastern (NE) region India, a geographically locked region, and home for the diversity many species was analyzed on basis tandem repeat numbers (TRN) variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves NE Hill (NEH) India were single amplicon group, but there remarkable TRNs. TRN HLB-associated varied 0-21 two...

10.5423/ppj.oa.03.2019.0061 article EN cc-by-nc The Plant Pathology Journal 2019-12-01

In previous transient state kinetic work from this laboratory, we proposed a new mechanism for the glutamate dehydrogenase-catalyzed oxidative deamination reaction involving an initial replacement of proton lysine 126 by single bound water molecule, followed closure active site cleft and expulsion bulk water, providing hydrophobic environment ensuing hydride transfer step. Here, report results further fluorescence, absorbance, isotope effect studies, which demonstrate occurrence unusual...

10.1016/s0021-9258(18)43921-x article EN cc-by Journal of Biological Chemistry 1994-11-01

We have previously characterized the thermodynamic relationships which govern dissociation of NADPH from bovine liver glutamate dehydrogenase and allosteric control that mechanically physiologically important process by a variety effectors. found cooperative occupancy specific anion binding, while second acetate binding site disrupts opposes those effects (Singh & Fisher, 1994). report here results transient-state studies on kinetics various processes involved in this complex equilibrium....

10.1021/bi00200a019 article EN Biochemistry 1994-08-30

Short read sequencing technologies, such as genotyping-by-sequencing (GBS), have been utilized in genetic mapping, marker development, and population genomic studies. High-throughput multiplexing capability coupled with low cost make GBS an appropriate tool for molecular research. Here, we present the application of to characterize wheat aneuploid stocks detect chromosomal aberrations including aneuploidy deletions. These aneuploids are important resource that used genetics genomics studies...

10.1186/s13007-020-00588-3 article EN cc-by Plant Methods 2020-04-06

Two experiments were laid out to study the effect of potting media and hormonal treatment on nursery performance using seed branch cuttings respectively in .In first experiment, four media-pure soil, soil: sand (1:1), :FYM (1:1:1) Murraya koenigii :vermicompost used five replications.In second stem taken from healthy plants treated with thirteen concentrations: IBA (500, 1000, 1500 2000 ppm), NAA ppm) control (no treatment).This experiment had three replications.The seeds sown sand:...

10.55362/ije/2023/3851 article EN Indian Journal of Ecology 2023-02-28

The usefulness of the indicator dye method for detection transient enzyme-product intermediates has been very limited due to near impossibility resolving apparent single exponential time courses resulting from sequences steps linked by rather similar rate constants. We propose here a novel approach, proton-product course method, procedure which can extract great deal mechanistic information remains buried in conventional proton release-time measurements. involves nothing more than measuring...

10.1016/s0021-9258(18)37841-4 article EN cc-by Journal of Biological Chemistry 1988-08-01
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