Ngonidzashe Kangara
A5066766375- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Plant-Microbe Interactions and Immunity
- Genetic and Environmental Crop Studies
- Plant Virus Research Studies
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Yeasts and Rust Fungi Studies
- Horticultural and Viticultural Research
- Genetics and Plant Breeding
Norwich Research Park
2018-2023
John Innes Centre
2018-2023
Abstract Aegilops tauschii , the diploid wild progenitor of D subgenome bread wheat, is a reservoir genetic diversity for improving wheat performance and environmental resilience. Here we sequenced 242 Ae. accessions compared them to characterize genomic diversity. We found that rare lineage geographically restricted present-day Georgia contributed in independent hybridizations gave rise modern wheat. Through k -mer-based association mapping, identified discrete regions with candidate genes...
Abstract The wild relatives and progenitors of wheat have been widely used as sources disease resistance ( R ) genes. Molecular identification characterization these genes facilitates their manipulation tracking in breeding programmes. Here, we develop a reference-quality genome assembly the diploid relative Aegilops sharonensis use positional mapping, mutagenesis, RNA-Seq transgenesis to identify stem rust gene Sr62 , which has also transferred common wheat. This encodes tandem kinase,...
Wheat stem rust, a devastating disease of wheat and barley caused by the fungal pathogen Puccinia graminis f. sp. tritici, was largely eradicated in Western Europe during mid-to-late twentieth century. However, isolated outbreaks have occurred recent years. Here we investigate whether lack resistance modern European varieties, increased presence its alternate host barberry changes climatic conditions could be facilitating resurgence. We report first rust occurrence United Kingdom nearly 60...
Genetic resistance is the most economic and environmentally sustainable approach for crop disease protection. Disease (R) genes from wild relatives are a valuable resource breeding resistant crops. However, introgression of R into crops lengthy process often associated with co-integration deleterious linked 1, 2 pathogens can rapidly evolve to overcome when deployed singly 3 . Introducing multiple cloned as stack would avoid linkage drag delay emergence resistance-breaking pathogen races 4...
Wheat stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), is regaining prominence due to recent emergence of hypervirulent isolates and epidemics in Africa, Europe Central Asia. The development deployment wheat cultivars with multiple resistance (Sr) genes stacked together will provide durable resistance. However, certain disease can suppress each other or fail particular genetic backgrounds. Therefore, function Sr gene must be confirmed after incorporation into an Sr-gene...
Abstract Aegilops tauschii, the diploid wild progenitor of D-subgenome bread wheat, constitutes a reservoir genetic diversity for improving wheat performance and environmental resilience. To better define understand this diversity, we sequenced 242 Ae. tauschii accessions compared them to D-subgenome. We characterized rare, geographically-restricted lineage discovered that it contributed D-subgenome, thereby elucidating origin from at least two independent hybridizations. then used k...
Abstract The wild relatives and progenitors of wheat have been widely used as sources disease resistance ( R ) genes. Molecular identification characterization these genes facilitates their manipulation tracking in breeding programmes. We developed a reference-quality genome assembly the diploid relative Aegilops sharonensis positional mapping, mutagenesis, RNA-Seq transgenesis to identify stem rust gene Sr62 , which was also transferred common wheat. This encodes tandem kinase, homologues...
Abstract To safeguard bread wheat against pests and diseases, breeders have introduced over 200 resistance genes into its genome, thus nearly doubling the number of designated in gene pool. Isolating these facilitates their fast-tracking breeding programs incorporation polygene stacks for more durable resistance. We cloned stem rust Sr43 , which was crossed from wild grass Thinopyrum elongatum . encodes a protein kinase fused to two domains unknown function. The gene, is unique Triticeae,...