A5019853316- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Genetic and phenotypic traits in livestock
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Crop Yield and Soil Fertility
- Plant Pathogens and Fungal Diseases
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Gene expression and cancer classification
- Lipid metabolism and biosynthesis
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Plant Water Relations and Carbon Dynamics
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Mitochondrial Function and Pathology
- Rice Cultivation and Yield Improvement
- Plant tissue culture and regeneration
- Sunflower and Safflower Cultivation
- Plant Stress Responses and Tolerance
KWS Saat (Germany)
2015-2025
John Innes Centre
2018
University of Hohenheim
2011
Significance Northern corn leaf blight (NCLB) is one of the most devastating fungal diseases maize. The Htn1 disease resistance gene confers quantitative field against NCLB isolates. Here we show that encodes a putative wall-associated receptor-like kinase (RLK). RLKs act as important components first tier plant innate immune system by perceiving pathogen- or host-derived elicitors on cell surface. are often associated with to nonadapted pathogens and component nonhost resistance. Our work...
High density genotyping data are indispensable for genomic analyses of complex traits in animal and crop species. Maize is one the most important plants worldwide, however a high SNP array analysis its large highly dynamic genome was not available so far. We developed maize composed 616,201 variants (SNPs small indels). Initially, 57 M were discovered by sequencing 30 representative temperate lines then stringently filtered sequence quality scores predicted conversion performance on...
In sexually reproducing organisms, meiotic crossovers ensure the proper segregation of chromosomes and contribute to genetic diversity by shuffling allelic combinations. Such reassortment is exploited in breeding combine favorable alleles, research identify factors underlying traits interest via linkage or association-based approaches. Crossover numbers distributions along vary between species, but little known about their intraspecies variation. Here, we report on variation recombination...
Abstract The efficiency of marker-assisted prediction phenotypes has been studied intensively for different types plant breeding populations. However, one remaining question is how to incorporate and counterbalance information from biparental multiparental populations into model training genome-wide prediction. To address this question, we evaluated testcross performance 1652 doubled-haploid maize (Zea mays L.) lines that were genotyped with 56,110 single nucleotide polymorphism markers...
Abstract The diversity of maize ( Zea mays ) is the backbone modern heterotic patterns and hybrid breeding. Historically, US farmers exploited this variability to establish today’s highly productive Corn Belt inbred lines from blends dent flint germplasm pools. Here, we report de novo genome sequences four European assembled pseudomolecules with scaffold N50 ranging 6.1 10.4 Mb. Comparative analyses two explains pronounced differences between both germplasms. While overall syntenic order...
An association study conducted on 375 maize inbred lines indicates a strong relationship between Vgt1 polymorphisms and flowering time, extending former quantitative trait loci (QTL) mapping results. Analysis of allele frequencies in landrace collection supports key role altilatitudinal adaptation.
Abstract Association mapping has permitted the discovery of major QTL in many species. It can be applied to existing populations and, as a consequence, it is generally necessary take into account structure and relatedness among individuals statistical model control false positives. We analytically studied power association studies by computing noncentrality parameter tests its relationship with parameters characterizing diversity (genetic differentiation between groups allele frequencies)...
Abstract Multiparental designs combined with dense genotyping of parents have been proposed as a way to increase the diversity and resolution quantitative trait loci (QTL) mapping studies, using methods combining linkage disequilibrium information analysis (LDLA). Two new nested association adapted European conditions were derived from complementary dent flint heterotic groups maize (Zea mays L.). Ten biparental families (N = 841) 11 811) genotyped 56,110 single nucleotide polymorphism...
Breeding for cold tolerance in maize promises to allow increasing growth area and production temperate zones. The objective of this research was conduct genome-wide association analyses (GWAS) inbred lines find strategies pyramiding genes tolerance. Two panels 306 dent 292 European flint were evaluated per se testcrosses under control conditions a chamber. We recorded indirect measures as the traits number days from sowing emergence, relative leaf chlorophyll content or quantum efficiency...
Summary Wall‐associated kinases ( WAK s) have recently been identified as major components of fungal and bacterial disease resistance in several cereal crop species. However, the molecular mechanisms ‐mediated remain largely unknown. Here, we investigated function maize gene Zm ‐ RLK 1 Htn1 ) that confers quantitative to northern corn leaf blight NCLB caused by hemibiotrophic pathogen Exserohilum turcicum . was found localize plasma membrane its presence resulted a modification infection...
Abstract Genetic variation is of crucial importance for crop improvement. Landraces are valuable sources diversity, but quantitative traits efficient strategies their targeted utilization lacking. Here, we map haplotype-trait associations at high resolution in ~1000 doubled-haploid lines derived from three maize landraces to make native diversity early development accessible elite germplasm A comparative genomic analysis the discovered haplotypes landrace-derived and a panel 65 breeding...
Key message Doubled-haploid libraries from landraces capture native genetic diversity for a multitude of quantitative traits and make it accessible breeding genome-based studies. Abstract Maize comprise large allelic diversity. We created doubled-haploid (DH) three European flint maize characterized them with respect to their molecular diversity, population structure, trait means, variances, correlations. In total, 899 DH lines were evaluated using high-quality genotypic multi-environment...
Northern corn leaf blight, caused by the fungal pathogen Setosphaeria turcica (anamorph Exserohilum turcicum), is one of most devastating foliar diseases maize (Zea mays). Four genes Ht1, Ht2, Ht3 and Htn1 represent major sources genetic resistance against hemibiotrophic fungus S. turcica. Differential lines containing these also form basis to classify races. Here, we show that Ht2 are identical allelic previously cloned gene. Using a map-based cloning approach Targeting Induced Local...
Abstract Background Cell-wall digestibility is the major target for improving feeding value of forage maize. An understanding molecular basis cell-wall crucial towards breeding highly digestible Results 865 candidate ESTs were selected according to analysis expression profiles in 1) three sets brown-midrib isogenic lines genetic background inbreds 1332 (1332 and bm3 ), 5361 (5361 F2 (F2, bm1 , bm2 2) contrasting extreme FD (Flint × Dent, AS08 AS 06), DD1 (Dent AS11 AS09), DD2 AS29 AS30)...
In genomic prediction, an important measure of accuracy is the correlation between predicted and true breeding values. Direct computation this quantity for real datasets not possible, because value unknown. Instead, values observed phenotypic values, called predictive ability, often computed. order to indirectly estimate accuracy, latter usually divided by square root heritability. study we use simulation evaluate estimates seven methods, four (1 4) which heritability divide ability computed...
Gibberella ear rot (GER) of maize ( Zea mays L.) caused by Fusarium graminearum Schwabe is a major disease in Europe that reduces grain yield and leads to contamination with deoxynivalenol (DON) zearalenone (ZEA), two important mycotoxins. The objectives our study were (i) estimate quantitative‐genetic parameters for GER severity DON ZEA contaminations, (ii) map quantitative trait loci (QTL) resistance low (iii) examine the prospects marker‐assisted selection (MAS) these traits. materials...
The genetic dissection of root architecture and functions allows for a more effective informed design novel ideotypes paves the way to evaluate their effects on crop resilience number abiotic stresses. In maize, limited attention has been devoted analysis diversity at early stage. difference in embryonic (including seminal primary) between maize reference line B73 (which mostly develops three roots) landrace Gaspé Flint (with virtually no was genetically dissected using collection...
Abstract Background Forage quality of maize is influenced by both the content and structure lignins in cell wall. Biosynthesis monolignols, constituting complex lignins, catalyzed enzymes phenylpropanoid pathway. Results In present study we have amplified partial genomic fragments six putative pathway genes a panel elite European inbred lines ( Zea mays L.) contrasting forage traits. Six loci, encoding C4H, 4CL1, 4CL2, C3H, F5H, CAD, displayed different levels nucleotide diversity linkage...