A5007623662- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
- Agronomic Practices and Intercropping Systems
- Mycorrhizal Fungi and Plant Interactions
- Mycotoxins in Agriculture and Food
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Plant pathogens and resistance mechanisms
- Rice Cultivation and Yield Improvement
- Plant Micronutrient Interactions and Effects
- Plant Molecular Biology Research
- Telomeres, Telomerase, and Senescence
- Genetic Mapping and Diversity in Plants and Animals
- Transgenic Plants and Applications
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Gene expression and cancer classification
- Light effects on plants
Commonwealth Scientific and Industrial Research Organisation
2010-2024
Agriculture and Food
2017-2024
Australian National University
2010
Plant Industry
2010
ACT Government
2010
Cambia
2002
University of Warsaw
2002
University of Cape Town
1998-2001
University of the Witwatersrand
1992
Abstract The microRNA159 (miR159) family represses the conserved GAMYB-like genes that encode R2R3 MYB domain transcription factors have been implicated in gibberellin (GA) signaling anthers and germinating seeds. In Arabidopsis (Arabidopsis thaliana), two major miR159 members, miR159a miR159b, are functionally specific for genes, MYB33 MYB65. These shown to be involved anther development, but there differing reports about their role promotion of flowering little is known function seed...
Abstract The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 Sr61 , with both genes independently introduced as alien chromosome introgressions tall grass ( Thinopyrum ponticum ). Mutational genomics targeted exome capture identify separate single that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. are each validated by transgenic complementation...
Fungal pathogens are a major constraint to global crop production; hence, plant genes encoding pathogen resistance important tools for combating disease. A few identified date provide partial, durable multiple and the wheat (Triticum aestivum) Lr67 hexose transporter variant (Lr67res) fits into this category. Two amino acids differ between wild-type resistant alleles - G144R V387L. Exome sequence data from 267 barley (Hordeum vulgare) landraces wild accessions was screened neither of Lr67res...
The ability of the wheat Lr34 multipathogen resistance gene (Lr34res) to function across a wide taxonomic boundary was investigated in transgenic Sorghum bicolor. Increased sorghum rust and anthracnose disease symptoms following infection with biotrophic pathogen Puccinia purpurea hemibiotroph Colletotrichum sublineolum, respectively, occurred plants expressing Lr34res ABC transporter. Transgenic lines that highly expressed exhibited immunity compared low-expressing single copy genotype...
Abstract The induced dwarf mutant Rht12 was previously shown to have agronomic potential replace the conventional DELLA mutants Rht-B1b/Rht-D1b in wheat. dwarfing gene is not associated with reduced coleoptile length (unlike mutants) and it dominant, characteristics which are shared characterized genes Rht18 Rht14. Using Rht18/Rht14 model, a gibberellin (GA) 2-oxidase identified region on chromosome 5A. A screen for suppressor background tall overgrowth individuals that were contain...
Recombination between divergent virus genomes is believed to be a major mechanism for generation of novel genotypes. We have examined the recombination process in geminiviruses by forcing two distinct isolates Maize streak (MSV), MSV-Kom and MSV-Set. Heterodimeric agroinfectious constructs containing tandemly cloned mixtures complete or partial MSV-Set were used simulate circular dimeric form similar that which would expected occur following single intermolecular crossing-over event...
Summary Plant chromosomes terminate in telomeres as other eukaryotes. Telomeres are vital to genome stability and their malfunctioning is lethal. One of the core components telomere complex telomerase. The enzyme activity depends on RNA (TER) reverse transcriptase (TERT) subunits. We describe here isolation, sequencing characterization telomerase catalytic subunit from monocot plant Oryza sativa L. ( OsTERT ). A single copy this gene present rice genome. protein predicted sequence has all...
The main cis-acting control regions for replication of the single-stranded DNA genome maize streak virus (MSV) are believed to reside within an approximately 310 nt long intergenic region (LIR). However, neither minimum LIR sequence required nor determinants specificity have been determined experimentally. There iterated sequences, or iterons, both conserved inverted-repeat sequences with potential form a stem-loop structure at origin virion-strand replication, and upstream rep gene TATA box...
Partial resistance to multiple biotrophic fungal pathogens in wheat (Triticum aestivum L.) is conferred by a variant of the Lr67 gene, which encodes hexose-proton symporter. Two mutations (G144R and V387L) differentiate resistant susceptible protein variants (Lr67res Lr67sus). Lr67res lacks sugar transport capability was associated with anion transporter-like properties when expressed Xenopus laevis oocytes. Here, we extended this functional characterization include yeast planta studies. The...
Geminiviruses, named for their unique geminate capsid morphology, have small single-stranded (ss) circular DNA genomes that replicate in the nuclei of infected cells via a double stranded (ds) intermediate. They are responsible economically devastating diseases wide variety crop species from cereals to legumes; it is thus important gain better understanding epidemiology, genetic diversity, and molecular mechanisms replication pathogenicity, design effective resistance strategies (for...
Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is a serious threat to canola (Brassica napus) production worldwide. Quantitative resistance this disease highly desirable trait but difficult precisely phenotype. Visual scores can be subjective and are prone assessor bias. Methods assess variation in quantitative more accurately were developed based on quantifying planta biomass, including Wheat Germ Agglutinin Chitin Assay (WAC), qPCR ddPCR assays.Disease assays...
Crop diseases are a significant constraint to agricultural production globally. Plant disease phenotyping is crucial for the identification, development, and deployment of effective breeding strategies, but methodologies have not kept pace with rapid progress in genetic genomic characterization hosts pathogens, still largely relying on visual assessment by trained experts. Remote sensing technologies were used develop an automatic framework extracting stems individual plants from RGB images...
Partial resistance to multiple biotrophic fungal pathogens in wheat (Triticum aestivum L.) is conferred by the Lr67 gene, which encodes a Sugar Transport Protein 13 (STP13) family hexose-proton symporter variant. Two mutations (G144R, V387L) differentiate resistant and susceptible protein variants (Lr67res Lr67sus). The molecular function of Lr67res not well understood. We functionally characterized variant using two heterologous expression systems - Xenopus laevis oocytes Saccharomyces...