Willem H. P. Boshoff

ORCID: 0000-0003-2769-5546
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About
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Research Areas
  • Wheat and Barley Genetics and Pathology
  • Yeasts and Rust Fungi Studies
  • Plant Disease Resistance and Genetics
  • Plant Pathogens and Fungal Diseases
  • Plant Pathogens and Resistance
  • Agriculture, Plant Science, Crop Management
  • Mycotoxins in Agriculture and Food
  • Genetic Mapping and Diversity in Plants and Animals
  • Genetics and Plant Breeding
  • Plant-Microbe Interactions and Immunity
  • Bioenergy crop production and management
  • Plant pathogens and resistance mechanisms
  • Berry genetics and cultivation research
  • Crop Yield and Soil Fertility
  • Plant Pathogenic Bacteria Studies
  • Fungal Plant Pathogen Control
  • Plant tissue culture and regeneration
  • Plant Virus Research Studies
  • Turfgrass Adaptation and Management
  • Insect Pest Control Strategies
  • Insect Resistance and Genetics
  • Insect behavior and control techniques
  • Powdery Mildew Fungal Diseases
  • Sunflower and Safflower Cultivation
  • Plant Micronutrient Interactions and Effects

University of the Free State
1998-2025

Chinese Academy of Sciences
2023

Institute of Genetics and Developmental Biology
2023

Génétique et biologie du développement
2023

Hebei Agricultural University
2023

Stellenbosch University
2022

Agricultural Research Council of South Africa
1997-2015

International Institute of Tropical Agriculture
1978

University of Ibadan
1978

Abstract The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 Sr61 , with both genes independently introduced as alien chromosome introgressions tall grass ( Thinopyrum ponticum ). Mutational genomics targeted exome capture identify separate single that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. are each validated by transgenic complementation...

10.1038/s41467-021-23738-0 article EN cc-by Nature Communications 2021-06-07

The role of nucleotide-binding leucine-rich repeat (NLR) receptors in plant immunity is well studied, but the function a class tandem kinases (TKs) that confer disease resistance wheat and barley remains unclear. In this study, we show SR62 locus digenic module encoding Sr62 TK an NLR (Sr62 ), identify corresponding AvrSr62 effector. binds to N-terminal kinase 1 , triggering displacement 2, which activates . Modeling mutation analysis indicated mediated by overlapping binding sites (i) on...

10.1126/science.adp5034 article EN Science 2025-03-27

Seven races have been described in the Ug99 race group of Puccinia graminis f. sp. tritici (2). Ug99-related previously recorded South Africa are TTKSF, TTKSP, and PTKST (4). In December 2010, severe stem rust infection winter wheat cv. Matlabas was observed for first time Africa. Race analysis using 20 North American (NA) differential lines letter code system classified as TTKSF. comparative studies a greenhouse, seedlings were susceptible (infection type [IT] 4) to isolate UVPgt61/1...

10.1094/pdis-12-11-1027-pdn article EN other-oa Plant Disease 2012-02-01

Common rust on maize, caused by Puccinia sorghi (Ps), is a regular occurring disease in South Africa. Knowledge the race composition, effective resistance (Rp) genes and genotypic variation among Ps isolates contributes to genetic control of our understanding pathogen diversity evolution. Ninety-six single pustule Ps, including field from 2006, 2008, 2014, 2018, 2021, 2022 production seasons, were studied. Isolates phenotyped 26 maize differential lines genotyped with 11 microsatellite...

10.1094/pdis-12-24-2585-sr article EN Plant Disease 2025-03-27

Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks., has become an endemic disease of wheat (Triticum aestivum L.) in South Africa since it was first observed near Moorreesburg, Western Cape during August 1996. The main objectives this study were to monitor the occurrence, spread, and possible development new variants stripe rust pathogen susceptibility grass species pathogen. Results surveys conducted 1996 1999 revealed that rainfed produced Cape, Eastern eastern Free...

10.1094/pdis.2002.86.5.485 article EN Plant Disease 2002-05-01

Puccinia coronata f. sp. avenae ( Pca) is an important fungal pathogen causing crown rust that impacts oat production worldwide. Genetic resistance for crop protection against Pca often overcome by the rapid virulence evolution of pathogen. This study investigated factors shaping adaptive using populations from distinct geographic regions within United States and South Africa. Phenotypic genome-wide sequencing data these diverse collections, including 217 isolates, uncovered phylogenetic...

10.1094/mpmi-09-23-0126-fi article EN cc-by-nc-nd Molecular Plant-Microbe Interactions 2023-11-13

Abstract Most plant resistance genes encode membrane-anchored receptor-like proteins or intracellular nucleotide-binding and leucine-rich repeat (NLR) receptors. In wheat barley, tandem kinases (TKs) have emerged as a new class of determinants. To understand the modus operandi stem rust protein Sr62 TK , we identified two genetic interactors— host gene required for function corresponding fungal AvrSr62 effector. We discovered that SR62 locus consists digenic module encoding an NLR (Sr62 )....

10.1101/2024.08.30.610287 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2024-08-30

The globally distributed causal agent of powdery mildew on wheat, Blumeria graminis f. sp. tritici ( Bgt ), is one the most rapidly adapting plant pathogens and requires monitoring for shifts in virulence to wheat resistance Pm ) genes. Virulence frequencies were assessed a total 346 isolates from several countries that had either lately recorded increasing epidemics (Brazil, South Africa, Australia) or not recently been surveyed (Turkey Russia). results compared previously published surveys...

10.3389/fpls.2022.954958 article EN cc-by Frontiers in Plant Science 2022-08-01

During August 1996, stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici, was observed for the first time on bread wheat (Triticum aestivum) in Western Cape, South Africa. Ensuing surveys during growing season indicated that rust occurred throughout most of wheat-producing areas winter rainfall regions Northern, Western, and Eastern Cape provinces. The disease also irrigated summer area south Kimberley. Stripe severe where prolonged cool wet conditions favored epidemic...

10.1094/pdis.1997.81.4.424d article EN Plant Disease 1997-04-01

Angular leaf spot caused by Phaeoisariopsis griseola is an economically important disease of beans ( Phaseolus vulgaris ) in southern Africa. The success local programmes breeding for resistance to this depends a large extent on the genetic variation within pathogen population. To assess variability pathogen, 28 isolates P. from various localities were compared using isozyme analysis means starch gel electrophoresis. Thirteen loci identified 10 enzyme systems. Using UPGMA, three...

10.1046/j.1365-3059.1996.d01-115.x article EN Plant Pathology 1996-04-01

Rusts are a significant threat to sustainable wheat production in South Africa. In addition accurate genotyping, resistance breeding and surveillance, better understanding of the epidemiology rust pathogens will facilitate their control. This includes an improved over-summering occurrence early-season inoculum on ancillary hosts. current study Puccinia graminis f. sp. tritici P. triticina, causing stem leaf wheat, respectively, were avirulent rye entries planted as forage crops Isolates...

10.1080/02571862.2018.1522381 article EN South African Journal of Plant and Soil 2018-12-06

Puccinia triticina (Pt) infects wheat, leading to colonization of leaf tissues, pustule formation on the and eventually yield loss. Fungicides breeding techniques are used manage Pt infection in wheat. However, there is a growing need for biochemical studies identify critical metabolites proteins that contribute plant susceptibility or resistance against Pt. The current study investigated defensive role non-structural, structural carbohydrates, CAZymes during Pt-wheat interaction at 1, 7, 14...

10.1016/j.pmpp.2023.101947 article EN cc-by-nc-nd Physiological and Molecular Plant Pathology 2023-01-05

Abstract Stem rust is a serious disease of many gramineous plants including small grain staples such as wheat, barley, rye and triticale. Separate formae speciales (ff. spp.) the causal fungus, Puccinia graminis , predominantly infect certain host plant genera. However, despite these taxonomic subdivisions, P . ff. spp. are genetically too similar to distinguish using existing genetic markers. For those infecting cereals, this particularly challenging for f. sp. tritici (Pgt) secalis (Pgs)....

10.1111/ppa.13890 article EN cc-by Plant Pathology 2024-02-29

Although crown rust (caused by Puccinia coronata f. sp. avenae) and stem graminis are generally considered to be the most widespread damaging diseases of oat (Avena spp.) in South Africa, pathogenic variability has never been studied. During 1997 1998, one dominant pathotype (SBLL) was identified with virulence resistance genes Pc40, Pc45, Pc46, Pc51, Pc54. Four other pathotypes (SGLL, PBBB+Pc35, SDQL, JBBM+Pc35), occurring at low frequencies further rendering Pc35, Pc39, Pc48, Pc50, Pc52,...

10.1094/pdis.2001.85.10.1085 article EN Plant Disease 2001-10-01
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