A5103084342- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant pathogens and resistance mechanisms
- Microbial metabolism and enzyme function
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Bacterial Genetics and Biotechnology
- Plant nutrient uptake and metabolism
- Plant Taxonomy and Phylogenetics
- Cervical Cancer and HPV Research
- Vasculitis and related conditions
- Reproductive tract infections research
- Salmonella and Campylobacter epidemiology
- Metalloenzymes and iron-sulfur proteins
- Advanced Fluorescence Microscopy Techniques
- Chromosomal and Genetic Variations
- Inflammasome and immune disorders
- Algal biology and biofuel production
- Gene expression and cancer classification
- Advanced Photonic Communication Systems
- Probiotics and Fermented Foods
- Plant Virus Research Studies
Commonwealth Scientific and Industrial Research Organisation
2021-2025
Agriculture and Food
2021-2024
ACT Government
2024
Guangdong Provincial Hospital of Traditional Chinese Medicine
2024
Beijing Center for Disease Prevention and Control
2021-2022
Second Affiliated Hospital of Guangzhou Medical University
2022
Guangzhou Medical University
2022
Institute of Entomology
2021
Guangxi Center for Disease Prevention and Control
2017
Plant Industry
2000
The promoters of MEA (FIS1), FIS2, and FIE (FIS3), genes that repress seed development in the absence pollination, were fused to beta-glucuronidase (GUS) study their activity pattern. FIS2GUS product is found embryo sac, each polar cell nuclei, central nucleus. After maternally derived protein occurs nuclei cenocytic endosperm. Before cellularization endosperm, terminated micropylar endosperm subsequently chalazal cyst. MEAGUS has a pattern similar FIS2GUS, but FIEGUS many tissues, including...
Abstract The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 Sr61 , with both genes independently introduced as alien chromosome introgressions tall grass ( Thinopyrum ponticum ). Mutational genomics targeted exome capture identify separate single that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. are each validated by transgenic complementation...
Crop breeding for durable disease resistance is challenging due to the rapid evolution of pathogen virulence. While progress in (R) gene cloning and stacking has accelerated recent years
Protein-protein interactions (PPIs) are a fundamental process in cellular biogenesis. Here we have developed split GAL4 RUBY assay that enables macroscopically visual PPI detection plant leaves real time. Candidate interacting protein partners fused to specific domains of the yeast and herpes simplex virus VP16 transcription factors transiently expressed Nicotiana benthamina by Agrobacterium infiltration. PPI, may be either direct or indirect, results transcriptional activation reporter gene...
In this study, we report a novel mcr-1 gene variant, named mcr-1.6, carried by an IncP plasmid in colistin-resistant Salmonella enterica serovar Typhimurium isolate from healthy person. Compared with mcr-1, the mcr-1.6 contains two single-nucleotide polymorphisms, one of which results arginine to histidine variation (Arg536→His). The carrying was designated pMCR1.6_P053 and is similar recently discovered mcr-1-bearing found Klebsiella pneumoniae.
The ruminant livestock industry is the largest agricultural emissions source of anthropogenic greenhouse gases, primarily from enteric methane. Several technologies, both natural and synthetic are being investigated to mitigate methane emissions. Macroalgae derived feed ingredients that contain bromoform, a recognised inhibitor methanogenesis, an effective approach for mitigation. However, producing sufficient biomass economically satisfy at global scale challenging. Here we demonstrate...
Prevention of autonomous division the egg apparatus and central cell in a female gametophyte before fertilization ensures successful reproduction flowering plants. Here we show that rice ovules Polycomb repressive complex 2 (PRC2) Osfie1 Osfie2 double mutants exhibit asexual embryo endosperm formation at high frequency, while single display pre-embryo-like structures lower frequency without fertilization. Earlier onset, higher penetrance better development embryos compared with those suggest...
Abstract Crop breeding for durable disease resistance is challenging due to the rapid evolution of pathogen virulence. While progress in ( R ) gene cloning and stacking has accelerated recent years 1-3 , identification corresponding avirulence Avr genes many pathogens hampered by lack high-throughput screening options. To address this technology gap, we developed a platform pooled library plant protoplasts interacting / pairs. We validated isolating known novel from wheat stem rust Puccinia...
Ruminant livestock produce around 24% of global anthropogenic methane emissions. Methanogenesis in the animal rumen is significantly inhibited by bromoform, which abundant seaweeds genus Asparagopsis. This has prompted development feed additives based on Asparagopsis to mitigate emissions, although this approach alone unlikely satisfy demand. Here we engineer a non-native biosynthesis pathway bromoform vivo with yeast as an alternative biological source that may enable sustainable, scalable...
Spatial transcriptomics (STs) simultaneously obtains the location and amount of gene expression within a tissue section. However, current methods like FindMarkers calculated differentially expressed genes (DEGs) based on classical statistics, which should abolish spatial information.A new method named analysis (saSpatial) was developed for both expression. Then saSpatial applied to detect DEGs in inter- intra-cross sections. detected by were compared with those FindMarkers.Spatial founded...
<p>The advent of spatially resolved transcriptomics (SRT) has revolutionized our understanding spatial gene expression patterns within tissue architecture, shifting the paradigm molecular biology and genetics. This breakthrough technology bridges gap between genomics histology, allowing for a more integrated view cellular function interaction their native context. Despite development numerous computational tools, each with its own underlying assumptions, identifying appropriate ones...
Rationale: This article presents a complex case of refractory Henoch–Schönlein purpura (HSP), initially manifesting with gastrointestinal (GI) symptoms, and discusses diagnostic therapeutic challenges encountered. It aims to enhance understanding the disease provide evidence for potential efficacy intravenous immunoglobulin (IVIG) treatment in this condition. Patient concerns: A 16-year-old male patient presented persistent abdominal pain, nausea, vomiting, constipation 8 days, leading...
Abstract Prevention of autonomous division the egg apparatus and central cell in a female gametophyte before fertilization ensures successful reproduction flowering plants. Here we show that rice ovules with PRC2 Osfie1 Osfie2 double mutations exhibit asexual embryo endosperm formation at high frequency, while single mutation display pre-embryo-like structures lower frequency without fertilization. Confocal microscopy images indicate embryos were mainly derived from eggs mutants, pre-embryos...
Objective: To aralyze the clinical characteristics of Chlamydia pneumoniae infection in Beijing. Methods: Based on Beijing Respiratory Pathogen Surveillance System, acute respiratory patients were recruited from 35 different types sentinel hospitals Their epidemiological and data systematically collected specimens also obtained. Nuclear acid testing was performed for 30 pathogens (including pneumoniae). The identified divided into two groups, upper tract (AURI) group group. differences...