A5067238824- Plant Molecular Biology Research
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Wheat and Barley Genetics and Pathology
- Genetic and Environmental Crop Studies
- Plant Physiology and Cultivation Studies
- Photosynthetic Processes and Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Bioenergy crop production and management
- Agricultural Economics and Practices
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Immunotherapy and Immune Responses
- Pneumonia and Respiratory Infections
- Legume Nitrogen Fixing Symbiosis
- Immune Response and Inflammation
- Plant Genetic and Mutation Studies
- Animal Nutrition and Physiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Soybean genetics and cultivation
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
Commonwealth Scientific and Industrial Research Organisation
2014-2025
Agriculture and Food
2017-2025
ACT Government
2012-2024
Health Sciences and Nutrition
2020-2021
Montana State University
2003-2020
Odisha University of Agriculture and Technology
2020
Biju Patnaik University of Technology
2020
Australian National University
2020
Ferghana State University
2019
Matrix Research (United States)
2014
Abstract Cell separation is thought to involve degradation of pectin by several hydrolytic enzymes, particularly polygalacturonase (PG). Here, we characterize an activation tagging line with reduced growth and male sterility caused increased expression a PG encoded QUARTET2 (QRT2). QRT2 essential for pollen grain part small family three closely related endo-PGs in the Arabidopsis thaliana proteome, including ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1) ADPG2. Functional assays...
The flowers of major cereals are arranged on reproductive branches known as spikelets, which group together to form an inflorescence. Diversity for inflorescence architecture has been exploited during domestication increase crop yields, and genetic variation this trait potential further boost grain production. Multiple genes that regulate have identified by studying alleles modify gene activity or dosage; however, little is in wheat. Here, we show TEOSINTE BRANCHED1 (TB1) regulates bread...
Innate immune mechanisms against Pneumocystis carinii, a frequent cause of pneumonia in immunocompromised individuals, are not well understood. Using both real time polymerase chain reaction as measure organism viability and fluorescent deconvolution microscopy, we show that nonopsonic phagocytosis P. carinii by alveolar macrophages is mediated the Dectin-1 β-glucan receptor subsequent generation hydrogen peroxide involved macrophage–mediated killing carinii. The macrophage colocalized with...
Gibberellins (GAs) are tetracyclic diterpenoids that essential endogenous regulators of plant growth and development. GA levels within the regulated by a homeostatic mechanism includes changes in expression family GA-inactivating enzymes known as 2-oxidases. Ectopic pea 2-oxidase2 cDNA caused seed abortion Arabidopsis, extending confirming previous observations obtained with GA-deficient mutants pea, suggesting GAs have an role A new physiological for pollen tube vivo also has been...
Abstract Gibberellin 3-oxidase (GA3ox) catalyzes the final step in synthesis of bioactive gibberellins (GAs). We examined expression patterns all four GA3ox genes Arabidopsis thaliana by promoter–β-glucuronidase gene fusions and quantitative RT-PCR defined their physiological roles characterizing single, double, triple mutants. In developing flowers, are only expressed stamen filaments, anthers, flower receptacles. Mutant plants that lack both GA3ox1 GA3ox3 functions displayed petal defects,...
The Arabidopsis (Arabidopsis thaliana) SPINDLY (SPY) protein negatively regulates the gibberellin (GA) signaling pathway. SPY is an O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) with a protein-protein interaction domain consisting of 10 tetratricopeptide repeats (TPR). OGTs add GlcNAc monosaccharide to serine/threonine residues nuclear and cytosolic proteins. Determination molecular defects in 14 new spy alleles reveals that these mutations cluster three TPRs C-terminal catalytic...
Abstract Tillering (branching) is a major yield component and, therefore, target for improving the of crops. However, tillering regulated by complex interactions endogenous and environmental signals, knowledge required to achieve optimal tiller number through genetic agronomic means still lacking. Regulatory mechanisms may be revealed physiological molecular characterization naturally occurring induced mutants in Here we characterize reduced (tin, inhibition) mutant wheat (Triticum...
EARLY FLOWERING3 (ELF3) is a circadian clock gene that contributes to photoperiod-dependent flowering in plants, with loss-of-function mutants barley (Hordeum vulgare), legumes, and Arabidopsis thaliana early under noninductive short-day (SD) photoperiods. The elf3 mutant displays increased expression of FLOWERING LOCUS T1 (FT1); however, it remains unclear whether this the only factor responsible for phenotype. We show vegetative growth phenotypes are strongly dependent on gibberellin (GA)...
Summary Resistant starch ( RS ), a type of dietary fibre, plays an important role in human health; however, the content most modern processed starchy foods is low. Cereal starch, when structurally manipulated through modified biosynthetic pathway to greatly increase amylose content, could be food source . Transgenic studies have previously revealed requirement simultaneous down‐regulation two branching enzyme SBE ) II isoforms both located on long arm chromosome 2, namely SBEII and b ,...
The advantages of free threshing in wheat led to the selection domesticated Q allele which is now present almost all modern varieties. and pre-domestication allele, q, encode an AP2 transcription factor with conferring a character subcompact (i.e. partially compact) inflorescence (spike). We demonstrate that mutations miR172-binding site gene are sufficient increase transcript levels via reduction miRNA dependent degradation, consistent conclusion SNP miRNA-binding relative q was essential...
Crop breeding for durable disease resistance is challenging due to the rapid evolution of pathogen virulence. While progress in (R) gene cloning and stacking has accelerated recent years
The gibberellin (GA) biosynthesis mutants lh‐1 and lh‐2 have been used to examine the physiological role of GAs in pea seed development. LH protein is required for three‐step oxidation ent ‐kaurene ‐kaurenoic acid early GA pathway. allele‐specific interaction with chemical inhibitors these three steps suggests that encodes multi‐functional enzyme oxidase. Unlike mutation which reduces weight decreases survival by ∼50% compared wild‐type plants, allele has a transient effect on embryo growth...
Summary Lack of grain dormancy in cereal crops such as barley and wheat is a common problem affecting farming areas around the world, causing losses yield quality because preharvest sprouting. Control seed or has been investigated extensively using various approaches different species, including Arabidopsis cereals. However, use monocot model plant Brachypodium distachyon presents opportunities for discovery new genes related to that are not present modern commercial crops. In this work we...
The Arabidopsis gynoecium is a complex organ that facilitates fertilization, later developing into dehiscent silique protects seeds until their dispersal. Identifying genes important for development often hampered by functional redundancy. We report unequal redundancy between two closely related genes, SPATULA (SPT) and ALCATRAZ (ALC), revealing previously unknown developmental roles each. SPT known to support septum, style stigma in the flower, whereas ALC involved dehiscence zone fruit....
Abstract Gibberellins (GAs) are plant hormones with diverse roles in growth and development. SPINDLY (SPY) is one of several genes identified Arabidopsis that involved GA response it thought to encode an O-GlcNAc transferase. Genetic analysis suggests SPY negatively regulates response. To test the hypothesis acts specifically as a acting component signal transduction,spy mutants plants containing 35S:SPY construct have been examined. A detailed investigation spymutant phenotype may play role...
Summary Gibberellins (GAs) are hormones required for several aspects of plant development, including internode elongation and seed development in pea ( Pisum sativum L.). The first committed step the GA biosynthesis pathway is conversion geranylgeranyl diphosphate (GGDP) to ent ‐kaurene via copalyl (CDP). These two reactions catalyzed by cyclases synthase A (KSA) B (KSB), respectively. Previous genetic biochemical analysis GA‐responsive ls‐1 mutant suggested that levels reduced a...
In recent years exciting progress has been made in understanding how endogenous plant hormones control development.One of the fundamental questions this area research is mechanism by which presente a hormone molecule (the signal) leads to change development transduction pathway).This Update will discuss genetic analysis signal for class collectively known as GAs.More extensive reviews on various aspects GA can be found Hooley (1994), Ross and Sponsel (1995). PHYSIOLOCYThe best-characterized...
To broaden our understanding of the molecular mechanisms gibberellin (GA) action, we isolated a spindly clone (HvSPY) from barley cultivar Himalaya and tested whether HvSPY protein would modulate GA action in aleurone. The cDNA showed high sequence identity to Arabidopsis SPY along its entire length, functionally complemented spy-3 mutation. proteins relatedness with animal O-linked N-acetylglucosamine transferases (OGTs), suggesting that they may also have OGT activity. has locus distinct...
Summary The regulation of gibberellin (GA) deactivation was examined using the sin (slender) mutation in garden pea ( Pisum sativum L.). This blocks GA 20 , precursor bioactive 1 . Firstly, crosses were made to combine with biosynthesis mutations na lh i and le‐3 combination produced a novel phenotype, long (‘slender’) basal internodes extremely short (‘nana’) upper internodes. In contrast, double mutant phenotypically dwarf. causes an accumulation maturing seeds, this unaffected by since is...
Flowering and flower formation are defining features of angiosperms the control these developmental processes involves a common repertoire genes which shared among different species flowering plants. These were first identified using various homeotic time mutants Arabidopsis snapdragon, homologous have subsequently been isolated from wide range plant based on conservation protein sequence function. Using degenerate reverse‐transcriptase polymerase chain reaction, we one APETALA3 ‐like (...
Flowering is an integral developmental process in angiosperms, crucial to reproductive success and continuity of the species through time. Some angiosperms complete their life cycle within a year (annual plants), others have longer life, which characterized by generation new flowering vegetative shoots every (perennial plants). Despite differences lifespan, underlying genetics flower induction floral organ formation appears be similar among these plants. Hence, knowledge gained from study...
Abstract SPY (SPINDLY) encodes a putative O-linked N-acetyl-glucosamine transferase that is genetically defined as negatively acting component of the gibberellin (GA) signal transduction pathway. Analysis Arabidopsis plants containing aSPY::GUS reporter gene reveals thatSPY expressed throughout life plant and in most organs examined. In addition to being all where phenotypes due spy mutations have been reported, SPY::GUS root. Examination roots wild-type, spy, andgai revealed indicating GAI...