A5063111442- Immunodeficiency and Autoimmune Disorders
- Psoriasis: Treatment and Pathogenesis
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune Cell Function and Interaction
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Virus-based gene therapy research
- Tuberculosis Research and Epidemiology
- Cancer Immunotherapy and Biomarkers
- Pneumonia and Respiratory Infections
- Cystic Fibrosis Research Advances
- T-cell and B-cell Immunology
- Neonatal Respiratory Health Research
- Respiratory viral infections research
- Cytokine Signaling Pathways and Interactions
- SARS-CoV-2 and COVID-19 Research
- Phagocytosis and Immune Regulation
- Pancreatic and Hepatic Oncology Research
- Antifungal resistance and susceptibility
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- CAR-T cell therapy research
- Gut microbiota and health
Tulane University
2016-2025
Children's Hospital of Pittsburgh
2012-2021
University of Pittsburgh Medical Center
2011-2021
Richard King Mellon Foundation
2012-2021
Tula University
2018-2021
Icahn School of Medicine at Mount Sinai
2021
University of Pittsburgh
2011-2020
Universidad Mesoamericana de San Agustín
2020
Our Lady of the Lake Regional Medical Center
2020
Boston Children's Hospital
2006-2015
Bacterial pneumonia is an increasing complication of HIV infection and inversely correlates with the CD4(+) lymphocyte count. Interleukin (IL)-17 a cytokine produced principally by T cells, which induces granulopoiesis via granulocyte colony-stimulating factor (G-CSF) production CXC chemokines. We hypothesized that IL-17 receptor (IL-17R) signaling critical for G-CSF chemokine lung host defenses. To test this, we used model Klebsiella pneumoniae in mice genetically deficient IL-17R or...
Abstract Mesenchymal stem cells (MSCs) and macrophages are fundamental components of the cell niche function coordinately to regulate haematopoietic self-renewal mobilization. Recent studies indicate that mitophagy healthy mitochondrial critical survival cells, but how these processes regulated in MSCs is unknown. Here we show manage intracellular oxidative stress by targeting depolarized mitochondria plasma membrane via arrestin domain-containing protein 1-mediated microvesicles. The...
Abstract Steroid-resistant asthma comprises an important source of morbidity in patient populations. TH17 cells represent a distinct population CD4+ Th that mediate neutrophilic inflammation and are characterized by the production IL-17, IL-22, IL-6. To investigate function context Ag-induced airway inflammation, we polarized naive T from DO11.10 OVA-specific TCR-transgenic mice to TH2 or phenotype culturing conditioned medium. In addition, also tested steroid responsiveness cells. vitro,...
Gut reasons to brush your teeth Some gut conditions, such as inflammatory bowel disease (IBD), ulcerative colitis, and Crohn's (CD), are associated with imbalances in the microbe community. The causes of these intractable diseases have been difficult discern. Atarashi et al. took samples from mouths IBD CD patients inoculated extracted bacteria into germ-free mice (see Perspective by Cao). showed strong proliferation T helper 1 cells establishment oral Klebsiella species colon. can be...
Staphylococcus aureus is the most common cause of skin and soft tissue infections, rapidly emerging antibiotic-resistant strains are creating a serious public health concern. If immune-based therapies to be an alternative antibiotics, greater understanding needed protective immune response against S. infection in skin. Although neutrophil recruitment required for immunity aureus, role T cells has been suggested. Here, we used mouse model cutaneous investigate contribution host defense. We...
Interleukin (IL)-23 is a heterodimeric cytokine that shares the identical p40 subunit as IL-12 but exhibits unique p19 similar to p35. IL-12/23 p40, interferon γ (IFN-γ), and IL-17 are critical for host defense against Klebsiella pneumoniae. In vitro, K. pneumoniae–pulsed dendritic cell culture supernatants elicit T production in IL-23–dependent manner. However, importance of IL-23 during vivo pulmonary challenge unknown. We show p40–deficient mice exquisitely sensitive intrapulmonary...
Alveolar macrophages represent a first-line innate host defense mechanism for clearing inhaled Aspergillus fumigatus from the lungs, yet contradictory data exist as to which alveolar macrophage recognition receptor is critical immunity A. fumigatus. Acknowledging that cell wall contains high beta-1,3–glucan content, we questioned whether beta-glucan dectin-1 played role in this process. Monoclonal antibody, soluble receptor, and competitive carbohydrate blockage indicated inflammatory...
Heme oxygenase-1 (HO-1) confers protection against a variety of oxidant-induced cell and tissue injury. In this study, we examined whether exogenous administration HO-1 by gene transfer could also confer protection. We first demonstrated the feasibility overexpressing in lung transfer. A fragment rat cDNA clone containing entire coding region was cloned into plasmid pAC-CMVpLpA, recombinant adenoviruses Ad5-HO-1 were generated homologous recombination. Intratracheal resulted time-dependent...
We examined the effects of upregulation heme oxygenase-1 (HO-1) in steatotic rat liver models ex vivo cold ischemia/reperfusion (I/R) injury. In model ischemia/isolated perfusion, treatment genetically obese Zucker rats with HO-1 inducer cobalt protoporphyrin (CoPP) or adenoviral (Ad-HO-1) significantly improved portal venous blood flow, increased bile production, and decreased hepatocyte Unlike untreated those pretreated inhibitor zinc (ZnPP), by Western blots correlated amelioration...
T helper type 17 (TH17) cells are highly proinflammatory effector that characterized by the production of high amounts IL-17A, IL-17F, IL-21, and IL-22. Furthermore, TH17 have been associated with a number autoimmune diseases. However, it is not clear whether can also serve as effective cells. Here we show function B-cell helpers in they only induce strong proliferative response B vitro but trigger antibody class switch recombination vivo. Transfer into WT or T-cell receptor α–deficient...
Although anticapsular antibodies confer serotype-specific immunity to pneumococci, children increase their ability clear colonization before these appear, suggesting involvement of other mechanisms. We previously reported that intranasal immunization mice with pneumococci confers CD4+ T cell–dependent, antibody- and serotype-independent protection against colonization. Here we show this immunity, rather than preventing initiation carriage, accelerates clearance over several days, accompanied...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Inflammatory bowel diseases (IBDs) such as Crohn's disease and ulcerative colitis are characterized by recurrent inflammation in the gastrointestinal tract. Infiltration of CD4+ lymphocytes neutrophils is one predominant features IBD. Recently, interleukin (IL)-23 downstream T cell-derived cytokine IL-17 have been found to be elevated intestinal tissue serum IBD patients. However, role IL-17R signaling gut unknown. To examine this role, we investigated wild-type or knockout mice. Using a...
Abstract Local production of IL-17 is a significant factor in effective host defense against Gram-negative bacteria. However, the proximal events mediating elaboration by T cells remain unclear. In this study, we show vivo that intact Toll-like receptor 4 signaling lung required for induction both p19 transcript IL-23 and protein response to Klebsiella pneumoniae. Although widely considered CD4+ cell product, also demonstrate vitro CD8+ after culture medium from dendritic exposed these The...