A5053486941- Occupational and environmental lung diseases
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Air Quality and Health Impacts
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Immune cells in cancer
- Aerosol Filtration and Electrostatic Precipitation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Occupational Health and Safety Research
- Risk and Safety Analysis
- Respiratory Support and Mechanisms
- Antifungal resistance and susceptibility
- Occupational exposure and asthma
- Fungal Infections and Studies
- Tissue Engineering and Regenerative Medicine
- Autophagy in Disease and Therapy
- Plant-Microbe Interactions and Immunity
- Medical Imaging and Pathology Studies
- RNA modifications and cancer
- Chemical Safety and Risk Management
- Phagocytosis and Immune Regulation
- Neuroscience of respiration and sleep
- Adenosine and Purinergic Signaling
- Pleural and Pulmonary Diseases
National Institute for Occupational Safety and Health
2021-2024
Centers for Disease Control and Prevention
2021
University of Pittsburgh
2005-2019
Abstract Mesenchymal stem cells (MSCs) and macrophages are fundamental components of the cell niche function coordinately to regulate haematopoietic self-renewal mobilization. Recent studies indicate that mitophagy healthy mitochondrial critical survival cells, but how these processes regulated in MSCs is unknown. Here we show manage intracellular oxidative stress by targeting depolarized mitochondria plasma membrane via arrestin domain-containing protein 1-mediated microvesicles. The...
Occupational exposure to crystalline silica is a well-established occupational hazard. Once in the lung, particles can result activation of alveolar macrophages (AM), potentially leading silicosis, fibrotic lung disease. Because beginning step complicated inflammatory cascade, it necessary define particle characteristics resulting this activation. The aim research was determine effect size on macrophages. RAW 264.7 were exposed four different sizes and their measured using electron...
Background Silicosis is a complex lung disease for which no successful treatment available and therefore transplantation potential alternative. Tumor necrosis factor alpha (TNFα) plays central role in the pathogenesis of silicosis. TNFα signaling mediated by transcription factor, Nuclear Factor (NF)-κB, regulates genes controlling several physiological processes including innate immune responses, cell death, inflammation. Therefore, inhibition NF-κB activation represents therapeutic strategy...
Macrophages play a fundamental role in innate immunity and the pathogenesis of silicosis. Phagocytosis silica particles is associated with generation reactive oxygen species (ROS), secretion cytokines, such as TNF, cell death that contribute to silica-induced lung disease. In macrophages, ROS production executed primarily by activation NADPH oxidase (Phox) mitochondrial (mtROS); however, relative contribution unclear, effects on macrophage function fate are unknown. this study, we used...
In the mouse lung, Escherichia coli LPS can decrease surfactant protein-B (SFTPB) mRNA and protein concentrations. also regulates expression, synthesis, concentrations of a variety gene metabolic products that inhibit SFTPB expression. The purpose present study was to determine whether acts directly or indirectly on pulmonary epithelial cells trigger signaling pathways transcription factor CCAAT/enhancer binding (C/EBP)-β (CEBPB) is downstream inhibitory effector. To investigate mechanism...
In this study a serial multi-cyclone sampling array capable of simultaneously particles multiple size fractions, from an occupational environment, for use in vivo and vitro toxicity studies physical/chemical characterization, was developed tested. This method is improvement over current methods used to size-segregate aerosols due its simplicity ability collect sufficient masses nano- ultrafine sized analysis. evaluated chamber providing uniform atmosphere dust concentrations using...
RATIONALE: MSCs are capable of paracrine activity and we have previously demonstrated, in animal models lung fibrosis, that the secretion anti-inflammatory peptides such as IL1 receptor antagonist part mediates immune regulatory properties MSCs. However, releasing micro vesicles (exosomes) extruding their mitochondria therefore, reasoned this ability to transfer these organelle would represent important mechanism for regulation innate immunity. METHODS: To test hypothesis labeled human or...