A5051931586- Plant Virus Research Studies
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Glycosylation and Glycoproteins Research
- Plant and Fungal Interactions Research
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Carbohydrate Chemistry and Synthesis
- Plant Pathogenic Bacteria Studies
- Plant Stress Responses and Tolerance
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Mosquito-borne diseases and control
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Plant Genetic and Mutation Studies
- Toxin Mechanisms and Immunotoxins
- Greenhouse Technology and Climate Control
- CRISPR and Genetic Engineering
University of Minnesota
2016-2025
University of Minnesota System
2001-2024
Twin Cities Orthopedics
2021
Horticultural Crops Research Laboratory
2021
National Clonal Germplasm Repository
2021
University of Virginia
2012
Plant (United States)
2010
Duke University
2006
Norwich Research Park
2002
John Innes Centre
2002
Three independent recessive mutations at the SPINDLY (SPY) locus of Arabidopsis confer resistance to gibberellin (GA) biosynthesis inhibitor paclobutrazol. Relative wild type, spy mutants exhibit longer hypocotyls, leaves that are a lighter green color, increased stem elongation, early flowering, parthenocarpy, and partial male sterility. All these phenotypes also observed when wild-type plants repeatedly treated with A3 (GA3). The spy-1 allele is partially epistatic ga1-2 mutation, which...
Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These selected in a screen for germination the presence gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated NaCl tolerance. The ABA‐deficiency caused by two monogenic, recessive mutations, aba2 and aba3 , that both located on chromosome 1. showed phenotype is known to be characteristic ABA‐deficiency: reduced seed dormancy excessive water loss, leading wilty phenotype....
ABSTRACT Cellular adaptations to change often involve post-translational modifications of nuclear and cytoplasmic proteins. An example found in protists plants is the modification serine threonine residues dozens hundreds nucleocytoplasmic proteins with a single fucose (O-fucose). A O-fucosyltransferase occurs pathogen Toxoplasma gondii , social amoeba Dictyostelium higher plants, where it called Spy because mutants have spindly appearance. O-fucosylation, which required for optimal...
Gibberellins (GAs) are a major class of plant hormones that control many developmental processes, including seed development and germination, flower fruit development, flowering time. Genetic studies with Arabidopsis thaliana have identified two genes involved in GA perception or signal transduction. A semidominant mutation at the GIBBERELLIN INSENSITIVE (GAI) locus results plants resembling GA-deficient mutants but exhibiting reduced sensitivity to GA. Recessive mutations SPINDLY (SPY)...
SPINDLY (SPY) is a negative regulator of gibberellin (GA) responses; however, spy mutants exhibit various phenotypic alterations not found in GA-treated plants. Assaying for additional roles SPY revealed that are resistant to exogenously applied cytokinin. GA also repressed the effects cytokinin, suggesting there cross talk between two hormone-response pathways, which may involve function. Two alleles showing severe (spy-4) and mild (spy-3) GA-associated phenotypes exhibited similar...
SPINDLY (SPY) is a negative regulator of gibberellin signaling in Arabidopsis thaliana that also functions previously undefined pathways. The N terminus SPY contains protein-protein interaction domain consisting 10 tetratricopeptide repeats (TPRs). GIGANTEA (GI) was recovered from yeast two-hybrid screen for proteins interact with the TPR domain. GI and interacted Escherichia coli vitro pull-down assays. phenotypes spy spy-4 gi-2 plants support hypothesis pathways controlling flowering,...
A tomato (Lycopersicon esculentum) gene (GAST1) that encodes an RNA whose abundance increases > 20-fold in shoots of the GA-deficient gib1 mutant following spraying with GA3 has been characterized. An increase GAST1 levels is detectable 2 h after treatment and continue to for at least additional 10 h. Between 12 24 treatment, amount begins decline 48 level nearly equivalent water-treated control plants. Nuclear runoff analysis indicates 8 GA3, transcription increased only threefold,...
The Arabidopsis (Arabidopsis thaliana) SPINDLY (SPY) protein negatively regulates the gibberellin (GA) signaling pathway. SPY is an O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) with a protein-protein interaction domain consisting of 10 tetratricopeptide repeats (TPR). OGTs add GlcNAc monosaccharide to serine/threonine residues nuclear and cytosolic proteins. Determination molecular defects in 14 new spy alleles reveals that these mutations cluster three TPRs C-terminal catalytic...
Abstract O-linked N-acetylglucosamine (O-GlcNAc) modifications regulate the posttranslational fate of target proteins. The Arabidopsis thaliana O-GlcNAc transferase (OGT) SPINDLY (SPY) suppresses gibberellin signaling and promotes cytokinin (CK) responses by unknown mechanisms. Here, we present evidence that two closely related class I TCP transcription factors, TCP14 TCP15, act with SPY to promote CK responses. TCP15 interacted in yeast two-hybrid vitro pull-down assays were modified...
Transcription activator-like effector nucleases successfully generate a heritable tomato mutant.
The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling response to developmental and environmental cues. DELLAs also play a central role mediating cross-talk between GA other pathways via antagonistic direct interactions with key factors. However, how these crucial protein–protein can be dynamically regulated during plant development remains unclear. Here, we show that are modified the O -linked N...
Abstract Gibberellin (GA) regulates plant development primarily by triggering the degradation/deactivation of DELLA proteins. However, it remains unclear whether all GA responses are regulated DELLAs. Tomato (Solanum lycopersicum) has a single gene named PROCERA (PRO), and its recessive pro allele exhibits constitutive activity but retains responsiveness to external GA. In loss-of-function mutant proΔGRAS, examined developmental were considerably enhanced relative defect in seed desiccation...
Plants employ stomatal closure and reduced growth to avoid water deficiency damage. Reduced levels of the growth-promoting hormone gibberellin (GA) lead increased tolerance deficit, but underlying mechanism is unknown. Here, we show that tomato (Solanum lycopersicum) DELLA protein PROCERA (PRO), a negative regulator GA signaling, acts in guard cells promote reduce loss response by increasing abscisic acid (ABA) sensitivity. The loss-of-function pro mutant exhibited conductance rapid wilting...
The plant hormone indole-3-acetic acid (IAA or auxin) mediates the elongation growth of shoot tissues by promoting cell expansion. According to theory proposed in 1970s, auxin activates plasma membrane H+-ATPases (PM H+-ATPases) facilitate expansion both loosening wall through acidification and solute uptake. Mechanistically, however, this process is poorly understood. Recent findings Arabidopsis (Arabidopsis thaliana) have demonstrated that auxin-induced SMALL AUXIN UP RNA (SAUR) genes...
Abstract The Arabidopsis SECRET AGENT (SEC) and SPINDLY (SPY) proteins are similar to animal O-linked N-acetylglucosamine transferases (OGTs). OGTs catalyze the transfer of (GlcNAc) from UDP-GlcNAc Ser/Thr residues proteins. In animals, O-GlcNAcylation has been shown affect protein activity, stability, and/or localization. SEC expressed in Escherichia coli had autocatalytic OGT activity. To determine function plants, two tDNA insertional mutants were identified analyzed. Although sec mutant...
The non-eveloped bacilliform viruses are the second group of plant known to possess a genome consisting circular double-stranded DNA. We have characterized viral transcript and determined complete sequence Commelina yellow mottle virus (CoYMV), member this group. Analysis indicates that encodes single terminally-redundant genome-length plus 120 nucleotide transcript. A fraction is polyadenylated, although majortty not polyadenylated. 7489 bp in size transcribed strand contains three open...
The genome of sugarcane bacilliform virus (ScBV), a badnavirus, consists circular dsDNA. complete sequence cloned infective ScBV is reported here. 7568 bp in size and possesses number features suggesting that pararetrovirus. A tRNAMet-binding site may serve as primer for minus-strand synthesis present. plus-strand the contains three open reading frames (ORFs) which are capable encoding proteins with calculated M r values 22K, 13K 215K. 215K protein has regions similarity to RNA-binding...
Journal Article Specialized binary vector for plant transformation: expression of the Arabidopsis thaliana AHAS gene in Nicotiana tabacum Get access Neil E. Olszewski, Olszewski + Present address: Department Botany, University Minnesota, St Paul, MN 55108, USA Search other works by this author on: Oxford Academic PubMed Google Scholar Frank B. Martin, Martin 1Department Applied Statistics, MinnesotaSt Frederick M. Ausubel Nucleic Acids Research, Volume 16, Issue 22, 25 November 1988, Pages...