A5026980212- Pulmonary Hypertension Research and Treatments
- Cardiovascular Function and Risk Factors
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Cardiomyopathy and Myosin Studies
- Plant pathogens and resistance mechanisms
- Plant Molecular Biology Research
- Muscle Physiology and Disorders
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Plant Reproductive Biology
- Plant Virus Research Studies
- Agricultural pest management studies
- Plant nutrient uptake and metabolism
- Circular RNAs in diseases
- Mitochondrial Function and Pathology
- Dietary Effects on Health
- Ferroptosis and cancer prognosis
- Neurogenetic and Muscular Disorders Research
- Genetically Modified Organisms Research
- Toxin Mechanisms and Immunotoxins
- Ubiquitin and proteasome pathways
- Liver Disease and Transplantation
- Polysaccharides and Plant Cell Walls
- Telomeres, Telomerase, and Senescence
University of Minnesota System
2024-2025
University of Minnesota
2013-2024
Minneapolis Heart Institute Foundation
2010-2024
Twin Cities Orthopedics
2024
Baylor College of Medicine
2024
Lynn University
2021-2023
Minneapolis Institute of Arts
2021
University of Rochester Medical Center
2013
Leiden University Medical Center
2013
NHS Blood and Transplant
2005-2009
The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling response to developmental and environmental cues. DELLAs also play a central role mediating cross-talk between GA other pathways via antagonistic direct interactions with key factors. However, how these crucial protein–protein can be dynamically regulated during plant development remains unclear. Here, we show that are modified the O -linked N...
Abstract Facioscapulohumeral muscular dystrophy is a slowly progressive but devastating myopathy caused by loss of repression the transcription factor DUX4; however, DUX4 expression very low, and protein has not been detected directly in patient biopsies. Efforts to model mice have foundered either being too severe, or lacking muscle phenotypes. Here we show that endogenous facioscapulohumeral dystrophy-specific polyadenylation signal surprisingly inefficient, use this finding develop an...
Abstract The Arabidopsis SECRET AGENT (SEC) and SPINDLY (SPY) proteins are similar to animal O-linked N-acetylglucosamine transferases (OGTs). OGTs catalyze the transfer of (GlcNAc) from UDP-GlcNAc Ser/Thr residues proteins. In animals, O-GlcNAcylation has been shown affect protein activity, stability, and/or localization. SEC expressed in Escherichia coli had autocatalytic OGT activity. To determine function plants, two tDNA insertional mutants were identified analyzed. Although sec mutant...
<h3>Objective:</h3> Facioscapulohumeral muscular dystrophy (FSHD) is a neuromuscular disease with an unclear genetic mechanism. Most patients have contraction of the D4Z4 macrosatellite repeat array at 4qter, which thought to cause partial demethylation (FSHD1) contracted allele. Demethylation has been surveyed 3 restriction enzyme sites in first and only single site across entire array, current models postulate that generalized chromatin alteration causes FSHD. The background normal alleles...
Highlights•Widespread low-level expression of DUX4 from chrX causes male-specific lethality•Retinal leads to retinal vascular pathology•Low levels impair proliferation and differentiation primary myoblasts•Impaired regeneration by donor satellite cells provides a model for pathologySummaryFacioscapulohumeral muscular dystrophy (FSHD) is an enigmatic disease associated with epigenetic alterations in the subtelomeric heterochromatin D4Z4 macrosatellite repeat. Each repeat unit encodes DUX4,...
The hexosamine biosynthetic pathway (HBP) converts glucose to uridine-diphosphate-
Intermittent fasting (IF) extends life span via pleotropic mechanisms, but one important molecular mediator is adenosine monophosphate-activated protein kinase (AMPK). AMPK enhances lipid metabolism and modulates microtubule dynamics. Dysregulation of these pathways causes right ventricular (RV) failure in patients with pulmonary arterial hypertension. In rodent hypertension, IF activates RV AMPK, which restores mitochondrial peroxisomal morphology restructures regulation. addition,...
Mitochondrial dysfunction, characterized by impaired lipid metabolism and heightened reactive oxygen species generation, results in peroxidation ferroptosis. Ferroptosis is an inflammatory mode of cell death that promotes complement activation macrophage recruitment. In pulmonary arterial hypertension (PAH), endothelial cells exhibit cellular phenotypes promote Moreover, there ectopic deposition accumulation the vasculature. However, effects ferroptosis inhibition on these pathogenic...
Facioscapulohumeral muscular dystrophy (FSHD) is caused by inappropriate expression of the double homeodomain protein DUX4. DUX4 has bimodal effects, inhibiting myogenic differentiation and blocking MyoD at low levels expression, killing myoblasts high levels. Pax3 Pax7, which contain related homeodomains, antagonize cell death phenotype in C2C12 cells, suggesting some type competitive interaction. Here, we show that effects on require homeodomains but do not C-terminal activation domain We...
Right ventricular dysfunction (RVD) is a risk factor for death in multiple cardiovascular diseases, but RV-enhancing therapies are lacking. Inhibition of glycoprotein-130 (GP130) signaling with the small molecule SC144 improves RV function rodent RVD via anti-inflammatory and metabolic mechanisms. However, SC144's efficacy molecular effects translational large animal model unknown. 4-week-old castrated male pigs underwent pulmonary artery banding (PAB). After 3 weeks, PAB were randomized...
Abstract The female-predominate sex hormone 17β-estradiol exerts cardioprotective effects via multiple mechanisms. Available data demonstrate modulates microtubule dynamics in vitro , but its on pathogenic remodeling pressure-overloaded cardiomyocytes are unexplored. Here, we show directly blunts polymerization counteracts endothelin-mediated iPSC-cardiomyocytes, and mitigates stabilization pulmonary artery banded right ventricular cardiomyocytes. treatment cardiomyocyte nuclear hypertrophy,...
Background: Right atrial (RA) dysfunction is an emerging risk factor for poor outcomes in pulmonary arterial hypertension, however the mechanisms underlying compromised RA function are understudied. Objectives: Multi-omic analyses defined cellular and molecular mediators associated with artery banded (PAB) swine. Methods: 4-week-old castrated male Yorkshire pigs were subjected to PAB aged six weeks induce right heart failure. Cardiac MRI evaluated size function. snRNAseq cell-specific...
Serine and threonine of many nuclear cytoplasmic proteins are posttranslationally modified with O-linked N-acetylglucosamine (O-GlcNAc). This modification is made by transferases (OGTs). Genetic biochemical data have demonstrated the existence two OGTs Arabidopsis thaliana, SECRET AGENT (SEC) SPINDLY (SPY), at least partly overlapping functions, but there little information on their target proteins. The N terminus capsid protein (CP) Plum pox virus (PPV) isolated from Nicotiana clevelandii...
O-GlcNAc transferase (OGT) catalyses transfer of GlcNAc (N-acetylglucosamine) to serine or threonine proteins. The Arabidopsis OGTs, SECRET AGENT (SEC) and SPINDLY (SPY) have overlapping functions during gametogenesis embryogenesis. SPY in a number processes including circadian, light, gibberellin (GA) responses. role SEC plant development GA signalling was investigated by determining the phenotypes sec-1 sec-2 plants expression pattern SEC. Similar SPY, transcripts were ubiquitous. Although...
Right ventricular dysfunction (RVD) is the leading cause of death in pulmonary arterial hypertension (PAH), but no RV-specific therapy exists. We showed microtubule-mediated junctophilin-2 dysregulation (MT-JPH2 pathway) causes t-tubule disruption and RVD rodent PAH, druggable regulators this critical pathway are unknown. GP130 (glycoprotein 130) activation induces cardiomyocyte microtubule remodeling vitro; however, effects signaling on MT-JPH2 resulting from PAH undefined.
It is important to know if forage yields of alfalfa ( Medicago sativa L.) cultivars could be improved by using a subset genetically diverse parents selected from larger population for synthetic cuitivar. We used restriction fragment length polymorphisms assess the level genetic diversity among 93 genotypes previously as potential commercial On basis average pairwise distances between genotypes, four populations (samples) were developed with 2, 4, 8, 12, 16, or 24 dissimilarity (DIS)...
Thirteen Phaseolus vulgaris L. genetic stocks were developed and released by the Wisconsin Agricultural Experiment Station University of Wisconsin: SARC1 (Reg. no. GS-10, PI 628619), SARC2 GS-11, 628620), SARC3 GS-12, 628621), SARC4 GS-13, 628622), PARC1 GS-14, PI628623), PARC2 GS-15, PI628624), PARC3 GS-16, 628625), PARC4 GS-17, 628626), SMARC1-PN1 GS-18, 628627), SMARC2-PN1 GS-19, 628628), SMARC4-PN1 GS-20, 628629), SMARC1N-PN1 GS-21, 628630), L12-56 GS-22, 628631). The resistance some...
Four variants of arcelin, an insecticidal seed storage protein bean, Phaseolus vulgaris L., were investigated. Each variant (arcelin-1, -2, -3, and -4) was purified, solubilities M rs determined. For arcelins-1, -4, the isoelectric points, hemagglutinating activities, immunological cross-reactivities, N-terminal amino acid sequences On basis native denatured rs, classified as being composed dimer (arcelin-2), tetramer (arcelins-3 -4), or both proteins (arcelin-1). Although (arcelins-1d -2)...