A5101444695- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Organ Transplantation Techniques and Outcomes
- Immune Cell Function and Interaction
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Renal Transplantation Outcomes and Treatments
- Asthma and respiratory diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- IL-33, ST2, and ILC Pathways
- Adipokines, Inflammation, and Metabolic Diseases
- Single-cell and spatial transcriptomics
- Xenotransplantation and immune response
- Liver Disease and Transplantation
- Transplantation: Methods and Outcomes
- Inflammation biomarkers and pathways
- Medical Imaging and Pathology Studies
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
- Phagocytosis and Immune Regulation
University of Minnesota
2020-2025
Integra (United States)
2025
University of Minnesota Medical Center
2020-2024
University of Minnesota System
2023
Williams (United States)
2016-2023
Health Net
2023
Oregon Health & Science University
2023
Washington University in St. Louis
2014-2021
The Ohio State University
1998-2021
Institute of Immunology
2021
A prospective evaluation for possible placenta accreta was performed in 34 patients with previa and a history of one or more cesarean sections. Sonographic criteria used included (1) loss the normal hypoechoic retroplacental myometrial zone, (2) thinning disruption hyperechoic uterine serosa-bladder interface, (3) presence focal exophytic masses. Of 18 positive sonographic results, 14 had proof 16 underwent hysterectomy. negative only accreta, two required Presence numerous intraplacental...
Rationale: Monocyte infiltration into the subintimal space and its intracellular lipid accumulation are most prominent features of atherosclerosis. To understand pathophysiology atherosclerotic disease, we need to characteristics lipid-laden foamy macrophages in during Objective: We sought examine transcriptomic profiles nonfoamy isolated from intima. Methods Results: Single-cell RNA sequencing analysis CD45 + leukocytes murine aorta revealed that there macrophage subpopulations with...
Abstract Atherosclerosis is driven by the expansion of cholesterol-loaded ‘foamy’ macrophages in arterial intima. Factors regulating foamy macrophage differentiation and survival plaque remain poorly understood. Here we show, using trajectory analysis integrated single-cell RNA sequencing data a genome-wide CRISPR screen, that triggering receptor expressed on myeloid cells 2 (Trem2) associated with specification. Loss Trem2 led to reduced ability take up oxidized low-density lipoprotein...
The transcription factor Gata6 regulates proliferation and differentiation of epithelial endocrine cells cancers. Among hematopoietic cells, is expressed selectively in resident peritoneal macrophages. We thus examined whether the loss macrophage compartment affected macrophages, using Lyz2-Cre x Gata6flox/flox mice to tackle this issue. In mice, compartment, but not macrophages other organs, was contracted, with only a third normal number remaining. Heightened rates death explained marked...
Peritoneal and pleural resident macrophages in the mouse share common features each compartment exist as two distinct subpopulations: F4/80(+) MHC II(+) CD11c(+) macrophages. derive from embryonic precursors, their maintenance is controlled by Gata6. However, origin regulatory factors that maintain remain unknown. Here, we show arise postnatally CCR2-dependent precursors resemble monocytes. Monocytes continuously replenish this subset through adulthood. Gene expression analysis identified...
Current models of B lymphocyte biology posit that cells continuously recirculate between lymphoid organs, without accumulating in peripheral healthy tissues. Nevertheless, lymphocytes are one the most prevalent leukocyte populations naive murine heart. To investigate this apparent inconsistency literature, we conducted a systematic analysis myocardial cell ontogeny, trafficking dynamics, histology, and gene expression patterns. We found represent subpopulation circulating make close contact...
Abstract While macrophages are among the most abundant immune cell type found within primary and metastatic mammary tumors, how their complexity heterogeneity change with progression remains unknown. Here, were isolated from lungs of mice bearing orthotopic tumors for single-cell RNA sequencing (scRNA-seq). Seven distinct macrophage clusters identified, including populations exhibiting enhanced differential expression genes related to antigen presentation (H2-Aa, Cd74), cycle (Stmn1, Cdk1),...
BACKGROUND: Trem2 (triggering receptor on myeloid cells 2), a surface lipid receptor, is expressed foamy macrophages within atherosclerotic lesions and regulates cell survival, proliferation, anti-inflammatory responses. Studies examining the role of in atherosclerosis have shown that deletion leads to impaired macrophage uptake, cholesterol efflux. Thus, we tested hypothesis administration agonist antibody (AL002a) atherogenic mice would enhance survival decrease necrotic core formation...
Mitochondrial dysfunction, characterized by impaired lipid metabolism and heightened reactive oxygen species generation, results in peroxidation ferroptosis. Ferroptosis is an inflammatory mode of cell death that promotes complement activation macrophage recruitment. In pulmonary arterial hypertension (PAH), endothelial cells exhibit cellular phenotypes promote Moreover, there ectopic deposition accumulation the vasculature. However, effects ferroptosis inhibition on these pathogenic...
Our aim was to study the impact of subclinical inflammation on development interstitial fibrosis and tubular atrophy (IF/TA) a 1-year protocol biopsy in patients rapid steroid withdrawal (RSW). A total 256 were classified based findings at months 1 or 4. Group is 172 with no inflammation, group 2 50 (SCI), 3 19 acute rejection (SAR) 4 15 clinical (CAR). On biopsy, more (SCI) (34%, p = 0.004) (53%, 0.0002), had an IF/TA score > compared (control) (15%). not increased (CAR) (20%). The percent...
Atopic asthma is a chronic inflammatory disease of the lungs generally marked by excessive Th2 inflammation. The role allergen-specific IgG in still controversial; however, receptor IgG-immune complexes (IgG-ICs), FcγRIII, has been shown to promote responses through an unknown mechanism. Herein, we demonstrate that IgG-ICs, formed upon reexposure allergen, promoted two different models IC-mediated inflammation were independent preformed T cell memory response. Development Th2-type airway was...
Lymphatic collecting vessels direct lymph into and from nodes (LNs) can become hyperpermeable as the result of a previous infection. Enhanced permeability has been implicated in compromised immunity due to reduced flow immune cells LNs, which are primary site antigen presentation T cells. Presently, very little is known about molecular signals that affect lymphatic vessel permeability. Here, we have shown controlled by CCR7 chronic hyperpermeability observed Ccr7–/– mice followed fibrosis....
Two resident macrophage subsets reside in peritoneal fluid. Macrophages also within mesothelial membranes lining the cavity, but they remain poorly characterized. Here, we identified two populations (LYVE1hi MHC IIlo-hi CX3CR1gfplo/− and LYVE1lo/− IIhi CX3CR1gfphi subsets) mesenteric parietal linings of peritoneum. These macrophages resembled LYVE1+ surface numerous organs. Fate-mapping approaches analysis newborn mice showed that LYVE1hi predominantly originated from embryonic-derived...