A5004043546- Atherosclerosis and Cardiovascular Diseases
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Paraoxonase enzyme and polymorphisms
- Nuts composition and effects
- Cell Adhesion Molecules Research
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Coenzyme Q10 studies and effects
- Biochemical Acid Research Studies
- Cardiovascular Disease and Adiposity
- Cardiac Fibrosis and Remodeling
- Adipokines, Inflammation, and Metabolic Diseases
- IL-33, ST2, and ILC Pathways
- Antioxidant Activity and Oxidative Stress
- Vasculitis and related conditions
- Cancer-related molecular mechanisms research
- Lipid metabolism and biosynthesis
- Medical Imaging Techniques and Applications
- Nuclear Receptors and Signaling
- Macrophage Migration Inhibitory Factor
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Muscle metabolism and nutrition
- Cynara cardunculus studies
- Steroid Chemistry and Biochemistry
University of Cambridge
2015-2025
British Heart Foundation
2025
Universidad Complutense de Madrid
2004-2021
Instituto de Salud Carlos III
2011-2021
Centro de Investigación en Red en Enfermedades Cardiovasculares
2020-2021
Centro de Investigación Biomédica en Red
2021
Addenbrooke's Hospital
2019
CeMM Research Center for Molecular Medicine
2017
Austrian Academy of Sciences
2017
Paris Cardiovascular Research Center
2017
Assessing inflammatory disease activity in large vessel vasculitis (LVV) can be challenging by conventional measures. We aimed to investigate somatostatin receptor 2 (SST2) as a novel inflammation-specific molecular imaging target LVV. In prospective, observational cohort study, vivo arterial SST2 expression was assessed positron emission tomography/magnetic resonance (PET/MRI) using 68Ga-DOTATATE and 18F-FET-βAG-TOCA. Ex mapping of the performed immunofluorescence microscopy; mass...
Abstract Type-2 innate lymphoid cells (ILC2) are a prominent source of type II cytokines and found constitutively at mucosal surfaces in visceral adipose tissue. Despite their role limiting obesity, how ILC2s respond to high fat feeding is poorly understood, direct influence on the development atherosclerosis has not been explored. Here, we show that ILC2 present para-aortic tissue lymph nodes display an inflammatory-like phenotype atypical resident ILC2. High alters both number cytokine...
Abdominal aortic aneurysm (AAA) is increasing due to aging of the population and a major cause death among elderly. Ultrasound screening programs are useful in early diagnosis, but size not always good predictor rupture. Our aim was analyze value circulating molecules related oxidative stress inflammation as new biomarkers assist management AAA. The markers were quantified by ELISA, their expression aneurysmal wall studied real-time PCR immunostaining. Correlation analysis with diameter peak...
The adaptive immune response plays an important role in atherosclerosis. In to a high-fat/high-cholesterol (HF/HC) diet, marginal zone B (MZB) cells activate atheroprotective programme by regulating the differentiation and accumulation of 'poorly differentiated' T follicular helper (Tfh) cells. On other hand, Tfh germinal centre response, which promotes atherosclerosis through production class-switched high-affinity antibodies. We therefore investigated direct IL18
Calcific aortic valve disease is similar to atherosclerosis in that both diseases result from chronic inflammation and endothelial dysfunction. Heterozygous NOTCH1 mutations have been associated calcific a bicuspid valve. We investigated whether mice with genetic inactivation of the Notch signaling pathway are prone develop when exposed predisposing diet.Using Doppler echocardiography, histology, immunohistochemistry, quantitative gene expression analysis, cell culture assays, we examined...
To determine the role of NOTCH during arterial injury response and subsequent chronic arterial-wall inflammation underlying atherosclerosis.We have generated a mouse model endothelial-specific (Cdh5-driven) depletion Notch effector recombination signal binding protein for immunoglobulin kappa J region (RBPJ) [(ApoE-/-); homozygous RBPJk conditional mice (RBPJflox/flox); Cadherin 5-CreERT, tamoxifen inducible driver (Cdh5-CreERT)]. Endothelial-specific deletion RBPJ or systemic Notch1 in...
Objective— To determine the role of regulatory B cell–derived interleukin (IL)-10 in atherosclerosis. Approach and Results— We created chimeric Ldlr −/− mice with a cell–specific deficiency IL-10, confirmed that purified cells stimulated lipopolysaccharide failed to produce IL-10 compared control chimeras. Mice lacking B-cell demonstrated enhanced splenic numbers but no major differences subsets, T cell or monocyte distribution, unchanged body weights serum cholesterol levels mice. After 8...
NR4A orphan receptors have been well studied in vascular and myeloid cells where they play important roles the regulation of inflammation atherosclerosis. NR4A1 (nerve growth factor IB) is among most highly induced transcription factors B following BCR (B-cell receptor) stimulation. Given that substantially contribute to development atherosclerosis, we examined whether regulates B-cell function during atherogenesis. Approach Results: We found feeding Ldlr-/- mice a Western diet increased...
BACKGROUND: Atherosclerosis is a chronic inflammatory disease of the artery wall. Regulatory T cells (Tregs) limit inflammation and promote tissue healing. Low doses interleukin (IL)-2 have potential to increase Tregs, but its use contraindicated for patients with ischemic heart disease. METHODS: In this randomized, double-blind, placebo-controlled, dose-escalation trial, we tested low-dose subcutaneous aldesleukin (recombinant IL-2), given once daily 5 consecutive days. study part A,...
Innate lymphoid cells type 2 (ILC2s) play critical homeostatic functions in peripheral tissues. ILC2s reside perivascular niches and limit atherosclerosis development. also the pericardium but their role postischemic injury is unknown. We examined of ILC2 a mouse model myocardial infarction (MI), compared mice with or without genetic deletion ILC2. determined infarct size using histology heart function echocardiography. assessed cardiac flow cytometry RNA sequencing. Based on these data, we...
Background: A number of recent studies indicate that antioxidants reduce the oxidative stress associated with development coronary heart diseases (CHD).Objective: (i) To investigate whether erythrocyte catalase (CAT), superoxide dismutase (SOD), total glutathione, reduced glutathione (GSH), oxidized (GSSG), and lipid peroxidation (LPO), serum uric acid paraoxonase-1 (PON1) are modified at increased CHD-risk individuals consuming walnut-enriched meat (WM), (ii) to evaluate these changes were...
The integrin lymphocyte function–associated antigen 1 (LFA-1) helps to coordinate the migration, adhesion, and activation of T cells through interactions with intercellular adhesion molecule (ICAM-1) ICAM-2. LFA-1 is activated during engagement chemokine receptors cell receptor (TCR) inside-out signaling, a process that partially mediated by phosphoinositide 3-kinase (PI3K) its product phosphatidylinositol 3,4,5-trisphosphate (PIP 3 ). To evaluate potential roles PI3K in activation, we...
Diverse B cell responses and functions may be involved in atherosclerosis. Protective antibody responses, such as those against oxidized lipid epitopes, are thought to mainly derive from T cell-independent innate subsets. In contrast, both pathogenic protective roles have been associated with cell-dependent antibodies, their importance humans mouse models is still unclear.To specifically target production by plasma cells determine the impact on atherosclerotic plaque development mice without...
Abstract Liver X receptor-α (LXRα) regulates cellular cholesterol abundance and potently activates hepatic lipogenesis. Here we show that at least 1 in 450 people the UK Biobank carry functionally impaired mutations LXRα, which is associated with biochemical evidence of dysfunction. On a western diet, male female mice homozygous for dominant negative mutation LXRα have elevated liver cholesterol, diffuse crystal accumulation develop severe hepatitis fibrosis, despite reduced triglyceride no...
High postprandial lipaemia increases cardiovascular risk. Algae consumption may affect lipoproteinaemia. The effects of dietary alga and cholesterol supplementation on lipoproteinaemia arylesterase (AE) activity in growing male Wistar rats were tested the present study. Six groups ten fed a casein-based diet for 3 weeks. Three diets contained 2·4 % cholesterol-raising agent (Chol), while other three did not (NChol). Seven percentage control (NChol-C Chol-C) consisted cellulose–wheat starch...
Abstract Aims While acute cardiovascular complications of coronavirus disease 2019 (COVID-19) are well described, less is known about longer-term cardiac sequelae. For many individuals with signs or symptoms arising after COVID-19 infection, the aetiology remains unclear. We examined immune profiles associated magnetic resonance imaging (MRI) abnormalities in patients unexplained injury COVID-19. Methods and results Twenty-one participants {mean age 47 [standard deviation (SD) 13] years, 71%...