A5046594613- Atherosclerosis and Cardiovascular Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Lipoproteins and Cardiovascular Health
- Cardiac Fibrosis and Remodeling
- Cardiovascular Disease and Adiposity
- Adipose Tissue and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Acute Myeloid Leukemia Research
- Cardiac Imaging and Diagnostics
- Adenosine and Purinergic Signaling
- Single-cell and spatial transcriptomics
- Acute Myocardial Infarction Research
- Cardiac Valve Diseases and Treatments
- Coronary Interventions and Diagnostics
- Cancer, Lipids, and Metabolism
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Venous Thromboembolism Diagnosis and Management
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac electrophysiology and arrhythmias
- Inflammasome and immune disorders
University of Freiburg
2016-2025
Universitäts-Herzzentrum Freiburg-Bad Krozingen
2016-2025
University Medical Center
2025
University Medical Center Freiburg
2008-2024
University Medical Center Hamburg-Eppendorf
2016-2020
Ludwig-Maximilians-Universität München
2019
Institute for Sports Medicine
2019
La Jolla Institute for Immunology
2014-2018
Friedrich-Alexander-Universität Erlangen-Nürnberg
2018
Center for Systems Biology
2012-2016
Healing after myocardial infarction involves the biphasic accumulation of inflammatory lymphocyte antigen 6C (Ly-6C)(high) and reparative Ly-6C(low) monocytes/macrophages (Mo/MΦ). According to 1 model, Mo/MΦ heterogeneity in heart originates blood sequential recruitment distinct monocyte subsets that differentiate macrophages. Alternatively, may arise tissue from circulating subset via local macrophage differentiation polarization. The orphan nuclear hormone receptor, receptor subfamily 4,...
Atherosclerosis is a chronic inflammatory disease that driven by the interplay of pro- and anti-inflammatory leukocytes in aorta. Yet, phenotypic transcriptional diversity aortic poorly understood.We characterized from healthy atherosclerotic mouse aortas in-depth single-cell RNA-sequencing mass cytometry (cytometry time flight) to define an atlas immune cell landscape atherosclerosis.Using chow diet- Western diet-fed Apoe-/- Ldlr-/- mice, we detected 11 principal leukocyte clusters with...
Recognition and clearance of a bacterial infection are fundamental properties innate immunity. Here, we describe an effector B cell population that protects against microbial sepsis. Innate response activator (IRA) cells phenotypically functionally distinct, develop diverge from B1a cells, depend on pattern-recognition receptors, produce granulocyte-macrophage colony-stimulating factor. Specific deletion IRA activity impairs clearance, elicits cytokine storm, precipitates septic shock. These...
The adult mammalian heart has limited endogenous regenerative capacity and heals through the activation of inflammatory fibrogenic cascades that ultimately result in formation a scar. After infarction, massive cardiomyocyte death releases broad range damage-associated molecular patterns initiate both myocardial systemic responses. TLRs (toll-like receptors) NLRs (NOD-like recognize (DAMPs) transduce downstream proinflammatory signals, leading to upregulation cytokines (such as interleukin-1,...
BACKGROUND: Immune checkpoint inhibitors (ICIs), though revolutionary in cancer treatment, may accelerate atherosclerosis by inducing arterial inflammation. Due to a lack of controlled studies, the capacity 2-deoxy-2-[ 18 F]fluoro-D-glucose ([ F]FDG) uptake patients with detect this inflammation remains unclear. METHODS: Arterial [ F]FDG at 6 anatomic landmarks was assessed on serial positron emission tomography scans treated German University Hospital between January 2010 and May 2023....
Pneumonia is a major cause of mortality worldwide and serious problem in critical care medicine, but the immunophysiological processes that confer either protection or morbidity are not completely understood. We show response to lung infection, B1a B cells migrate from pleural space parenchyma secrete polyreactive emergency immunoglobulin M (IgM). The process requires innate activator (IRA) cells, transitional B1a-derived inflammatory subset which controls IgM production via autocrine...
Atherosclerotic lesions grow via the accumulation of leukocytes and oxidized lipoproteins in vessel wall. Leukocytes can attenuate or augment atherosclerosis through release cytokines, chemokines, other mediators. Deciphering how develop, oppose, complement each other's function shape course disease illuminate our understanding atherosclerosis. Innate response activator (IRA) B cells are a recently described population granulocyte macrophage colony-stimulating factor-secreting hitherto...
Throughout the inflammatory response that accompanies atherosclerosis, autoreactive CD4+ T-helper cells accumulate in atherosclerotic plaque. Apolipoprotein B100 (apoB), core protein of low-density lipoprotein, is an autoantigen drives generation pathogenic type 1 (TH1) with proinflammatory cytokine secretion. Clinical data suggest existence apoB-specific T atheroprotective, regulatory cell (Treg) phenotype healthy individuals. Yet, function apoB-reactive Tregs and their relationship TH1...
Background: Platelets store large amounts of serotonin that they release during thrombus formation or acute inflammation. This facilitates hemostasis and modulates the inflammatory response. Methods: Infarct size, heart function, cell composition were analyzed in mouse models myocardial reperfusion injury with genetic pharmacological depletion platelet serotonin. These studies complemented by vitro stimulation assays platelets leukocytes mice men, measuring plasma levels leukocyte activation...
Extracellular adenosine triphosphate (ATP) binds as a danger signal to purinergic receptor P2X7 and promotes inflammasome assembly interleukin-1β expression. We hypothesized functional role of the axis ATP-P2X7 in activation chronic inflammation driving atherosclerosis.P2X7-competent P2X7-deficient macrophages were isolated stimulated with lipopolysaccharide, ATP, or both. To assess whether may have atherosclerosis, expression was analyzed aortic arches from low density lipoprotein...
Abstract Integrin-based therapeutics have garnered considerable interest in the medical treatment of inflammation. Integrins mediate fast recruitment monocytes and neutrophils to site inflammation, but are also required for host defense, limiting their therapeutic use. Here, we report a novel monoclonal antibody, anti-M7, that specifically blocks interaction integrin Mac-1 with its pro-inflammatory ligand CD40L, while not interfering alternative ligands. Anti-M7 selectively reduces leukocyte...
Abstract Aims Mental stress substantially contributes to the initiation and progression of human disease, including cardiovascular conditions. We aim investigate underlying mechanisms these contributions since they remain largely unclear. Methods results Here, we show in humans mice that leucocytes deplete rapidly from blood after a single episode acute mental stress. Using cell-tracking experiments animal models stress, found exposure leads prompt uptake inflammatory distinct tissues heart,...
Similar to the brain, eye is considered an immune-privileged organ where tissue-resident macrophages provide major immune cell constituents.However, little known about spatially restricted macrophage subsets within different compartments with regard their origin, function, and fate during health disease.Here, we combined single-cell analysis, mapping, parabiosis, computational modeling comprehensively examine myeloid in distinct parts of homeostasis.This approach allowed us identify...
Rationale: CD40L figures prominently in chronic inflammatory diseases such as atherosclerosis. However, since potently regulates immune function and hemostasis by interaction with CD40 receptor the platelet integrin GPIIb/IIIa, its global inhibition compromises host defense generated thromboembolic complications clinical trials. We recently reported that mediates atherogenesis independently of proposed Mac-1 an alternate receptor. Objective: Here, we molecularly characterized CD40L-Mac-1...
Background— Adaptive neovascularization after arterial occlusion is an important compensatory mechanism in cardiovascular disease and includes both the remodeling of pre-existing vessels to collateral arteries (arteriogenesis) angiogenic capillary growth. We now aimed identify regulatory microRNAs involved modulation femoral artery mice. Methods Results— Using microRNA-transcriptome analysis, we identified miR-155 as a downregulated microRNA during hindlimb ischemia. Correspondingly,...
Objective— A solid body of evidence supports a role extracellular ATP and its P2 receptors in innate adaptive immunity. It promotes inflammation as danger signal various chronic inflammatory diseases. Thus, we hypothesize contribution receptor P2Y 2 vascular atherosclerosis. Approach Results— Extracellular induced leukocyte rolling, adhesion, migration vivo assessed by intravital microscopy sterile peritonitis. To test the atherosclerosis, or saline control was injected intraperitoneally 3×...
Abstract Diabetes worsens atherosclerosis progression and leads to a defect in repair of arteries after cholesterol reduction, process termed regression. Empagliflozin reduces blood glucose levels via inhibition the sodium cotransporter 2 (SGLT-2) kidney has been shown lead marked reduction cardiovascular events humans. To determine whether lowering by empagliflozin accelerates regression mouse model, male C57BL/6J mice were treated intraperitoneally with LDLR- SRB1- antisense...
Abstract Myeloid cells such as resident retinal microglia (MG) or infiltrating blood‐derived macrophages (Mϕ) accumulate in areas of ischemia and neovascularization (RNV) modulate neovascular eye disease. Their temporospatial distribution biological function this process, however, remain unclarified. Using state‐of‐the‐art methods, including cell‐specific reporter mice high‐throughput RNA sequencing (RNA Seq), study determined the extent MG proliferation Mϕ infiltration with RNV Cx3cr1...