Hansjürgen Agostini
A5046568462- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Glaucoma and retinal disorders
- Polyomavirus and related diseases
- Retinal and Macular Surgery
- Retinal Development and Disorders
- Full-Duplex Wireless Communications
- Ocular Diseases and Behçet’s Syndrome
- Angiogenesis and VEGF in Cancer
- Retinopathy of Prematurity Studies
- Intraocular Surgery and Lenses
- Plant Virus Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Antenna Design and Analysis
- Traumatic Ocular and Foreign Body Injuries
- interferon and immune responses
- Immune cells in cancer
- Facial Trauma and Fracture Management
- Cancer, Hypoxia, and Metabolism
- Vascular Malformations Diagnosis and Treatment
- Viral-associated cancers and disorders
- Corneal surgery and disorders
- Protease and Inhibitor Mechanisms
- Axon Guidance and Neuronal Signaling
University of Freiburg
2016-2025
University Medical Center Freiburg
2016-2025
Ophthalmology Clinic
2016-2025
Augenklinik Universitätsklinikum Würzburg
2019-2024
Eye Center
2017-2024
University of Sheffield
2024
University of Bonn
2024
Bicêtre Hospital
2015-2019
University Hospital of Basel
2017
Universität Hamburg
2017
PurposeTo evaluate 2-year efficacy, durability, and safety of the bispecific antibody faricimab, which inhibits both angiopoietin-2 VEGF-A.DesignTENAYA (ClinicalTrials.gov identifier, NCT03823287) LUCERNE NCT03823300) were identically designed, randomized, double-masked, active comparator-controlled phase 3 noninferiority trials.ParticipantsTreatment-naive patients with neovascular age-related macular degeneration (nAMD) 50 years age or older.MethodsPatients randomized (1:1) to intravitreal...
The human polyomavirus JC (JCV) causes the central demyelinating disease progressive multifocal leukoencephalopathy in about 5% of AIDS patients. To characterize type profile JCV a control population United States, 54 females (10 to 79 years age; average age, 43.4 years) and 51 males (18 94 47.9 were examined for excretion different genotypes their urine by PCR followed direct cycle sequencing. group consisted 89 patients general medical clinic addition 16 healthy volunteers. overall...
The human polyomavirus JC (JCV) causes the central nervous system demyelinating disease progressive multifocal leukoencephalopathy. Previously, we showed that 40% of Caucasians in United States excrete JCV urine as detected by PCR. We have now studied 68 Navaho from New Mexico, 25 Flathead Montana, and 29 Chamorro Guam. By using PCR amplification a fragment VP1 gene, DNA was 45 (66%) Navaho, 14 (56%) Flathead, 20 (69%) Chamorro. Genotyping viral DNAs these cohorts cycle sequencing...
<h3>Background</h3> Anti vascular endothelial growth factor (VEGF) therapy is an established treatment for various retinal diseases. Long-term data on injection frequencies and visual acuity (VA), however, are still rare. <h3>Methods</h3> Five-year analysis of real-life VA developments patterns from 2072 patients (2577 eyes; 33 187 injections) with chronically active disease undergoing pro-re-nata age-related macular degeneration (AMD), diabetic oedema (DME), vein occlusion (RVO) myopic...
Age-related macular degeneration (AMD) is the most common reason of visual impairment in elderly Western countries. The retinal pigment epithelial cells (RPE) causes secondarily adverse effects on neural retina leading to loss. aging characteristics RPE involve lysosomal accumulation lipofuscin and extracellular protein aggregates called "drusen". Molecular mechanisms behind aggregations are weakly understood. There intriguing evidence suggesting that SQSTM1/p62, together with autophagy, has...
SARS-CoV-2 is assumed to use angiotensin-converting enzyme 2 (ACE2) and other auxiliary proteins for cell entry. Recent studies have described conjunctival congestion in 0.8% of patients with laboratory-confirmed severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), there has been speculation that can be transmitted through the conjunctiva. However, it currently unclear whether epithelial cells express ACE2 its cofactors. In this study, a total 38 samples from patients, including 12...
Real-life clinical outcomes of patients treated with anti-VEGF drugs for neovascular age-related macular degeneration (nAMD), diabetic edema (DME), or secondary to branch retinal vein occlusion (BRVO) are often inferior results from randomized trials. This observational cohort study investigates treatment adherence and real-life within the first year treatment.A total 708 treatment-naïve (466 nAMD, 134 DME, 108 BRVO) were included. Patients followed a PRN protocol three intravitreal...
PurposeTo compare the efficacy and safety of abicipar every 8 weeks quarterly (after initial doses) versus ranibizumab 4 in treatment-naïve patients with neovascular age-related macular degeneration (AMD).DesignTwo randomized, multicenter, double-masked, parallel-group, active-controlled, phase 3 clinical trials (CEDAR, SEQUOIA) identical protocols were conducted. Data from both pooled for analysis.ParticipantsPatients active choroidal neovascularization secondary to AMD best-corrected...
The applications of deep sequencing technologies in life science research and clinical diagnostics have increased rapidly over the last decade. Although fast algorithms for data processing exist, intuitive, portable solutions analysis are still rare. For this purpose, we developed a web-based transcriptome database, which provides platform-independent, intuitive solution to easily explore compare ocular gene expression 100 diseased healthy human tissue samples from 15 different types...
Meta-analyses of case series non-arteritic central retinal artery occlusion (CRAO) indicate beneficial effects intravenous thrombolysis when initiated early after symptom onset. Randomized data are lacking to address this question.
Abstract Myeloid cells such as resident retinal microglia (MG) or infiltrating blood‐derived macrophages (Mϕ) accumulate in areas of ischemia and neovascularization (RNV) modulate neovascular eye disease. Their temporospatial distribution biological function this process, however, remain unclarified. Using state‐of‐the‐art methods, including cell‐specific reporter mice high‐throughput RNA sequencing (RNA Seq), study determined the extent MG proliferation Mϕ infiltration with RNV Cx3cr1...
Distinctive genotypes of JC virus have been described for the major continental landmasses. Studies on European-Americans and small cohorts in Europe showed predominantly Type 1. Types 2 7 are found Asia, 3 6 Africa. These differ sequence by about 1–3%. Each genotype may several subtypes which from each other 0·5–1%. The can be defined a distinctive pattern nucleotides typing region VP 1 gene. This genotyping approach has confirmed phylogenetic reconstruction using entire genome exclusive...
Infection of injury results in several systemic and central reactions termed the acute phase response (APR). Substantial evidence suggests that cytokines induced by microbes initiate APR. We compared APR rabbits a model bacterial stimulus, lipopolysaccharide (LPS), to viral polyriboinosinic:polyribocytidylic acid (poly I:C). The cytokine mRNA responses mouse macrophage cell line (RAW 264.7) LPS or poly I:C were also determined. Rabbits injected intravenously intracerebroventricularly with...
A number of key ocular diseases, including diabetic retinopathy and age-related macular degeneration, are characterized by localized areas epithelial or endothelial damage, which can ultimately result in the growth fragile new blood vessels, vitreous hemorrhage, retinal detachment. VEGF-A(165), principal neovascular agent angiogenic conditions, is formed proximal splice site selection its terminal exon 8. Alternative splicing this results an antiangiogenic isoform, VEGF-A(165)b,...
The next-generation ophthalmic anti-VEGF therapeutics must aim at being superior to the currently available agents with regard potency and improved drug delivery, while still stable safe use elevated concentrations. We show here generation of a set highly potent VEGF-A antagonistic DARPins (designed ankyrin repeat proteins) delivering these properties. single-digit picomolar affinity human were generated using ribosome display selections. Specific binding was confirmed by ELISA endothelial...
Retinal neovascularization has been intensively investigated in the mouse model of oxygen-induced retinopathy (OIR). Here, we studied contribution microglial cells to vascular regression during hyperoxic phase and retinal hypoxic phase.Mice expressing green fluorescent protein (GFP) under Cx3cr1 promoter labeling were kept 75% oxygen from postnatal day 7 (P7) P12. Microglial cell density was quantified at different time points positions flat mounts. activation determined by switch ramified...