A5066055190- Proteoglycans and glycosaminoglycans research
- Radiomics and Machine Learning in Medical Imaging
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- Heat shock proteins research
- Glycosylation and Glycoproteins Research
- Gene expression and cancer classification
- Breast Cancer Treatment Studies
- Breast Lesions and Carcinomas
- thermodynamics and calorimetric analyses
- Protease and Inhibitor Mechanisms
- Polyamine Metabolism and Applications
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Osteoarthritis Treatment and Mechanisms
- HER2/EGFR in Cancer Research
- Polysaccharides and Plant Cell Walls
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Growth Hormone and Insulin-like Growth Factors
- Cell Adhesion Molecules Research
- MRI in cancer diagnosis
- Wnt/β-catenin signaling in development and cancer
- Endoplasmic Reticulum Stress and Disease
University of Eastern Finland
2015-2024
Kuopio University Hospital
2014-2024
Finland University
2011-2019
Institute of Clinical Research
2015-2018
Clinical Research Management
2018
Vaasa Central Hospital
2010
Spanish National Cancer Research Centre
2010
Cancer Clinic
2010
Cancer Genetics (United States)
2010
National Cancer Institute
2010
Hyaluronan is an abundant and rapidly turned over matrix molecule between the vital cell layers of epidermis. In this study, epidermal growth factor (EGF) induced a coat hyaluronan 3–5-fold increase in its rate synthesis rat keratinocyte line that has retained ability for differentiation. EGF also increased perinuclear vesicles, suggesting concurrent enhancement endocytosis. Cell-associated was most elongated cells were stimulated to migrate by EGF, as determined vitroin wound healing assay....
Age-related macular degeneration (AMD) is the most common reason of visual impairment in elderly Western countries. The retinal pigment epithelial cells (RPE) causes secondarily adverse effects on neural retina leading to loss. aging characteristics RPE involve lysosomal accumulation lipofuscin and extracellular protein aggregates called "drusen". Molecular mechanisms behind aggregations are weakly understood. There intriguing evidence suggesting that SQSTM1/p62, together with autophagy, has...
Epitheliomesenchymal transition (EMT) is the process where cancer cells attain fibroblastic features and are thus able to invade neighboring tissues. Transcriptional factors zeb1, snai1 twist regulate EMT. We used immunohistochemistry investigate expression of in tumor stromal compartments by a large set breast carcinomas. The results were compared with estrogen progesterone receptor status, HER2 amplification, grade, histology, TNM status survival patients. Nuclear for was seen epithelial...
Previous in vitro studies have suggested interactions between hyaluronan (HA), CD44 and HER2. We studied the expression of HA a material 278 breast cancer cases, half which were HER2-positive. Intense stromal staining was associated with HER2 positivity, large tumor size, lymph node hormone receptor negativity, poor differentiation, high body mass index, increased relapse rate shortened overall survival. Among 139 HER2-positive intensity as most relapses occurred cases intense staining. The...
Purpose To compare the diagnostic accuracy of ultrasonographically (US)-guided fine-needle aspiration biopsy (FNAB) and core-needle (CNB) axillary lymph nodes (LNs) patients with newly diagnosed invasive breast cancer. Materials Methods This prospective single-center study had institutional review board approval, written informed consent was obtained. Between April 2011 March 2012, 178 consecutive (182 axillae) were evaluated by using US. Sixty-six axillae fulfilled inclusion criteria...
In response to various stress stimuli, heat shock genes are induced express proteins (Hsps). Previous studies have revealed that expression of is regulated both at transcriptional and posttranscriptional level, the rapid induction involves activation specific transcription factor, factor 1 (HSF1). Furthermore, can vary in intensity kinetics a signal- cell-type-dependent manner. this study, we demonstrate mechanical loading form hydrostatic pressure increases gene human chondrocyte-like...
Apparent diffusion coefficient (ADC) values are increasingly reported in breast MRI. As there is no standardized method for ADC measurements, we evaluated the effect of size region interest (ROI) to diagnostic utility and correlation prognostic markers cancer.This prospective study was approved by Institutional Ethics Board; need written informed consent retrospective analyses MRIs waived Chair Hospital District. We compared accuracy measurements from whole-lesion ROIs (WL-ROIs) small...
The role of mast cells in cutaneous melanoma remains unclear. Tryptase and chymase are serine proteinases major proteins cell secretory granules. Therefore, this study aimed to investigate the presence tryptase+ chymase+ benign malignant melanocytic lesions lymph node metastases melanomas. positively stained was correlated with clinicopathological characteristics invasive Paraffin-embedded sections 28 (13 intradermal, 10 compound, five junctional nevi) 26 dysplastic nevi, 15 in-situ...
The role of tumor-associated macrophages (TAMs) in cutaneous melanoma is controversial. TAMs include immunogenic and immunosuppressive subtypes, have distinct functions according to their microanatomical localization. Our aim was investigate benign, premalignant, malignant melanocytic lesions determine possible associations with tumor progression clinicopathological characteristics. In total, 184 tissue samples, including benign dysplastic nevi, in-situ melanomas, superficial (Breslow’s...
E-cadherin (CDH1) is a putative tumor suppressor gene implicated in breast carcinogenesis. Yet, whether risk factors or survival differ by expression unclear. We evaluated immunohistochemistry using tissue microarrays of 5,933 female invasive cancers from 12 studies the Breast Cancer Consortium. H-scores were calculated and case-case odds ratios (OR) 95% confidence intervals (CIs) estimated logistic regression. Survival analyses performed Cox regression models. All stratified estrogen...
Heat shock factors (HSFs) are the main transcriptional regulators of evolutionarily conserved heat response. Beyond cell stress, several studies have demonstrated that HSFs also contribute to a vast variety human pathologies, ranging from metabolic diseases cancer and neurodegeneration. Despite their evident role in mitigating cellular perturbations, functions HSF1 HSF2 physiological proteostasis remained inconclusive. Here, we analyzed comprehensive selection paraffin-embedded tissue...
Hyaluronan accumulation correlates with the degree of malignancy in many solid tumor types, including malignant endometrial carcinomas. To elucidate mechanism hyaluronan accumulation, we examined expression levels synthases (HAS1, HAS2 and HAS3) hyaluronidases (HYAL1 HYAL2), correlated them content HAS1-3 immunoreactivity.A total 35 tissue biopsies from patients, proliferative secretory endometrium (n = 10), post-menopausal 5), complex atypical hyperplasia 4), grade 1 8) 2 + 3 endometrioid...
Abstract Background Hyaluronan, a tumor promoting extracellular matrix polysaccharide, is elevated in malignant epithelial ovarian tumors, and associates with an unfavorable prognosis. To explore possible contributors to the accumulation of hyaluronan, we examined expression hyaluronan synthases ( HAS1 , HAS2 HAS3 ) hyaluronidases HYAL1 HYAL2 ), correlated hyaluronidase enzyme activity content HAS1–3 immunoreactivity. Methods Normal ovaries (n = 5) 34 serous divided into 4 groups: grades 1+2...
Epithelial-mesenchymal transition (EMT) is a crucial process in tumorigenesis since tumor cells attain fibroblast-like features enabling them to invade surrounding tissue. Two transcription factors, TWIST and SNAI1, are fundamental regulating EMT. Immunohistochemistry was used study the expression of SNAI1 109 pharyngeal squamous cell carcinomas. Tumors with intense stromal staining relapsed more frequently (p = 0.04). both positive immunoreactivity stroma were at least Stage II 0.05)...
CD44 is a multifunctional adhesion molecule typically upregulated in malignant, inflamed and injured tissues. Due to its ability bind multiple ligands present the tumor microenvironment, it promotes cellular functions related tumorigenesis. Recent data has shown that principal ligand hyaluronan (HA) are carried by extracellular vesicles (EV) derived from stem cells, but role of EV shedding not been studied so far. To answer this question, we utilized CD44-negative human gastric carcinoma...
Abstract Treatment of prostate cancer confronts resistance to androgen receptor (AR)-targeted therapies. AR-associated coregulators and chromatin proteins hold a great potential for novel therapy targets. Here, we employed powerful chromatin-directed proteomics approach termed ChIP-SICAP uncover the composition protein network, chromatome, around endogenous AR in castration resistant (CRPC) cells. In addition several expected coregulators, chromatome contained many nuclear not previously...
Abstract Hyaluronan (HA) accumulation in clear cell renal carcinoma (ccRCC) is associated with poor prognosis; however, its biology and role tumorigenesis are unknown. RNA sequencing of 48 HA-positive HA-negative formalin-fixed paraffin-embedded (FFPE) samples was performed to identify differentially expressed genes (DEG). The DEGs were subjected pathway gene enrichment analyses. Cancer Genome Atlas Kidney Renal Clear Cell Carcinoma (TCGA-KIRC) data used for the cluster analysis. In total,...
Hyaluronan is a major component of the epidermal extracellular matrix, actively synthesized by keratinocytes and shows fast matrix turnover in stratified epithelium. We probed importance hyaluronan synthesis establishing cell lines carrying exogenous synthase 2 (Has2) gene sense antisense orientations to increase decrease their synthesis, respectively. Compared with transfected vector only, most clones containing Has2 migrated faster an vitro wounding assay, whereas cells more slowly. showed...