A5088605616- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Osteoarthritis Treatment and Mechanisms
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
- Tendon Structure and Treatment
- Connective tissue disorders research
- Polysaccharides and Plant Cell Walls
- Knee injuries and reconstruction techniques
- Carbohydrate Chemistry and Synthesis
- Veterinary Orthopedics and Neurology
- Skin Protection and Aging
- Silk-based biomaterials and applications
- HER2/EGFR in Cancer Research
- Lower Extremity Biomechanics and Pathologies
- Spine and Intervertebral Disc Pathology
- Platelet Disorders and Treatments
- Wound Healing and Treatments
- Protease and Inhibitor Mechanisms
- Veterinary Equine Medical Research
- Ocular Surface and Contact Lens
- Advancements in Transdermal Drug Delivery
- Exercise and Physiological Responses
- Adipose Tissue and Metabolism
University of Eastern Finland
2012-2021
Finland University
2011-2017
Institute for Biomedicine
2016
Kuopio University Hospital
1992-2012
University of Debrecen
2012
Uzsoki Hospital
2012
Oulu University Hospital
2008
University of Turku
1977-2006
Cleveland Clinic
1998-2001
Cleveland Clinic Lerner College of Medicine
2001
Aims High amounts of tumour‐associated macrophages ( TAM s) and hyaluronan HA ) correlate with tumour aggressiveness in breast cancer, but the relationship between these parameters is unclear. The aim this study was to assay numbers s 278 human cancer cases, their correlations ‐related factors, clinical variables, outcome. Methods results immunoreactivities for CD 163 68 were considered as indicators M2‐like all s, respectively. counted at least four hot spots, averaged represent each...
CD44 is the primary cell surface receptor for extracellular matrix glycosaminoglycan hyaluronan. Here we determined relative avidities of unlabeled hyaluronan preparations by their ability to block binding fluorescein-conjugated a variety cells. We show that at complex interplay multivalent events affected size ligand, quantity and density CD44, activation state as cell-specific factors and/or treatment with CD44-specific monoclonal antibody (mAb). Using low <i>M</i> <sub>r</sub> oligomers...
Lymphocyte interactions with high endothelial venules (HEV) are important to the in vivo migration of normal and neoplastic lymphocyte populations. We have previously described an 85- 95-kDa surface glycoprotein(s) defined by mAb Hermes-1, that is involved recognition HEV human lymphocytes: antibodies against distinct epitopes Hermes-1 Ag differentially inhibit binding lymph node, mucosal, or synovial HEV. Here we characterize further Hermes-1-defined glycoproteins. No well differences were...
Hyaluronan synthesized in the epidermis has an exceptionally short half-life, indicative of its catabolism by epidermal keratinocytes. An intracellular pool endogenously hyaluronan, from 1 to 20 fg/cell, inversely related cell density, was observed cultured rat More than 80% hyaluronan small (<90 kDa). Approximately 25% newly endocytosed keratinocytes and had a half-life 2–3 h. A biotinylated aggrecan G<sub>1</sub> domain/link protein probe demonstrated vesicles ∼100 nm diameter close plasma...
Abstract Cultured bovine articular cartilage was subjected to 50 ms, 0.5–1.0 MPa compressions repeated at intervals of 2–60 s for 1.5 h and simultaneously labeled with 35 SO 4 . The compression delivered a 4‐mm‐diameter nonporous loading head on an 8‐mm‐diameter explant. This method created directly compressed (central) uncompressed (border) areas within the tissue. Analysis whole explant under 0.5 load showed significantly increased incorporation by 2‐ 4‐s but not 20‐ 60‐s intervals. When...
Abstract Casting of the right knee (stifle) joints young beagle dogs for 11 weeks caused up to 48% reduction in glycosaminoglycan (GAG) concentration uncalcified articular cartilage, as assessed by a new microspectrophotometric method. The GAGs were depleted mainly superficial zone cartilage. Although thickness cartilage was not decreased, calcified under tidemark thinned 6–25% at femoral condyles. increased weight‐bearing limb opposite one splint be augmented 19% and GAG 25–35%...
It has been shown that extracellular glycosaminoglycans (GAGs) limit the gene transfer by cationic lipids and polymers. The purpose of this study was to clarify how interactions with anionic GAGs (hyaluronic acid heparan sulfate) modify cellular uptake distribution lipoplexes polyplexes. Experiments on DNA GFP reporter expression showed decreased can rarely be explained lower uptake. In most cases, is not changed GAG binding or Reporter blocked sulfate, but it increased hyaluronic acid;...
Hyaluronan and chondroitin/dermatan sulfate are glycosaminoglycans that play major roles in the biomechanical properties of a wide variety tissues, including cartilage. A chain can be divided into three regions: (1) single linkage region oligosaccharide, through which is attached to its proteoglycan core protein, (2) numerous internal repeat disaccharides, comprise bulk chain, (3) nonreducing terminal saccharide structure. Each these regions has own level microheterogeneity structure, varies...