A5068559729- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Protein Tyrosine Phosphatases
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Signaling Pathways in Disease
- Immune cells in cancer
- Collagen: Extraction and Characterization
- Fibroblast Growth Factor Research
- Estrogen and related hormone effects
- Peptidase Inhibition and Analysis
- Erythrocyte Function and Pathophysiology
- Cancer Immunotherapy and Biomarkers
- Critical Theory and Philosophy
- Antimicrobial Peptides and Activities
- Cytokine Signaling Pathways and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal Respiratory Health Research
- Antifungal resistance and susceptibility
University of British Columbia
2015-2025
James Graham Brown Foundation
2024
University of Central Florida
2021
Beihang University
2021
Child and Family Research Institute
2007-2010
BC Cancer Agency
2010
Wake Forest University
2010
Heinrich Heine University Düsseldorf
2010
Center of Advanced European Studies and Research
2010
University of the West Indies
2006
Abstract Galectins are a family of mammalian β-galactoside-binding proteins that positively and negatively regulate T cell death. Extracellular galectin-1 directly induces death cells thymocytes, while intracellular galectin-3 blocks In contrast to the antiapoptotic function galectin-3, we demonstrate extracellular human thymocytes cells. However, events in galectin-3- galectin-1-induced differ number ways. Thymocyte subsets different susceptibility two galectins: whereas kills...
CD45 is a family of high molecular weight leukocyte cell surface glycoproteins. Recently, two related subregions the cytoplasmic domain have been shown to 30-40% amino acid identity with human placental protein phosphotyrosine phosphatase, and isolated from spleen was found exhibit intrinsic phosphatase (EC 3.1.3.48) activity. In present studies, we demonstrate that each known isoforms murine has an equivalent basal level activity establish this enzymatic associated glycoprotein. Studies...
Leukocytes express a family of plasma membrane proteins called CD45 or the leukocyte common antigen. Isoforms various molecular masses, 180-240 kDa, are produced by alternative splicing and usage three exons, named A, B, C, that encode N-terminal portion external domain. By using monoclonal antibodies precipitate B exon-dependent exon-independent isoforms we find both murine CD4+ CD8+ T cells selectively down-regulate forms during an immune response. This change was monitored...
Abstract Lymphocyte CD44 interactions with hyaluronan localized on the endothelium have been demonstrated to mediate rolling and regulate lymphocyte entry into sites of chronic inflammation. Because neutrophils also express CD44, we investigated role in multistep process neutrophil recruitment. CD44−/− wild-type control mice were intrascrotally injected neutrophil-activating chemokine, MIP-2, leukocyte kinetics cremasteric microcirculation 4 h subsequently using intravital microscopy....
Abstract Y-box binding protein-1 (YB-1) is an oncogenic transcription/translation factor expressed in >40% of breast cancers, where it associated with poor prognosis, disease recurrence, and drug resistance. We questioned whether this may be linked to the ability YB-1 induce expression genes cancer stem cells such as CD44 CD49f. Herein, we report that binds CD49f promoters transcriptionally upregulate their expressions. The introduction wild-type (WT) or activated P-YB-1S102...
The human cathelicidin peptide, LL-37, is a host defense peptide with wide range of immunomodulatory activities and modest direct antimicrobial properties. LL-37 can exert both pro- anti-inflammatory effects modulate the proinflammatory responses peripheral blood monocytes epithelial cells. In this study, we evaluated effect on mouse bone marrow-derived macrophages (BMDM) tissue in vitro vivo. dramatically reduced TNF-α NO levels produced by LPS IFN-γ-polarized M1-BMDM slightly reactive...
Regulation of cell adhesion is important for immune system function. CD44 a tightly regulated molecule present on leukocytes and implicated in their attachment to endothelium during an inflammatory response. The proinflammatory cytokine tumor necrosis factor–α, but not interferon-γ, was found convert from its inactive, nonbinding form active by inducing the sulfation CD44. This posttranslational modification required CD44-mediated binding extracellular matrix component hyaluronan vascular...
We have studied the erythrocytes of two individuals (P. L. and K. W.) who lack Gerbich (Ge) blood-group antigen. The P. W. were not reactive with monoclonal antibodies (NBTS/BRIC 4 NBTS/BRIC 10) which reacted normal erythrocytes. membranes appeared to three minor sialoglycoproteins (beta, beta 1 gamma). These found be associated cytoskeletons Approx. 10% frankly elliptocytic. suggest that one or more may play a part in maintaining discoid shape human erythrocyte.
Latent HIV-1 (human immunodeficiency virus-1) provirus is unaffected by current AIDS (acquired syndrome) therapies. We show here that chaetocin, an SUV39H1 histone methyltransferase inhibitor, causes 25-fold induction of latent expression, while producing minimal toxicity and without causing T cell activation. Induction associated with loss H3 lysine 9 (H3K9) trimethylation at the long terminal repeat (LTR) promoter, a corresponding increase in H3K9 acetylation. The effect chaetocin...
Summary Gut microbial community properties of mammals are thought to be partly shaped by a combination host immunity and environmental factors, but their relative importance is not firmly established. To address this gap, we first characterized the faecal bacteria mice with functioning immune system (wild‐type, WT ), defective responses ( CD 45), lacking an adaptive RAG both dysfunctions (45 ). Using fingerprinting 16 S rRNA genes, observed significant differences in gut microbiota...
Abstract The extracellular matrix glycosaminoglycan, hyaluronan, has been described as a regulator of tissue inflammation, with hyaluronan fragments reported to stimulate innate immune cells. High molecular mass is normally present in tissues, but upon inflammation lower are generated. It unclear if these induce an inflammatory response or consequence inflammation. In this study, mouse bone marrow derived macrophages and dendritic cells (DCs) were stimulated various sizes from different...
CD20, a non-glycosylated cell-surface protein expressed exclusively on B lymphocytes, is one of family 4-pass transmembrane molecules that also includes the β chain high affinity receptor for IgE. The precise function CD20 unknown, although in vitro effects CD20-specific antibodies resting cells indicate it able to transduce an extracellular signal affecting G0/G1 cell cycle transition. Previous studies have demonstrated CD20-initiated intracellular signals involve tyrosine kinase activation...
ABSTRACT Cryptococcus neoformans, an invasive basidiomycete fungal pathogen, causes one of the most prevalent, life-threatening diseases in immunocompromised individuals and accounts for ~19% AIDS-associated deaths. Therefore, understanding pathogenesis C. neoformans its interactions with host immune system is critical developing therapeutics against cryptococcosis. Previous studies demonstrated that cells lacking polyphosphate (polyP), immunomodulatory polyanionic storage molecule, display...
The T-cell receptor (TCR) zeta subunit is an important component of the TCR complex, involved in signal transduction events following engagement. In this study, we showed that chain constitutively tyrosine phosphorylated to similar extents thymocytes and lymph node T cells. Approximately 35% tyrosine-phosphorylated (phospho zeta) precipitated from total cell lysates appeared be surface associated. Furthermore, constitutive phosphorylation cells occurred independently antigen stimulation did...
CD45 is a transmembrane two-domain tyrosine phosphatase required for efficient signal transduction initiated by lymphocyte antigen receptors. As with most phosphatases, the role of second domain unclear. In this study, recombinant cytoplasmic proteins purified from bacteria were used to evaluate function individual domains. A protein expressing membrane-proximal region, first domain, and spacer region (rD1) was catalytically active found exist primarily as dimer. contrast this, carboxy tail...