A5055669866- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Retinoids in leukemia and cellular processes
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Antiplatelet Therapy and Cardiovascular Diseases
- Platelet Disorders and Treatments
- Nitric Oxide and Endothelin Effects
- Sphingolipid Metabolism and Signaling
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Peroxisome Proliferator-Activated Receptors
- Cell Adhesion Molecules Research
- Anesthesia and Sedative Agents
- Pain Mechanisms and Treatments
- Circadian rhythm and melatonin
- Religion, Ecology, and Ethics
- Animal Nutrition and Physiology
- Animal Genetics and Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Human-Animal Interaction Studies
- Receptor Mechanisms and Signaling
- Erythrocyte Function and Pathophysiology
- Environmental Philosophy and Ethics
Boeing (Australia)
2024
University of East Anglia
2022
University of New Mexico
2012-2020
Babraham Institute
2018-2019
Cardiff University
2009-2017
Zoetis (United States)
2015
Institute of Infection and Immunity
2010-2013
Tenovus Cancer Care
2009
University of Wales
2002-2007
University of Calgary
2006-2007
To study the mechanisms involved in leukocyte recruitment induced by local bacterial infection within CNS, we used intravital microscopy to visualize interaction between leukocytes and microvasculature brain. First, showed that intracerebroventricular injection of LPS could cause significant rolling adhesion brain postcapillary venules wild-type mice, while negligible was observed TLR4-deficient C57BL/10ScCr mice CD14 knockout suggesting is mediated TLR4/CD14-bearing cells. Moreover, reduced...
Nitration of unsaturated fatty acids such as linoleate by NO-derived reactive species forms novel derivatives (including nitrolinoleate [LNO 2 ]) that can stimulate smooth muscle relaxation and block platelet activation either NO/cGMP or cAMP-dependent mechanisms. Here, LNO was observed to inhibit human neutrophil function. , but not linoleic acid the nitrated amino 3-nitrotyrosine, dose-dependently (0.2 1 μmol/L) inhibited superoxide (O ·− ) generation, Ca 2+ influx, elastase release, CD11b...
Aminophospholipid (APL) trafficking across the plasma membrane is a key event in cell activation, apoptosis, and aging required for clearance of dying cells coagulation. Currently phospholipid molecular species externalized are unknown. Using lipidomic method, we show that thrombin, collagen, or ionophore-activated human platelets externalize two phosphatidylserines (PSs) five phosphatidylethanolamines (PEs). Four percent total cellular PE/PS pool (∼300 ng/2 × 10 8 cells, thrombin), via...
In this study, murine peritoneal macrophages from naïve lavage were found to generate four phospholipids that contain 12-hydroxyeicosatetraenoic acid (12-HETE). They comprise three plasmalogen and one diacyl phosphatidylethanolamines (PEs) (16:0p, 18:1p, 18:0p, 18:0a at sn-1) are absent in 12/15-lipoxygenase (12/15-LOX)-deficient mice. generated acutely response calcium mobilization, primarily cell-associated, detected on the outside of plasma membrane. Levels 12-HETE-PEs a similar range...
Intestinal health is important for maximizing the health, welfare, and performance of poultry. Traditionally, due to lack available technologies, our knowledge diversity microbial communities within chicken intestine had been oversimplified. Recent progress in technology suitable community analysis has evolved understanding. New molecular technologies allow a detailed physiological assessment, including quantification individual species their metabolites. One area research assessed shifts...
Oxidized phospholipids (oxPLs) generated nonenzymatically display pleiotropic biological actions in inflammation. Their generation by cellular cyclooxygenases (COXs) is currently unknown. To determine whether platelets generate prostaglandin (PG)-containing oxPLs, then characterize their structures and mechanisms of formation, we applied precursor scanning-tandem mass spectrometry to lipid extracts agonist-activated human platelets. Thrombin, collagen, or ionophore activation stimulated...
<h3>Background</h3> Seasonal influenza A infection affects a significant cohort of the global population annually, resulting in considerable morbidity and mortality. Therapeutic strategies are limited efficacy, during pandemic outbreak would only be available to minority population. Over-the-counter medicines routinely taken by individuals suffering from influenza, but few studies have been conducted determine their effectiveness reducing pulmonary immunopathology or influence they exert...
To investigate the role of death receptor 3 (DR-3) and its ligand tumor necrosis factor-like molecule 1A (TL1A) in early stages inflammatory arthritis.Antigen-induced arthritis (AIA) was generated C57BL/6 mice deficient DR-3 gene (DR3(-/-) ) their DR3(+/+) (wild-type) littermates by priming intraarticular injection methylated bovine serum albumin. The joints were sectioned analyzed histochemically for damage to cartilage expression DR3, TL1A, Ly-6G (a marker neutrophils), gelatinase matrix...
Intestinal health is important for maximising the health, welfare, and performance of poultry. In addition, intestinal issues in poultry can have devastating financial impacts producers, food safety concerns consumers. Until recently, were seen as a handful known infectious agents leading to set severe identifiable named diseases. There however an emerging area which depicts more complex multifaceted system than previously known. Recent progress technology suitable microbial community...
Airway microvascular leak (MVL) involves the extravasation of proteins from post-capillary venules into surrounding tissue. MVL is a cardinal sign inflammation and an important feature airway inflammatory diseases such as asthma. PGE2, product COX-mediated metabolism arachidonic acid, binds to four receptors, termed EP1–4. PGE2 has wide variety effects within airway, including modulation inflammation, sensory nerve activation tone. However, effect on receptor/s that mediate this have not...
Abstract The detailed mechanisms by which acutely activated leukocytes metabolize NO and regulate its bioactivity are unknown. Therefore, healthy, chronic granulomatous disease (CGD) or myeloperoxidase (MPO)-deficient human neutrophils were examined for their ability to consume attenuate signaling. fMLP PMA activation of healthy caused consumption that was fully blocked NADPH oxidase inhibition, absent in CGD neutrophils. Studies using MPO-deficient neutrophils, enzyme inhibitors,...
Activated platelets generate an eicosanoid proposed to be 8-hydroxy-9,10-dioxolane A3 (DXA3). Herein, we demonstrate that significant amounts of DXA3 are rapidly attached phosphatidylethanolamine (PE) forming four esterified eicosanoids, 16:0p, 18:0p, 18:1p and 18:0a/DXA3-PEs can activate neutrophil integrin expression. These lipids comprise the majority generated by platelets, formed in ng (24.3±6.1 ng/2×108) remain membrane bound. Pharmacological studies revealed DXA3-PE formation involves...
We examine the influence of quantum dot (QD) morphology on optical properties two-dimensional (2D) GaSb/GaAs multilayers, with and without three-dimensional nanostructures. Using nanostructure sizes from scanning transmission electron microscopy local Sb compositions local-electrode atom-probe tomography as input into self-consistent Schrödinger–Poisson simulations based 8 × k·p theory, we compute confinement energies for QDs, circular arrangements smaller termed QD-rings, 2D layers GaAs...
PGHS-2 (prostaglandin H synthase-2) is induced in mammalian cells by pro-inflammatory cytokines tandem with iNOS [high-output (‘inducible’) nitric oxide synthase], and co-localized nitrotyrosine human atheroma macrophages. Herein, murine J774.2 macrophages incubated lipopolysaccharide interferon γ showed induction of generated NO using that could be completely depleted 12(S)-HPETE [12(S)-hydroperoxyeicosatetraenoic acid; 2.4 μM] or hydrogen peroxide (500 μM) (0.42±0.084 0.38±0.02...
Data for flight loads testing is traditionally gathered using strain gages which are calibrated known load profiles in a laboratory setting. Accurate calibration of complex indeterminate structures have historically been challenging because it difficult to determine prior loading structural locations will yield gage response correlates well with external loads. A novel method presented leverages modern FEA capabilities, conjunction machine learning techniques, review trends over large...