A5037222936- Eicosanoids and Hypertension Pharmacology
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Inflammatory mediators and NSAID effects
- Asthma and respiratory diseases
- Fatty Acid Research and Health
- Peroxisome Proliferator-Activated Receptors
- Analytical Chemistry and Chromatography
- Lipid metabolism and biosynthesis
- Alcohol Consumption and Health Effects
- Sphingolipid Metabolism and Signaling
- Antioxidant Activity and Oxidative Stress
- Cholesterol and Lipid Metabolism
- Pharmacogenetics and Drug Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Free Radicals and Antioxidants
- Metabolism and Genetic Disorders
- Advanced Chemical Sensor Technologies
- Lipid Membrane Structure and Behavior
- Cinema and Media Studies
- Pharmacological Effects and Assays
- Allergic Rhinitis and Sensitization
- Neonatal Respiratory Health Research
- Adipose Tissue and Metabolism
Midwestern State University
2024
Midwestern University
2021-2024
United States Military Academy
2024
University of Colorado Denver
2014-2023
Weatherford (Norway)
2010-2023
University of Colorado Anschutz Medical Campus
2013-2022
Children's Healthcare of Atlanta
2022
Western University of Health Sciences
2022
University Hospital Limerick
2022
Northwestern University
2021
The focus of the present study was to define human plasma lipidome and establish novel analytical methodologies quantify large spectrum lipids. Partial lipid analysis is now a regular part every patient's blood test physicians readily regularly prescribe drugs that alter levels major lipids such as cholesterol triglycerides. Plasma contains many thousands distinct molecular species fall into six main categories including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids,...
The LIPID MAPS Structure Database (LMSD) is a relational database encompassing structures and annotations of biologically relevant lipids. Structures lipids in the come from four sources: (i) Consortium's core laboratories partners; (ii) identified by experiments; (iii) computationally generated for appropriate lipid classes; (iv) manually curated BANK, LIPIDAT other public sources. All LMSD are drawn consistent fashion. In addition to classification-based retrieval lipids, users can search...
Murine mastocytoma cells treated with calcium ionophore A23187 produced a slow-reacting substance (SRS) that caused guinea pig ileum to contract. The response was reversed by the SRS antagonist FPL 55712. On basis of isotope incorporation experiments, spectroscopy, and chemical degradations, identified as cysteine-containing derivative 5-hydroxy-7,9,11,14-icosatetraenoic acid. This amino acid attached in thioether linkage at C-6. is structurally related previously epoxy dihydroxy metabolites...
A comprehensive and standardized system to report lipid structures analyzed by MS is essential for the communication storage of lipidomics data. Herein, an update on both LIPID MAPS classification shorthand notation presented categories Fatty Acyls (FA), Glycerolipids (GL), Glycerophospholipids (GP), Sphingolipids (SP), Sterols (ST). With its major changes, i.e., annotation ring double bond equivalents number oxygens, updated facilitates reporting newly delineated oxygenated species as well....
Common organic matrix-assisted laser desorption/ionization (MALDI) matrices, 2,5-dihydroxybenzoic acid, 3,5-dimethoxy-4-hydroxycinnamic and α-cyano-4-hydroxycinnamic were found to undergo sublimation without decomposition under conditions of reduced pressure elevated temperature. This solid vapor-phase transition was exploited apply MALDI matrix onto tissue samples over a broad surface in solvent-free application for mass spectrometric imaging. Sublimation produced an even layer small...
Abstract Lipids are produced, transported, and recognized by the concerted actions of numerous enzymes, binding proteins, receptors. A comprehensive analysis lipid molecules, “lipidomics,” in context genomics proteomics is crucial to understanding cellular physiology pathology; consequently, biology has become a major research target postgenomic revolution systems biology. To facilitate international communication about lipids, classification lipids with common platform that compatible...
At a time when more and of what people learn both in formal courses everyday life is mediated by technology, Learning Online provides much-needed guide to different forms applications online learning. This book describes how learning being used K-12 higher education settings as well outside school. Particular technologies, such MOOCs (massive open courses), multi-player games, analytics, adaptive practice environments, are described terms design principles, implementation, contexts use....
The spectrum of nonalcoholic fatty liver disease (NAFLD) includes steatosis, steatohepatitis (NASH), and cirrhosis. Recognition timely diagnosis these different stages, particularly NASH, is important for both potential reversibility limitation complications. Liver biopsy remains the clinical standard definitive diagnosis. Diagnostic tools minimizing need invasive procedures or that add information to histologic data are in novel management strategies growing epidemic NAFLD. We describe an...
Recent developments in MALDI have enabled direct detection of lipids as intact molecular species present within cellular membranes. Abundant lipid-related ions are produced from the analysis thin tissue slices when sequential spectra acquired across a surface that has been coated with matrix. The lipid-derived can often be distinguished other biomolecules because significant mass defect these due to large number covalently bound hydrogen atoms hydrophobic molecules such lipids. Collisional...
Human platelets acutely increase mitochondrial energy generation following stimulation. Herein, a lipidomic circuit was uncovered whereby the substrates for this are exclusively provided by cPLA2, including multiple fatty acids and oxidized species that support via β-oxidation. This indicates acute lipid membrane remodeling is required to energetic demands during platelet activation. Phospholipase activity linked metabolism, revealing cPLA2 as central regulator of both lipidomics flux. Using...
Severe pulmonary hypertension (PH) occurs in a primary or “unexplained” form and group of secondary forms associated with number diseases. Because the lung tissue from patients severe PH demonstrates complex vascular lesions, which contain inflammatory cells, we wondered whether was “under oxidative stress.” We used immunohistochemistry to localize nitrotyrosine 8-hydroxy guanosine sections PH. In some extracts, eicosanoid metabolites 5-oxo-eicosatetraenoic acid, leukotriene B4...
Transformation of leukotriene (LT) A4 into C4 has been found to be carried out by human platelets in a rather efficient manner. LTC4 was characterized combination high performance liquid chromatography, UV spectrophotometry, use labeled precursor, guinea pig ileum bioassay, and enzyme immunoassay. LTA4 metabolism substrate-dependent, time-dependent, proportional platelet concentration even at sub- or supraphysiological levels (0.0019-1 X 10(9) platelets/ml). Neither plasma alone nor the...
Collision-induced dissociation (CID) of the [M + H]+ glycerophospholipids typically results in abundant fragment ions that are related to polar head group or loss group. An exception this general rule occurs for glycerophosphoethanolamines (GPEtn), which a class phospholipids can have an acyl, 1-O-alkyl, 1-O-alk-1'-enyl as substituent at sn-1 position. The CID diacyl-GPEtn expected phosphoethanolamine (141 Da). Therefore, constant neutral 141 Da has been used diagnostic tool determination...
Synthetic high affinity peroxisome proliferator-activated receptor (PPAR) agonists are known, but biologic ligands of low affinity. Oxidized density lipoprotein (oxLDL) is inflammatory and signals through PPARs. We showed, by phospholipase A1 digestion, that PPARγ in oxLDL arise from the small pool alkyl phosphatidylcholines LDL. identified an abundant oxidatively fragmented phospholipid oxLDL, hexadecyl azelaoyl phosphatidylcholine (azPC), as a ligand agonist for PPARγ. [3H]azPC bound...