A5042625397- Metabolomics and Mass Spectrometry Studies
- Sphingolipid Metabolism and Signaling
- Liver Disease Diagnosis and Treatment
- Cholesterol and Lipid Metabolism
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Diet and metabolism studies
- Gut microbiota and health
- Adipose Tissue and Metabolism
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Fatty Acid Research and Health
- Neonatal Respiratory Health Research
- Adipokines, Inflammation, and Metabolic Diseases
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and disorders
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Erythrocyte Function and Pathophysiology
- Osteoarthritis Treatment and Mechanisms
- Diabetes, Cardiovascular Risks, and Lipoproteins
University Hospital Regensburg
2016-2025
University of Regensburg
2016-2025
Washington Center
2018
University of Washington
2018
Helmholtz Zentrum München
2015
Hangzhou First People's Hospital
2013
Bioengineering Center
2013
National Center for Genetic Engineering and Biotechnology
2013
University Medical Center
2011
Columbia University
2000
Accurate profiling of lipidomes relies upon the quantitative and unbiased recovery lipid species from analyzed cells, fluids, or tissues is usually achieved by two-phase extraction with chloroform. We demonstrated that methyl-tert-butyl ether (MTBE) allows faster cleaner well suited for automated shotgun profiling. Because MTBE's low density, lipid-containing organic phase forms upper layer during separation, which simplifies its collection minimizes dripping losses. Nonextractable matrix a...
There is a need for standardized, practical annotation structures of lipid species derived from mass spectrometric approaches; i.e., high-throughput data obtained instruments operating in either high- or low-resolution modes. This proposal based on common, officially accepted terms and builds upon the LIPID MAPS terminology. It aims to add defined levels information below nomenclature, as detailed chemical structures, including stereochemistry, are usually not automatically provided by...
A comprehensive and standardized system to report lipid structures analyzed by MS is essential for the communication storage of lipidomics data. Herein, an update on both LIPID MAPS classification shorthand notation presented categories Fatty Acyls (FA), Glycerolipids (GL), Glycerophospholipids (GP), Sphingolipids (SP), Sterols (ST). With its major changes, i.e., annotation ring double bond equivalents number oxygens, updated facilitates reporting newly delineated oxygenated species as well....
Excessive uptake of atherogenic lipoproteins such as modified low-density lipoprotein complexes by vascular macrophages leads to foam cell formation, a critical step in atherogenesis. Cholesterol efflux mediated high-density (HDL) constitutes protective mechanism against macrophage lipid overloading. The molecular mechanisms underlying this reverse cholesterol transport process are currently not fully understood. To identify effector proteins that involved and release, we searched for genes...
The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, constituent atherogenic lipoproteins, binds and induces clustering co-receptors rafts. In resting cells, was associated with CD55, Fcγ-receptors CD32 CD64 pentaspan CD47. Ceramide further recruited complement 3 (CD11b/CD18) CD36 into proximity CD14. Lipopolysaccharide, addition, induced co-clustering Toll-like 4,...
Glycerophospholipid and sphingolipid species their bioactive metabolites are important regulators of lipoprotein cell function. The aim the study was to develop a method for lipid profiling separated classes. Human serum lipoproteins VLDL, LDL, HDL 21 healthy fasting blood donors were by fast performance liquid chromatography (FPLC) from 50 microl serum. Subsequently, phosphatidylcholine (PC), lysophosphatidylcholine, sphingomyelin (SM), ceramide (CER), phosphatidylethanolamine (PE),...
Human blood is a self-regenerating lipid-rich biological fluid that routinely collected in hospital settings. The inventory of lipid molecules found plasma (plasma lipidome) offers insights into individual metabolism and physiology health disease. Disturbances the lipidome also occur conditions are not directly linked to metabolism; therefore, lipidomics based on MS an emerging tool array clinical diagnostics disease management. However, challenges exist translation such lipidomic data...
Abstract Interactions between the gut microbial ecosystem and host lipid homeostasis are highly relevant to physiology metabolic diseases. We present a comprehensive multi-omics view of effect intestinal colonization on hepatic metabolism, integrating transcriptomic, proteomic, phosphoproteomic, lipidomic analyses liver plasma samples from germfree specific pathogen-free mice. Microbes induce monounsaturated fatty acid generation by stearoyl-CoA desaturase 1 polyunsaturated elongation...
As the gut microbiota contributes to metabolic health, it is important determine specific diet-microbiota interactions that influence host metabolism. Bile acids and dietary fat source can alter phenotypes of diet-induced obesity, but interplay with intestinal microorganisms unclear. Here, we investigated consequences diets enriched in primary bile or without addition lard palm oil, studied structure functions mice. In combination acids, fed male C57BL/6N mice for a period 8 weeks enhanced...
The state-of-art in the lipidomic analysis is summarized here to provide overview of available sample preparation strategies, mass spectrometry (MS)-based methods for qualitative lipids, and quantitative MS approaches high-throughput clinical workflows. Major challenges terms widely accepted best practices analysis, nomenclature, standards data reporting are discussed as well.
Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve survival time patients, who are often diagnosed too late at an incurable stage. Several studies report dysregulation lipid metabolism in tumor cells, suggesting that changes blood lipidome accompany growth. Here we show comprehensive mass spectrometric determination a wide range serum lipids reveals statistically significant differences between pancreatic patients and healthy controls, as...
Gut microbiota represents a therapeutic target for obesity. We hypothesize that B. uniformis CECT 7771 combined with wheat bran extract (WBE), its preferred carbon source, may exert superior anti-obesity effects. performed 17-week intervention in diet-induced obese mice receiving either uniformis, WBE, or their combination to identify interactions and independent actions on metabolism immunity. WBE was the most effective intervention, curbing weight gain adiposity, while exerting more modest...
Recent data indicate that ceramide (Cer) and lysophosphatidylcholine (LPC) regulate immune cell functions. Since these bioactive lipids are generated in blood plasma by inflammatory lipases, we hypothesized they may be involved the process of acute systemic sepsis. In order to provide support for this hypothesis, analyzed levels Cer LPC quantitative tandem mass spectrometry 102 sepsis patients starting with day at which criteria were fulfilled first time, as well on 4 11. The values compared...
Ceramide (CER) is an important signaling molecule involved in a variety of cellular processes, including differentiation, cell growth, and apoptosis. Currently, different techniques are applied for CER quantitation, some which relatively insensitive and/or time consuming. Tandem mass spectrometry with its high selectivity sensitivity very useful technique detection low abundant metabolites without prior purification or derivatization. In contrast to existing methods, the developed...