A5091124551- Animal testing and alternatives
- Immune Response and Inflammation
- Computational Drug Discovery Methods
- Effects and risks of endocrine disrupting chemicals
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Immunotoxicology and immune responses
- Metabolomics and Mass Spectrometry Studies
- Carcinogens and Genotoxicity Assessment
- Neuroscience and Neural Engineering
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Health Systems, Economic Evaluations, Quality of Life
- Meta-analysis and systematic reviews
- Axial and Atropisomeric Chirality Synthesis
- Anesthesia and Neurotoxicity Research
- Viral Infectious Diseases and Gene Expression in Insects
- Sepsis Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Antimicrobial Peptides and Activities
- Immune Cell Function and Interaction
- Chemistry and Chemical Engineering
- Health, Environment, Cognitive Aging
- Meat and Animal Product Quality
- Molecular spectroscopy and chirality
- Immune cells in cancer
University of Konstanz
2016-2025
Johns Hopkins University
2016-2025
University Medical Center Hamburg-Eppendorf
2024-2025
Universität Hamburg
2024-2025
Roche (Switzerland)
2011-2024
Carol Davila University of Medicine and Pharmacy
2024
Johns Hopkins Medicine
2022
Georgetown University
2022
Charité - Universitätsmedizin Berlin
1995-2021
AxoSim (United States)
2021
Lipoteichoic acid (LTA) derived fromStreptococcus pneumoniae, purified employing a chloroform/methanol protocol, and from Staphylococcus aureus, prepared by the recently described butanol extraction procedure, was investigated regarding its interaction with lipopolysaccharide (LPS)-binding protein (LBP), CD14, Toll-like receptors (TLRs)-2 -4, MD-2. LTA both organisms induced cytokine synthesis in human mononuclear phagocytes. Activation LBP- CD14-dependent, formation of complexes LBP soluble...
Lipopolysaccharides (LPS) are cell-surface components of Gram-negative bacteria and microbe-/pathogen-associated molecular patterns in animal pathosystems. As for plants, the mechanisms signal transduction response to LPS not known. Here, we show that Arabidopsis thaliana reacts with a rapid burst NO, hallmark innate immunity animals. Fifteen preparations (among them Burkholderia cepacia, Pseudomonas aeruginosa , Erwinia carotovora ) as well lipoteichoic acid from Gram-positive...
Abstract Toll-like receptor (TLR) 2 and TLR4 play a pivotal role in recognition of Candida albicans. We demonstrate that TLR2−/− mice are more resistant to disseminated infection, this is associated with increased chemotaxis enhanced candidacidal capacity macrophages. Although production the proinflammatory cytokines TNF, IL-1α, IL-1β normal, IL-10 release severely impaired mice. This accompanied by 50% decrease CD4+CD25+ regulatory T (Treg) cell population In vitro studies confirmed...
Abstract Many drugs have progressed through preclinical and clinical trials been available – for years in some cases before being recalled by the FDA unanticipated toxicity humans. One reason such poor translation from drug candidate to successful use is a lack of model systems that accurately recapitulate normal tissue function human organs their response compounds. Moreover, tissues body do not exist isolation, but reside highly integrated dynamically interactive environment, which actions...
Lipoteichoic acids (LTAs) have been proposed as putative Gram-positive immunostimulatory counterparts to Gram-negative lipopolysaccharides. However, LTA from Staphylococcus aureus, the clinically most frequent pathogen, was inactive after purification. Here, a novel isolation procedure prepare pure (>99%) biologically active LTA, allowing first structural analysis by nuclear magnetic resonance and mass spectrometry, is described. A comparison with purified standard techniques revealed that...
Teichoic acids (TAs), and especially lipoteichoic (LTAs), are one of the main immunostimulatory components pathogenic Gram-positive bacteria. Their contribution to immunomodulatory properties commensal bacteria lactic acid has not yet been investigated in detail. To evaluate role TAs interaction between immune system, we analyzed antiinflammatory a mutant Lactobacillus plantarum NCIMB8826 affected TA biosynthesis pathway both vitro (mononuclear cells stimulation) vivo (murine model colitis)....
The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, constituent atherogenic lipoproteins, binds and induces clustering co-receptors rafts. In resting cells, was associated with CD55, Fcγ-receptors CD32 CD64 pentaspan CD47. Ceramide further recruited complement 3 (CD11b/CD18) CD36 into proximity CD14. Lipopolysaccharide, addition, induced co-clustering Toll-like 4,...
The maintenance of high standards is fundamental to all good scientific practice, and essential for maximising the reproducibility, reliability, credibility, acceptance proper application any results produced. aim this Guidance on Good Cell Culture Practice (GCCP) promote these reduce uncertainty in development animal human cell tissue culture procedures products, by encouraging greater international harmonisation, rationalisation standardisation laboratory practices, quality control...
Chemical regulation is challenged by the large number of chemicals requiring assessment for potential human health and environmental impacts. Current approaches are too resource intensive in terms time, money animal use to evaluate all under development or already on market. The need timely robust decision making demands that regulatory toxicity testing becomes more cost-effective efficient. One way realize this goal being strategic directing resources; focusing highest concern, limiting...
Earlier we created a chemical hazard database via natural language processing of dossiers submitted to the European Chemical Agency with approximately 10 000 chemicals. We identified repeat OECD guideline tests establish reproducibility acute oral and dermal toxicity, eye skin irritation, mutagenicity sensitization. Based on 350-700+ chemicals each, probability that an animal test would output same result in was 78%-96% (sensitivity 50%-87%). An expanded more than 866 properties/hazards used...
Targeting glutamine metabolism via pharmacological inhibition of glutaminase has been translated into clinical trials as a novel cancer therapy, but available drugs lack optimal safety and efficacy. In this study, we used proprietary emulsification process to encapsulate bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl)ethyl sulfide (BPTES), selective relatively insoluble inhibitor, in nanoparticles. BPTES nanoparticles demonstrated improved pharmacokinetics efficacy compared with...
Recent advances in human stem cell-derived brain organoids promise to replicate critical molecular and cellular aspects of learning memory possibly cognition vitro . Coining the term “organoid intelligence” (OI) encompass these developments, we present a collaborative program implement vision multidisciplinary field OI. This aims establish OI as form genuine biological computing that harnesses using scientific bioengineering an ethically responsible manner. Standardized, 3D, myelinated can...
Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording stimulation activity from electrogenic cells offer notable potential interrogating brain organoids. However, conventional MEAs, initially designed monolayer cultures, limited contact area restricted to bottom 3D Inspired by shape electroencephalography caps, we developed miniaturized wafer-integrated MEA caps...