A5032637422- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Antioxidant Activity and Oxidative Stress
- Free Radicals and Antioxidants
- Fatty Acid Research and Health
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammatory mediators and NSAID effects
- Edible Oils Quality and Analysis
- Peroxisome Proliferator-Activated Receptors
- Amyotrophic Lateral Sclerosis Research
- Electron Spin Resonance Studies
- Redox biology and oxidative stress
- Gout, Hyperuricemia, Uric Acid
- Trypanosoma species research and implications
- Cholesterol and Lipid Metabolism
- Parkinson's Disease Mechanisms and Treatments
- Sulfur Compounds in Biology
- Research on Leishmaniasis Studies
- Estrogen and related hormone effects
- Immune Response and Inflammation
- Genomics, phytochemicals, and oxidative stress
- Alcohol Consumption and Health Effects
- bioluminescence and chemiluminescence research
- Metal-Catalyzed Oxygenation Mechanisms
- Endoplasmic Reticulum Stress and Disease
Universidad de la República
2015-2024
Universidad de Montevideo
1990-2017
Instituto de Química y Fisicoquímica Biológicas
2011
Centro Uruguayo de Imagenología Molecular
2010
Cardiff University
2007
University of Pittsburgh
2007
Universidad La República
2006
Universidade de São Paulo
2002
University of Alabama at Birmingham
1994-2000
Superoxide (O2-.), nitric oxide (.NO), and their reaction product peroxynitrite (ONOO-) have all been shown to independently exert toxic target molecule reactions. Because these reactive species are often generated in excess during diverse inflammatory other pathologic circumstances, we assessed the influence of .NO on membrane lipid peroxidation induced by O2-., H2O2, .OH derived from xanthine oxidase (XO) ONOO-. Experimental conditions oxidation systems were adjusted yield different rates...
Peroxynitrite (ONOO-), the reaction product of superoxide (O2•-) and nitric oxide (•NO), nitrates tyrosine other phenolics. We report herein that tryptophan is also nitrated by peroxynitrite in absence transition metals to one predominant isomer nitrotryptophan, as determined from spectral characteristics liquid chromatography−mass spectrometry analysis. At high ratios, oxidation products were detected well. The amount nitrotryptophan formed increased at acidic pH, with an apparent pKa 7.8....
Extra virgin olive oil (EVOO) and olives, key sources of unsaturated fatty acids in the Mediterranean diet, provide health benefits to humans. Nitric oxide (•NO) nitrite (NO2−)-dependent reactions yield electrophilic nitroalkene derivatives (NO2-FA) that manifest salutary pleiotropic cell signaling responses mammals. Herein, endogenous presence NO2-FA both EVOO fresh olives was demonstrated by mass spectrometry. The nature these species affirmed detection significant levels protein cysteine...
Resolution of inflammation is an active and regulated process that leads to the clearance cell debris immune cells from challenged tissue, facilitating recovery homeostasis. This physiological response coordinated by endogenous bioactive lipids known as specialized pro-resolving mediators (SPMs). When resolution fails, becomes uncontrolled leading chronic tissue damage, occurs in multiple sclerosis (MS).SPMs key biosynthetic enzymes involved SPM production were analysed metabololipidomics...
The reactions of nitric oxide (<sup>⋅</sup>NO) and α-tocopherol (α-TH) during membrane lipid oxidation were examined compared with the pair α-TH/ascorbate. Nitric serves as a more potent inhibitor peroxidation propagation than α-TH protects from oxidation. Mass spectrometry, oxygen <sup>⋅</sup>NO consumption, conjugated diene analyses, fluorescence determinations all demonstrated that preferentially reacts radical species, consumption not occurring until concentrations fell below critical...
Nitric oxide (*NO) has been proposed to play a relevant role in modulating oxidative reactions lipophilic media like biomembranes and lipoproteins. Two factors that will regulate *NO reactivity the lipid milieu are its diffusion solubility, but there is no data concerning actual (D) partition coefficients (KP) of biologically hydrophobic phases. Herein, "equilibrium-shift" method was designed directly determine O2 liposomes low density lipoprotein (LDL) relative water. It found partitions...
Nitric oxide (*NO) is a pervasive free radical species that concentrates in lipophilic compartments to serve as potent inhibitor of lipid and low-density lipoprotein oxidation processes. In this study, we synthesized, characterized, detected nitrated derivatives linoleic acid (18:2) human blood plasma using high-pressure liquid chromatography coupled with electrospray ionization tandem mass spectrometry. While the reaction nitronium tetrafluoroborate 18:2 presented ions mass/charge (m/z)...
Nitrated fatty acids (nitroalkenes) have been recently detected and quantified in cell membranes human plasma. However, nitration of arachidonate (AA), that could redirect AA-dependent signaling pathways, has not studied detail. Herein, we synthesized determined for the first time isomer distribution nitroarachidonate (AANO2) demonstrate its ability to modulate inflammation. Synthesis AANO2 was achieved by AA treatment with sodium nitrite acidic conditions following HPLC separation. Mass...
Nitroalkene derivatives of fatty acids act as adaptive, anti-inflammatory signalling mediators, based on their high-affinity PPARgamma (peroxisome-proliferator-activated receptor gamma) ligand activity and electrophilic reactivity with proteins, including transcription factors. Although free or esterified lipid nitroalkene have been detected in human plasma urine, generation by inflammatory stimuli has not reported. In the present study, we show increased nitration cholesteryl-linoleate...
Nitro-fatty acids (NO2-FA) are electrophilic lipid mediators derived from unsaturated fatty acid nitration. These species produced endogenously by metabolic and inflammatory reactions mediate anti-oxidative anti-inflammatory responses. NO2-FA have been postulated as partial agonists of the Peroxisome Proliferator-Activated Receptor gamma (PPARγ), which is predominantly expressed in adipocytes myeloid cells. Herein, we explored molecular cellular events associated with PPARγ activation...