A5015758572- Marine Toxins and Detection Methods
- Eicosanoids and Hypertension Pharmacology
- Environmental Toxicology and Ecotoxicology
- Marine and coastal ecosystems
- Marine Bivalve and Aquaculture Studies
- Neuroscience of respiration and sleep
- Marine Biology and Environmental Chemistry
- Nicotinic Acetylcholine Receptors Study
- Peroxisome Proliferator-Activated Receptors
- Fatty Acid Research and Health
- Sphingolipid Metabolism and Signaling
- Reproductive biology and impacts on aquatic species
- Antiplatelet Therapy and Cardiovascular Diseases
- Inflammatory mediators and NSAID effects
- Environmental Chemistry and Analysis
- Immune Cell Function and Interaction
- Hormonal and reproductive studies
- Immune cells in cancer
- Aquatic Ecosystems and Phytoplankton Dynamics
- Alcohol Consumption and Health Effects
- Zoonotic diseases and public health
- Cholesterol and Lipid Metabolism
- Forensic Toxicology and Drug Analysis
- Pharmacological Effects and Assays
- Immune Response and Inflammation
Centre for Environment, Fisheries and Aquaculture Science
2016-2025
Lowestoft Hospital
2023
University of the Highlands and Islands
2012-2016
Cardiff University
2007-2016
Millennium Institute
2012
Centre for Inflammation Research
2011
University of Edinburgh
2011
Tenovus Cancer Care
2009
Inflammation is a physiological response to tissue trauma or infection, but leukocytes, which are the effector cells of inflammatory process, have powerful remodelling capabilities. Thus, ensure their precise localisation, passage leukocytes from blood into inflamed tightly regulated. Recruitment borne neutrophils stroma occurs during early inflammation. In this peptide agonists chemokine family assumed provide chemotactic stimulus capable supporting migration across vascular endothelial...
Abstract Background: An ultrahigh-performance LC (UHPLC)–tandem MS (MS/MS) method for determination of paralytic shellfish poisoning toxins and tetrodotoxin (TTX) in bivalve molluscs was developed. To be used regulatory testing, it needed to validated through collaborative study. Objective: The aim conduct a study with 21 laboratories, using results assess performance. Methods: Study materials incorporated species mussels, oysters, cockles, scallops, clams were assessed demonstrate stability...
Paretic and paralyzing syndromes affecting wild birds are widely described in the literature, with outbreaks showing an increase frequency intensity worldwide during recent years. In Iberian Peninsula, a paretic clinical picture without known etiology mostly gulls has been reported last few decades. This syndrome (PS) affects waterbirds is characterized by set of signs ascendent flaccid paralysis, dyspnea, diarrhea at different levels severity. study presents first macro-analysis some...
12/15-Lipoxygenase (LOX) mediates immune-regulatory activities not accounted for by its known free acid eicosanoids, suggesting that additional lipids may be generated activated cells. To characterize novel LOX-derived lipids, a lipidomic approach was utilized. Ionophore-activated interleukin-4-treated human peripheral monocytes up to 10-fold more esterified 15-hydroxyeicosatetraenoic (15-HETE) than in phosphatidylinositol 3-kinase- and protein kinase C-sensitive manner. Precursor scanning...
VOLUME 282 (2007) PAGES 20151–20163 In our article, we stated, “Comparison of levels free 15-HETE after hydrolysis either total lipid extract (Fig. 1) or purified monocyte PE 3) shows that the PE-esterified HETE does not seem to account for all esterified formed. Specifically, varied from 100 2000 ng/4 × 106 cells (due donor variation), but only 17.5 was detected purification and one donor. This suggests is into additional nonphospholipid pools have been here, although perhaps being ionized...
Alzheimer's disease (AD) is of major concern in ageing populations and we have used the Tg2576 mouse model to understand connections between brain lipids amyloid pathology. Because dietary docosahexaenoic acid (DHA) has been identified as beneficial, compared mice fed with a DHA-supplemented diet those on nutritionally-sufficient diet. Major phospholipids from cortex, hippocampus cerebellum were separated analysed. Each phosphoglyceride had characteristic fatty composition which was similar...
Abstract Intricate links between aquatic animals and their environment expose them to chemical pathogenic hazards, which can disrupt seafood supply. Here we outline a risk schema for assessing potential impacts of microbial hazards on discrete subsectors aquaculture—and control measures that may protect As national governments develop strategies achieve volumetric expansion in production from aquaculture meet increasing demand, propose an urgent need simultaneous focus controlling those...
Abstract The peritoneal macrophage (Mφ) is the site of greatest 12/15-lipoxygenase (12/15-LOX) expression in mouse; however, its immunoregulatory role this tissue has not been explored. Herein, we show that 12/15-LOX expressed by 95% resident CD11bhigh cells, with remaining 5% being 12/15-LOX−. 12/15-LOX+ cells are phenotypically defined high F4/80, SR-A, and Siglec1 expression, enhanced IL-10 G-CSF generation. In contrast, 12/15-LOX− a dendritic cell population. Resident Mφ numbers were...
In this study, murine peritoneal macrophages from naïve lavage were found to generate four phospholipids that contain 12-hydroxyeicosatetraenoic acid (12-HETE). They comprise three plasmalogen and one diacyl phosphatidylethanolamines (PEs) (16:0p, 18:1p, 18:0p, 18:0a at sn-1) are absent in 12/15-lipoxygenase (12/15-LOX)-deficient mice. generated acutely response calcium mobilization, primarily cell-associated, detected on the outside of plasma membrane. Levels 12-HETE-PEs a similar range...
Three people in England consumed fish steaks labeled as Red Snapper (Lutjanus bohar) originating from the Indian Ocean. Within 12 h, all three experienced sickness including nausea, vomiting, diarrhea, well myalgia and paresthesia. a single package of obtained grocery store were consumed, leaving one uneaten, which was submitted for analysis. Cytotoxicity testing via mouse neuroblastoma assay confirmed presence sodium channel specific activity consistent with ciguatoxin standard, levels...
Microalgae form the basis of marine food webs, essential in sustaining top predators including seabirds. However, certain species microalgae synthesize biotoxins, which can accumulate shellfish and fish may cause harm to animals feeding on them. Toxins produced by dinoflagellates have been previously observed be poisonous Also, freshwater brackish habitats, cyanobacteria caused bird mortality events. In this work, we analyze prevalence six families biotoxins (paralytic toxins (PSTs),...
Members of the benthic marine dinoflagellate genus Amphidinium produce a variety bioactive compounds, exhibiting potent cytotoxicity in cell assays. Crude methanolic extracts from three genetically distinct cultured strains A. eilatiense J.J. Lee were screened for against human breast and four lung cancer lines to evaluate potential applications anticancer therapy. A standard tetrazolium viability assay demonstrated that crude extract (100 µg mL−1) strain AeSQ181 reduced by 20–35% five...
The metabolism of bromine in marine brown algae remains poorly understood. This contrasts with the recent finding that accumulation iodide alga Laminaria serves provision an inorganic antioxidant – first case documented from a living system. aim this study was to use interdisciplinary array techniques chemical speciation, transformation, and function investigate link between iodine metabolism, particular context. First, levels different tissues were compared by inductively coupled plasma MS....
Fenretinide (FEN) is a synthetic retinoid that inhibits obesity and insulin resistance in high-fat diet (HFD)-fed mice completely prevents 3T3-L1 pre-adipocyte differentiation. The aim of this study was to determine the mechanism(s) FEN action adipocytes mice. We used model adipogenesis, fully differentiated adipose tissue from HFD-induced obese investigate mechanisms action. measured expression adipogenic genes by qPCR activation nutrient-signalling pathways western blotting. Global lipid...