A5011917504- Marine Toxins and Detection Methods
- Marine and coastal ecosystems
- Nicotinic Acetylcholine Receptors Study
- Neuroscience of respiration and sleep
- Forensic Toxicology and Drug Analysis
- Environmental Chemistry and Analysis
- Microbial Community Ecology and Physiology
- Environmental Toxicology and Ecotoxicology
- Photoreceptor and optogenetics research
- Analytical chemistry methods development
- Protist diversity and phylogeny
- Marine Biology and Environmental Chemistry
- Aquatic Ecosystems and Phytoplankton Dynamics
- Oxidative Organic Chemistry Reactions
- Animal Ecology and Behavior Studies
- Molecular Sensors and Ion Detection
- Mass Spectrometry Techniques and Applications
- Folate and B Vitamins Research
- Biochemical and biochemical processes
- Hemoglobin structure and function
- Cassava research and cyanide
- Plant-based Medicinal Research
- Nuts composition and effects
- Probiotics and Fermented Foods
- Regulation of Appetite and Obesity
Universidade de Vigo
2003-2023
Universidade de Santiago de Compostela
2022
Wageningen University & Research
2019
Cawthron Institute
2019
Centre for Environment, Fisheries and Aquaculture Science
2014
Canadian Food Inspection Agency
2014
Institute for Marine Biosciences
1999
Abstract Background: An ultrahigh-performance LC (UHPLC)–tandem MS (MS/MS) method for determination of paralytic shellfish poisoning toxins and tetrodotoxin (TTX) in bivalve molluscs was developed. To be used regulatory testing, it needed to validated through collaborative study. Objective: The aim conduct a study with 21 laboratories, using results assess performance. Methods: Study materials incorporated species mussels, oysters, cockles, scallops, clams were assessed demonstrate stability...
Tetrodotoxins (TTX) are a potent group of natural neurotoxins putatively produced by symbiotic microorganisms and affecting the aquatic environment. These have been recently found in some species bivalves gastropods along European Coasts (Greece, UK, The Netherlands) linked to presence high concentrations Vibrio, particular Vibrio parahaemolyticus. This study is focused on evaluation TTX (mussels, oysters, cockles, clams, scallops, razor clams) from Galician Rias (northwest Spain). detection...
Ciguatoxins (CTXs), endemic from tropical and subtropical regions of the Pacific Indian Ocean Caribbean Sea, have caused several human poisonings during last decade in Europe. Ciguatera fish (CFP) Madeira Canary Islands appear to be particularly related with consumption caught close Selvagens Islands, a Portuguese natural reserve composed three small islands that harbor high biomass. In this study, specimens considered as potential vectors CTXs were archipelagos for toxins determination via...
Ciguatera Fish Poisoning is a worldwide concern caused by the consumption of fish contaminated with ciguatoxins not only in endemic regions Pacific Ocean or Caribbean Sea but also emerging areas Macaronesia on eastern Atlantic. The recent emergence these toxins other coastal worldwide, prompted need for characterization risk areas. This has been recently identified as potential threat subtropical Atlantic coast and scientific efforts are being focused identification confirmation involved...
Over the last decade, knowledge has significantly increased on taxonomic identity and distribution of dinoflagellates genera Gambierdiscus Fukuyoa. Additionally, a number hitherto unknown bioactive metabolites have been described, while role these compounds in ciguatera poisoning (CP) remains to be clarified. Ciguatoxins maitotoxins are very toxic produced by described since 1980s. generally as main contributors this food intoxication. Recent reports CP temperate waters Canary Islands...
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) main toxin causing CP through caught in Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed initial confirmation presence C-CTX1 samples from abovementioned...
The Selvagens Islands, which are a marine protected area located at the southernmost point of Portuguese maritime zone, have been associated with fish harboring ciguatoxins (CTX) and linked to ciguatera poisonings. This study reports results field sampling campaign carried out in September 2018 these remote rarely surveyed islands. Fifty-six specimens from different trophic levels were caught for CTX-like toxicity determination by cell-based assay (CBA) toxin content analysis liquid...
Abstract This paper shows the results of an intralaboratory validation a fast method for determination lipophilic shellfish toxins working under acidic conditions using ultra-high performance LC (UHPLC) with MS/MS. Fourteen marine and domoic acid were acquired polarity switching.Whereas azaspiracids (AZAs), pecenotoxins, 13-desmethyl spirolide C (SPX1), gymnodimine analyzed in positive mode, yessotoxins (YTXs) measured negative mode. The okadaic (OA) group compounds both negativeionization...
Tetrodotoxin (TTX) is a potent neurotoxin, considered an emerging toxin in Europe where recently safety limit of 44 µg TTX kg
Cyclic imines (CIs) are being considered as emerging toxins in the European Union, and a scientific opinion has been published by Food Safety Authority (EFSA) which an assessment of risks to human health related their consumption carried out. Recommendations on EFSA include search for data occurrence CIs shellfish using confirmatory methods liquid chromatography-tandem mass spectrometry (LC-MS/MS), need be developed optimized. The aim this work is application LC-MS/MS analysis gymnodimines...
Abstract Liquid chromatography (LC) coupled to mass spectrometry (MS) is being widely applied as an analytical tool in the field of marine biotoxins both for regulated and new emerging compounds. LC‐MS/MS recently became reference method control lipophilic toxins European Union, methods are developed optimized extend applicability this technique other toxin groups. In work, conditions analysis ciguatoxins (CTXs) by were investigated using standard solutions CTX1B CTX3C, which structurally...
Cobalamin (B12) is an essential cofactor that exclusively synthesized by some prokaryotes while many and eukaryotes require external supply of B12. The spatial temporal availability B12 poorly understood in marine ecosystems. Field measurements along with a large set ancillary biotic abiotic factors were obtained during three oceanographic cruises the NW Iberian Peninsula, covering different scales. concentrations remarkably low (<1.5 pM) all samples, being significantly higher at subsurface...
Dinoflagellate species of the genera Gambierdiscus and Fukuyoa are known to produce ciguatera poisoning-associated toxic compounds, such as ciguatoxins, or other toxins, maitotoxins. However, many strains remain poorly characterized in areas where they were recently identified, western Mediterranean Sea. In previous studies carried out by our research group, a G. australes strain from Balearic Islands (Mediterranean Sea) presenting MTX-like activity was LC-MS/MS LC-HRMS detecting 44-methyl...
The EuroCigua project main objective is to characterize the risk of Ciguatera Poisoning (CP) in Europe including several specific objectives: determine incidence ciguatera and epidemiological characteristics cases; assess presence ciguatoxin food environment develop validate methods for detection, quantification confirmation contaminated specimens. This report compiles activities carried out during from signing April 2016 until December 2020. present document corresponds Deliverable No. 6:...
Abstract Background Given the recent detection of tetrodotoxin (TTX) in bivalve molluscs but absence a full collaborative validation study for TTX determination large number shellfish samples, interlaboratory assessment method performance was required to better understand current capabilities accurate and reproducible quantitation using chemical immunoassay methods. Objective The aim conduct an with multiple laboratories, results assess acceptability different testing Methods Homogenous...
The biodiversity of lactic acid bacteria in musts and wines Albariño variety has been studied. identification species was addressed through a combination biochemical genetic methods (API® 50 CHL test, 16S rDNA recA gene sequences, Amplified Ribosomal DNA Restriction Analysis -ARDRA- 16S-26S intergenic region analysis). results grouped the isolates into six predominating those genus Lactobacillus showing typical biogeographical distribution. Among sixteen strains evaluated, eight them showed...
Ciguatoxins (CTXs) are naturally occurring neurotoxins that can accumulate in fish and cause Ciguatera Poisoning (CP) seafood consumers. Ciguatoxic have been detected tropical subtropical regions of the world including Pacific Indian Oceans, Caribbean Sea, more recently northeast Atlantic Ocean. The biogeographic distribution ciguatoxic appears to be expanding; however, paucity CTX standards reference materials limits ability public health authorities monitor for these toxins supply chains....
The recent emergence of ciguatera in the eastern Atlantic, particularly Canary Islands (Spain) and Madeira (Portugal) prompted development implementation liquid chromatography tandem-mass spectrometry (LC/MS-MS) methods for detection ciguatoxins fish. complexity fish tissue matrices, low concentrations hazardous fish, scarcity ciguatoxin standards present challenging issues successful routine analysis. A laboratory reference material Caribbean Ciguatoxin-1 (C-CTX1), which was previously...
Abstract An interlaboratory ring trial was designed and conducted by the Centre for Environment, Fisheries, Aquaculture Science to investigate a range of issues affecting analysis candidate Pacific oyster paralytic shellfish toxin reference material. A total 21 laboratories participated in study supplied results using one or more three instrumental methods, specifically precolumn oxidation (Pre-COX) LC with fluorescence detection (FLD; AOAC Official Method 2005.06), postcolumn (PCOX) LC-FLD...
Harmful algal blooms (HABs) have been increasingly recorded over the last decades and much work has linked these events to multiple oceanographic climate disturbances. HABs can affect ecosystems either as that dissolved oxygen, clog fish gills, or smother corals through production of biotoxins which living marine resources food web transfers aerosols. HAB represent a natural driver decline potential extinction aquatic organisms, from invertebrates mammals, may offer little evolutionary...
In mammals ceramides are involved in the modulation of orexigenic effects ghrelin (GHRL). We previously demonstrated rainbow trout that intracerebroventricular (ICV) treatment with ceramide (2.5 µg/100g fish) resulted an anorexigenic response, i.e. a response opposed to described where is orexigenic. Therefore, we hypothesize putative interaction between GHRL and must be different fish. Accordingly, first experiment observed levels hypothalamus did not change after ICV GHRL. second...