A5068146694- Marine Toxins and Detection Methods
- Nicotinic Acetylcholine Receptors Study
- Marine Biology and Environmental Chemistry
- Protist diversity and phylogeny
- Environmental Chemistry and Analysis
- Biocrusts and Microbial Ecology
- Chemical synthesis and alkaloids
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microtubule and mitosis dynamics
- Marine and coastal ecosystems
- Neuroscience of respiration and sleep
- Ion channel regulation and function
- Hemoglobin structure and function
- Marine Sponges and Natural Products
- Nitric Oxide and Endothelin Effects
- Neurotransmitter Receptor Influence on Behavior
- Autophagy in Disease and Therapy
- Bioactive Compounds and Antitumor Agents
- Synthesis of heterocyclic compounds
- Analytical chemistry methods development
- Venomous Animal Envenomation and Studies
- Seaweed-derived Bioactive Compounds
- Analytical Methods in Pharmaceuticals
- Synthesis and Biological Activity
- Endoplasmic Reticulum Stress and Disease
Cawthron Institute
2019
Universidade de Santiago de Compostela
2006-2016
Japan Food Research Laboratories
2009
University of California, San Diego
2006-2008
Scripps Research Institute
2006-2008
Istituto di Farmacologia Traslazionale
2006
Abstract Background: An ultrahigh-performance LC (UHPLC)–tandem MS (MS/MS) method for determination of paralytic shellfish poisoning toxins and tetrodotoxin (TTX) in bivalve molluscs was developed. To be used regulatory testing, it needed to validated through collaborative study. Objective: The aim conduct a study with 21 laboratories, using results assess performance. Methods: Study materials incorporated species mussels, oysters, cockles, scallops, clams were assessed demonstrate stability...
Tetrodotoxin (TTX) is starting to appear in molluscs from the European waters and a hazard seafood consumers. This toxin blocks sodium channels resulting neuromuscular paralysis even death. As part of risk assessment process leading safe level for TTX, oral toxicity data are required. In this study, 4-level Up Down Procedure was designed order determine first time lethal dose 50 (LD50) No Observed Adverse Effect Level (NOAEL) mice by using an accurate well-characterized TTX standard.
Azaspiracid-1 (AZA-1) is a marine toxin discovered 10 years ago. Since then, toxicologic studies have demonstrated that AZA-1 targets several organs in vivo, including the intestine, lymphoid tissues, lungs, and nervous system; however, mechanism of action remains unknown. Studies vitro suggest affects actin cytoskeleton nonadherent cells. We characterized effects on adherent cells cell growth, an adhesion-dependent process many types, analyzed structure dependency this toxicity. Confocal...
Palytoxin is one of the most complex and biggest molecules known to show extreme acute toxicity. The dinoflagellate Ostreopsis spp., producer organism palytoxin, has been shown be distributed worldwide, thus making palytoxin an emerging toxin. Rat-derived hepatocytes (Clone 9) BE (2)-M17 human neuroblastoma cells were used test or palytoxin-like compounds by measuring cell metabolic rate with Alamar Blue. dose-dependent decrease in viability was specifically inhibited ouabain case cells....
Okadaic acid (OA) and microcystins (MCs) are structurally different toxins with the same mechanism of action, inhibition serine/threonine protein phosphatases (PPs). Methyl okadaate (MeOk), a methyl ester derivative OA, was considered almost inactive due to its weak PP1 PP2A. Here, we have investigated activity potency MeOk in hepatic cells comparison that OA MCs.We tested effects MeOK, microcystin-leucine arginine (MC-LR) on metabolic rate, actin cytoskeleton glucose uptake rat hepatocyte...
Background: Gambierol is a polycyclic ether toxin with the same biogenetic origin as ciguatoxins. has been associated neurological symptoms in humans even though its mechanism of action not fully characterized. Methods: We studied effect gambierol human neuroblastoma cells by using bis-oxonol to measure membrane potential and FURA-2 monitor intracellular calcium. Results: found that this toxin: i) produced depolarization, ii) potentiated veratridine on iii) decreased ciguatoxininduced...
Hapalindoles make up a large group of bioactive metabolites the cyanobacterial order Stigonematales. 12-epi-Hapalindole E isonitrile, 12-epi-hapalindole C J and hapalindole L from Fischerella are acutely toxic for insect larvae; however, biochemical targets responsible biological activities hapalindoles not understood. We describe here electron impact mass spectra these four congeners; their structures were confirmed by nuclear magnetic resonance spectroscopy. In combination with presented...
Abstract Background Given the recent detection of tetrodotoxin (TTX) in bivalve molluscs but absence a full collaborative validation study for TTX determination large number shellfish samples, interlaboratory assessment method performance was required to better understand current capabilities accurate and reproducible quantitation using chemical immunoassay methods. Objective The aim conduct an with multiple laboratories, results assess acceptability different testing Methods Homogenous...
Background and purpose. Pectenotoxins are macrocyclic lactones found in dinoflagellates of the genus Dinophysis , which induce severe liver damage mice after i.p. injection. Here, we have looked for mechanism(s) underlying this hepatotoxicity. Experimental approach. Effects pectenotoxin (PTX)‐1, PTX‐2, PTX‐2 seco acid (PTX‐2SA) PTX‐11 were measured a hepatocyte cell line with cancer characteristics (Clone 9) primary cultures rat hepatocytes. Cell morphology was assessed by confocal...
Background and purpose: Dinoflagellates from the genus Ostreopsis have been related to production of palytoxin analogues. Based on that, this paper describes functional studies crude extracts cf. siamensis collected in Mediterranean Sea order biochemically characterize their toxic compounds. Methods: We compared effects 5 dinoflagellates with a commercially available purified ovata extract metabolic activity, membrane potential, cytosolic calcium levels by using fluorescent dyes. Results:...
Background: Polycavernoside A is a glycosidic marine toxin first extracted from the red alga Polycavernosa tsudai in 1991 when 3 people died after ingestion of this food. an interesting molecule because its complex macrolide structure and strong bioactivity. However, target site has not been characterized. Methods: We studied effects synthethic analog polycavernoside on human neuroblastoma cells by measuring changes membrane potential with bis-oxonol variations intracellular calcium levels...
Methyl okadaate is a derivative of the lipophilic polyether okadaic acid (OA), well‐known inducer apoptosis. OA inhibits Ser/Thr protein phosphatases (PPs), among them types 1 and 2A (PP1 PP2A), whereas methyl lacks PP1/PP2A inhibitory activity in vitro . As progressive loss neuronal cytoarchitecture major event that precedes death, this work we studied comparatively effects both toxins on actin cytoskeleton organization human neuroblastoma cells by filamentous (F‐actin) labeling with...
The azaspiracids are a group of marine toxins recently described that currently includes 20 analogues. Not much is known about their mechanism action, although effects on some cellular functions have been found in vitro. We used the reported cell viability, actin cytoskeleton, and caspase activation to study structure−activity relationship AZA-1 AZA-2 role carboxylic acid moiety toxicity. AZA-1, AZA-2, synthetic AZA-2-methyl ester (AZA-2-ME), where C1 was esterified corresponding methyl...
Ostreocin-D, discovered in the past decade, is a marine toxin produced by dinoflagellates. It shares structure with palytoxin, toxic compound responsible for seafood intoxication named clupeotoxism. At cellular level, action sites and pharmacological effects ostreocin-D are still almost unknown. Previously, we demonstrated that these toxins change filamentous actin cytoskeleton, which essential multiple functions. However, nothing has yet been reported about what happens unpolymerized pool....
Many microalgae produce compounds that exhibit potent biological activities. Ingestion of marine organisms contaminated with those toxins results in seafood poisonings. In many cases, the lack toxic material turns out to be an obstacle make toxicological investigations needed. this study, we evaluate cytotoxicity several on neuroblastoma cells, focusing gambierol and its effect cytosolic calcium levels. addition, compared effects toxin ciguatoxin, brevetoxin, gymnocin-A, which shares a...