A5049489690- Marine Toxins and Detection Methods
- Nicotinic Acetylcholine Receptors Study
- Crystallization and Solubility Studies
- Surfactants and Colloidal Systems
- X-ray Diffraction in Crystallography
- Drug Solubulity and Delivery Systems
- Analytical Chemistry and Chromatography
- Environmental Chemistry and Analysis
- Drug Transport and Resistance Mechanisms
- Neuroscience of respiration and sleep
- Molecular Sensors and Ion Detection
- Supramolecular Chemistry and Complexes
- Marine Biology and Environmental Chemistry
- Forensic Toxicology and Drug Analysis
- Supramolecular Self-Assembly in Materials
- Environmental Toxicology and Ecotoxicology
- Dendrimers and Hyperbranched Polymers
- bioluminescence and chemiluminescence research
- Mesoporous Materials and Catalysis
- Covalent Organic Framework Applications
- Advanced Drug Delivery Systems
- Fluorine in Organic Chemistry
- Photoreceptor and optogenetics research
- Estrogen and related hormone effects
- Alzheimer's disease research and treatments
Universidade de Santiago de Compostela
2007-2014
Institute of Clinical and Experimental Medicine
2009
Pontificia Universidad Católica de Chile
2009
Northeast Ohio Medical University
2009
Ohio University
2009
Universidad de Salamanca
2009
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2009
Abstract Background: An ultrahigh-performance LC (UHPLC)–tandem MS (MS/MS) method for determination of paralytic shellfish poisoning toxins and tetrodotoxin (TTX) in bivalve molluscs was developed. To be used regulatory testing, it needed to validated through collaborative study. Objective: The aim conduct a study with 21 laboratories, using results assess performance. Methods: Study materials incorporated species mussels, oysters, cockles, scallops, clams were assessed demonstrate stability...
Tetrodotoxin (TTX) is starting to appear in molluscs from the European waters and a hazard seafood consumers. This toxin blocks sodium channels resulting neuromuscular paralysis even death. As part of risk assessment process leading safe level for TTX, oral toxicity data are required. In this study, 4-level Up Down Procedure was designed order determine first time lethal dose 50 (LD50) No Observed Adverse Effect Level (NOAEL) mice by using an accurate well-characterized TTX standard.
Gambierdiscus species are the producers of marine toxins ciguatoxins and maitotoxins which cause worldwide human intoxications recognized as Ciguatera Fish Poisoning. A deep chemical investigation a cultured strain G. belizeanus, collected in Caribbean Sea, led to identification structural homologue recently described gambierone isolated from same strain. The structure was elucidated mainly by comparison NMR MS data with those ascertained 2D analyses. Gratifyingly, close inspection new...
Spirolides are marine toxins that not currently in the routine monitoring assays. Nicotinic receptors seem to be target of these compounds making them a promising pharmacological tool for related diseases as dementias previously shown vitro. In present work, bioavailability 13-desMethyl spirolide C (13-desMeC) brain and vivo effects were tested. Bioavailability was studied by ultra-performance liquid chromatography-mass spectrometry its effect over Alzheimer hallmarks Proton magnetic...
Paralytic shellfish toxins (PST) traditionally have been analyzed by liquid chromatography with either pre- or post-column derivatization and always a silica-based stationary phase. This technique resulted in different methods that need more than one run to analyze the toxins. Furthermore, tetrodotoxin (TTX) was recently found bivalves of northward locations Europe due climate change, so it is important along PST because their signs toxicity are similar bioassay. The described here detail...
A new gemini steroid surfactant derived from 3alpha,12alpha-dihydroxy-5beta-cholan-24-amine (steroid residue) and ethylenediamintetraacetic acid (spacer) was synthesized characterized in aqueous solution by surface tension, fluorescence intensity of pyrene, light scattering (static dynamic) measurements. These techniques evidence the existence a threshold concentration (cac), below which three layers film is formed at air-water interface. Above cac, two types aggregates--micelles...
Ciguatoxins (CTXs) and maitotoxins (MTXs) are polyether ladder shaped toxins derived from the dinoflagellate Gambierdiscus toxicus. Despite fact that MTXs 3 times larger than CTXs, part of structure resembles CTXs. To date, synthetic ciguatoxin, CTX 3C has been reported to activate voltage-gated sodium channels, whereas main effect MTX is inducing calcium influx into cell leading death. However, there a lack information regarding effects these in common cellular model. Here, order have an...
Abstract Background Given the recent detection of tetrodotoxin (TTX) in bivalve molluscs but absence a full collaborative validation study for TTX determination large number shellfish samples, interlaboratory assessment method performance was required to better understand current capabilities accurate and reproducible quantitation using chemical immunoassay methods. Objective The aim conduct an with multiple laboratories, results assess acceptability different testing Methods Homogenous...
The spirolides and gymnodimines are marine phycotoxins included in the group of cyclic imines. toxicity these compounds to humans is still unknown, although their by intraperitoneal injection rodents very high. A receptor-based method was developed using competition 13-desmethyl spirolide C with biotin-labeled α-bungarotoxin for binding nicotinic acetylcholine receptors immobilization α-bungarotoxin-receptor complex on streptavidin-coated surfaces. quantification immobilized receptor can be...
The formation of supramolecular structures initiated by a p-tert-butylphenylamide derivative deoxycholic acid (Na-t-butPhDC) is investigated. At 1.18 mM concentration Na-t-butPhDC and 37 degrees C, initial flat ribbons are observed which self-transform into helical (with mean pitch angle 47 +/- 6 degrees) finally originate molecular tubes with an external diameter 241 28 nm. Most the show markings value 45 4 degrees, in full agreement predictions simple models based on chiral elastic...
Abstract Hydrophobically modified chitosan containing β‐cyclodextrin (CD) units was synthesized by using tosylated β‐CD. The final product characterized Fourier transform infrared (FTIR) spectroscopy, elemental analysis and TGA, rheometry. polymer bearing β‐CD moieties used to obtain crosslinked microparticles spray‐drying which could then be in a controlled release system for drugs. FTIR confirmed the formation of an amide linkage between cyclodextrin chitosan. As fluorescence spectroscopy...
This study explores the effect of laboratory and industrial steaming on mussels with toxin concentrations above below legal limit. We used mild conditions for steaming, 100 °C 5 min in processing, up to 20 small-scale steaming. Also, we studied heat concentration obtained from two different locations levels dinophysistoxins 3 (DTX3) both mussel matrix pure form (7-O-palmitoyl okadaic ester 7-O-palmytoleyl ester). The results show that loss water due was very small a maximum 9.5%, content...
The reaction of 3β-aminoderivatives cholic and deoxycholic acids (steroid residues) with dimethyl ester ethylenediaminetetraacetic acid (bridge) leads to the formation dimers carrying four carboxylic organic functions, two them located on side chain each steroid residue other bridge. As tetrasodium salts, these new compounds behave as surfactants have been characterized by surface tension, fluorescence intensity pyrene (as a probe), static dynamic light scattering measurements. Thermodynamic...
It is well-known that amines have the tendency to undergo aerial carbonation form sec-ammonium N-carboxylates (carbamates). In fact, carbamate formation may be a quite general and functionally important phenomenon throughout biology, as moieties are key components in biological fixation of CO2 by living organisms. Currently, there renewed interest use amino acids for removal from gas streams also contain oxygen. These facts justify wide research on derivatives carbon dioxide, an aspect this...
Paralytic Shellfish Toxins (PSTs) are polar analytes, most of them with positive charges resulting in a charge-induced dipole at the graphene surface when they approach to it. Graphene is novel material great potentials be used as sorbent due its ultrahigh area. Herein, we perform simulation about retention mechanism PSTs on through Merck Molecular Force Field (MMFF94) minimizations. The overall combination two mechanisms: - Adsorption: strength analyte interactions largely dependent...
A new gemini steroid surfactant derived from 3a,7a,12a-trihydroxy-5ß-cholan-24-amine (steroid residue) and ethylenediamintetraacetic acid (spacer) was synthesized characterized in aqueous solution by surface tension measurements fluorescence intensity of pyrene. These techniques evidence the existence a threshold concentration, cac, below which three layers film is formed at air-water interface. At high concentrations, ratio vibronic peaks pyrene, I1/I3, (= 0.81) very close to published...
Here, we report on the determination of supramolecular adsorption hydroxybenzoate derivates (GC toxins) saxitoxin to a pristine graphene surface using computational method, MMFF94 Force Field implemented in Chem Office package. We use simple model with GC molecule centred surface, simulate interaction stacking system, avoiding edge interactions. find that formation GC–graphene complex is favourable for all molecules. The results our are good agreement chromatographic elution graphite...