A5067887154- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Psoriasis: Treatment and Pathogenesis
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Neuroinflammation and Neurodegeneration Mechanisms
- Autoimmune and Inflammatory Disorders Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Inhalation and Respiratory Drug Delivery
- HIV Research and Treatment
- Lung Cancer Research Studies
- Nanoporous metals and alloys
- HIV/AIDS drug development and treatment
- interferon and immune responses
- EEG and Brain-Computer Interfaces
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Motor Control and Adaptation
- Eosinophilic Esophagitis
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Platelet Disorders and Treatments
Lancaster University Ghana
2024
Manchester Academic Health Science Centre
2020-2023
University of Manchester
2020-2023
Centre for Inflammation Research
2021-2023
Lancaster University
2023
Tetracore (United States)
2021
University of British Columbia
2009-2017
Babraham Institute
2009-2010
University of Surrey
2006
Latent HIV-1 (human immunodeficiency virus-1) provirus is unaffected by current AIDS (acquired syndrome) therapies. We show here that chaetocin, an SUV39H1 histone methyltransferase inhibitor, causes 25-fold induction of latent expression, while producing minimal toxicity and without causing T cell activation. Induction associated with loss H3 lysine 9 (H3K9) trimethylation at the long terminal repeat (LTR) promoter, a corresponding increase in H3K9 acetylation. The effect chaetocin...
Homeostatic control of the immune system involves mechanisms that ensure self-tolerance, survival and quiescence hematopoietic-derived cells. In this study, we demonstrate GTPase immunity associated protein (Gimap)5 regulates these processes in lymphocytes hematopoietic progenitor As a consequence recessive N-ethyl-N-nitrosourea-induced germline mutation P-loop Gimap5, lymphopenia, hepatic extramedullary hematopoiesis, weight loss, intestinal inflammation occur homozygous mutant mice....
The aim of this study was to identify and validate a sensitive, high-throughput, cost-effective SARS-CoV-2 real-time RT-PCR assay be used as surveillance diagnostic tool for in university program. We conducted side-by-side clinical evaluation newly developed multiplex (EZ-SARS-CoV-2 Real-Time RT-PCR) with the commercial TaqPath COVID-19 Combo Kit, which has an Emergency Use Authorization from FDA. EZ-SARS-CoV-2 incorporates two assays targeting N gene, internal control human RNase P PCR...
Psoriasis is a common chronic inflammatory skin disease with no cure. It driven by the interleukin (IL)-23/IL-17A axis and type 17 T helper cells; however, recently, group 3 innate lymphoid cells (ILC3s) have also been implicated. Despite being focus of much research, factors regulating activity ILC3s remain incompletely understood. Immune regulatory pathways are particularly important at barrier sites, such as skin, gut, lungs, which exposed to environmental substances microbes. CD200R1 an...
A mutation in the rat GIMAP5 gene predisposes for autoimmunity, most famously BB model of autoimmune type 1 diabetes mellitus. This is associated with severe peripheral T lymphopenia, as same mice, but mechanism by which normally protects cells from death unknown. a putative small GTPase, class proteins often fulfil their functions vicinity cellular membranes. The objective this study was to determine normal intracellular location lymphoid cells. Combining studies rat, mouse and human...
Breathing allows a multitude of airborne microbes and microbial compounds to access the lung. Constant exposure pulmonary microenvironment immunogenic particles illustrates need for proper control mechanisms ensuring differentiation between threatening harmless encounters. Discrimination live dead bacteria has been suggested be such mechanism. In this study, we performed infection studies murine precision cut lung slices (PCLS) with or heat-killed P. aeruginosa, in order investigate role...
The ability of SARS-CoV to infect different species, including humans, dogs, cats, minks, ferrets, hamsters, tigers, and deer, pose a continuous threat human animal health. Pigs, though closely related seem be less susceptible SARS-CoV-2. Former in vivo studies failed demonstrate clinical signs transmission between pigs, while later attempts using higher infectious dose reported viral shedding seroconversion. This study investigated species-specific cell susceptibility, virus dose-dependent...
Abstract The glycocalyx is a proteoglycan-rich layer present on the surface of all mammalian cells that particularly prevalent endothelial lining vasculature. It has been hypothesized mediates leukocyte migration by masking adhesion molecules and reducing to endothelium. Leukocyte recruitment key driver inflammatory diseases, including chronic skin disease, psoriasis. Here, we show leukocytes express heparan sulfate, an important component, their cell which lost in response psoriasis-like...
Traditional rehabilitation for hemiplegia is not necessarily based on a supported theoretical foundation and some evidence questiones the efficacy of current practice. The uncertainty relating to underlying theories serious issue, henceforth there has been move base treatment strategies scientific foundations which incorporate knowledge human learning mechanisms accompanying neurobiological processes. In this paper we argue that constraint induced movement therapy potentially very effective...
Abstract Background GIMAP (GTPase of the immunity-associated protein family) proteins are a family putative GTPases believed to be regulators cell death in lymphomyeloid cells. GIMAP1 was first reported member this gene family, identified as up-regulated at RNA level spleens mice infected with malarial parasite, Plasmodium chabaudi . Methods A monoclonal antibody against mouse developed and used analyse expression endogenous tissues normal defined sub-populations cells prepared from lymphoid...
The leukocyte-specific tyrosine phosphatase, CD45, severely impacts T cell development and activation by modulating TCR signaling. CD45-deficient (CD45KO) mice have reduced peripheral numbers where CD8 cells are underrepresented. In this article, we show that CD45KO unable to support efficient homeostatic proliferation, affecting more than CD4 cells. Using CD45-RAG1 double-deficient (45RAGKO) mice, lymphopenia-induced proliferation (LIP) of CD45-sufficient is defective in a host environment...
Abstract Introduction The skin immune system is tightly regulated to prevent inappropriate inflammation in response harmless environmental substances. This regulation actively maintained by mechanisms including cytokines and cell surface receptors its loss results inflammatory disease. In the case of psoriasis, activation leads IL‐17‐driven chronic inflammation, but molecular underlying this are not well understood. Immunoglobulin family member CD200 receptor, CD200R1, important regulators...
Abstract The skin immune system is tightly regulated to prevent inappropriate inflammation in response harmless environmental substances. This regulation actively maintained by mechanisms including cytokines and cell surface receptors its loss results inflammatory disease. In the case of psoriasis, activation leads IL-17-driven chronic inflammation, but molecular underlying this are not well understood. We reveal that immunoglobulin superfamily member CD200, signalling via receptor, CD200R1...
Abstract Psoriasis is a common chronic inflammatory skin disease with no cure. It driven by the IL-23/IL-17A axis and T H 17 cells but, recently group 3 innate lymphoid (ILC3s) have also been implicated. However, development, factors regulating activity of ILC3s remain incompletely understood. Immune regulatory pathways are particularly important at barrier sites such as skin, gut lung, which exposed to environmental substances microbes. CD200R1 an immune cell surface receptor inhibits...
Abstract Background Psoriasis and its associated inflammatory arthritis Psoriatic Arthritis (PsA) are potentially life-ruining conditions with numerous comorbidities. A previously-identified genetic risk association for psoriasis PsA lies in a non-coding region at chromosome 1p36.23, as such functional validation is required to determine the mechanism contributing psoriatic disease risk. Results rs11121131 – variant tight linkage rs11121129, lead GWAS 1p36.23 putative enhancer active...
Abstract Lymphopenia-induced T cell proliferation (LIP) occurs naturally in neonates and patients with depleted numbers due to infection or immunosuppression. LIP response an interaction between TCR self-MHC as well cytokines such IL-7 IL-15. However, the precise factors that induce limit are not understood. CD45 is a leukocyte specific tyrosine phosphatase reduces signaling threshold cells, modulates TLR dendritic cells can negatively regulate cytokine leukocytes. Given deficient mice have...
Abstract The aim of this study was to identify and validate a sensitive, high-throughput cost-effective SARS-CoV-2 RT-PCR assay be used as surveillance diagnostic tool for in University program. We conducted side-by-side clinical evaluation newly developed multiplex (EZ-SARS-CoV-2 Real-Time RT-PCR) with the commercial TaqPath COVID-19 Combo kit, which has an Emergency Use Authorization from FDA. EZ-SARS-CoV-2 incorporates two assays targeting N gene, internal control human RNase P PCR...