A5012685122- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Ion Transport and Channel Regulation
- interferon and immune responses
- Digital Economy and Work Transformation
- Adenosine and Purinergic Signaling
- Calcium signaling and nucleotide metabolism
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Education and Technology Integration
- Cell death mechanisms and regulation
- Construction Engineering and Safety
- Digital Radiography and Breast Imaging
- Protein Interaction Studies and Fluorescence Analysis
- Education Methods and Practices
- Nursing Education, Practice, and Leadership
- Gender, Feminism, and Media
- Reproductive tract infections research
- Nanofabrication and Lithography Techniques
- Breast Lesions and Carcinomas
University of Cambridge
2024
Babraham Institute
1996-2014
University of the West of England
2014
McGill University
2009
Oncor (United States)
2001
National Cancer Institute
1990
University of Oxford
1987
John Radcliffe Hospital
1987
Boehringer Ingelheim (United States)
1987
National Heart Lung and Blood Institute
1984
Integrin alphaEbeta7 is expressed almost exclusively by mucosal T cells and dendritic antigen-presenting (APCs) thought to be induced locally transforming growth factor-beta (TGF-beta). In mice, mRNA for the alphaE subunit was found abundant in but absent from other tissues. Exposure of a T-cell line TGF-beta strongly up-regulated levels within 30 min, nuclear run-on experiments established that regulation occurred at level transcription. The organization human gene very closely linked novel...
SENSORY INFORMATION AVAILABLE WHEN MUSICIANS' fingers arrive on instrument keys contributes to temporal accuracy in piano performance (Goebl & Palmer, 2008). The hypothesis that timing is related sensory (tactile) information available at finger-key contact was extended clarinetists' finger movements during key depressions and releases that, together with breathing, determine the of tone onsets. Skilled clarinetists performed melodies different tempi a synchronization task while their...
A mutation in the rat GIMAP5 gene predisposes for autoimmunity, most famously BB model of autoimmune type 1 diabetes mellitus. This is associated with severe peripheral T lymphopenia, as same mice, but mechanism by which normally protects cells from death unknown. a putative small GTPase, class proteins often fulfil their functions vicinity cellular membranes. The objective this study was to determine normal intracellular location lymphoid cells. Combining studies rat, mouse and human...
G imap3 ( IAN 4) and imap5 5) are highly homologous GTP ‐binding proteins of the imap family. imap5, whose transcripts abundant in mature lymphocytes, can associate with antiapoptotic B cl‐2 family proteins. While it is established that regulates T ‐cell survival, vivo role unclear. Here we report preparation characteristics mouse strains lacking and/or imap5. We found number cells was markedly reduced mice deficient both The defects cellularity were more severe than only No detected imap3,...
The GIMAPs (GTPases of the immunity-associated proteins) are a family small GTPases expressed prominently in immune systems mammals and other vertebrates. In mammals, studies mutant or genetically-modified rodents have indicated important roles for GIMAP development survival lymphocytes. No clear picture has yet emerged, however, molecular mechanisms by which they perform their function(s). Using biotin tag-affinity purification we identified major, highly specific, interaction between human...
Reports suggest that two members of the novel immune-associated nucleotide (Ian) GTPase family, Ian1 and Ian5, play roles in T cell development. We performed real-time PCR analysis expression Ian genes rat during maturation, macrophages lines. found all were expressed at relatively low levels early double-negative thymocyte stage but more strongly later stages. Our study also revealed fact previously reported Ian9, Ian10 Ian11 are, instead, parts a single gene for which we retain name...
Abstract The Gimap/IAN family of GTPases has been implicated in the regulation cell survival, particularly lymphomyeloid cells. Prosurvival and prodeath properties have described for different members. We generated novel serological reagents to study expression rats member Gimap4 (IAN1), which is sharply up-regulated at or soon after stage T cell-positive selection thymus. During these investigations we were surprised discover a severe deficiency inbred Brown Norway (BN) rat. Genetic...
Nucleic acid amplification tests, with their ability to detect very small amounts of nucleic acid, have become the principle diagnostic tests for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) in many sexual health clinics. The aim this study was investigate extent surface contamination CT GC within a city centre clinic evaluate potential containers used collection self-taken swabs.Surface assessed by systematically sampling 154 different sites one using transcription-mediated...
We have investigated the importance of dimerization E-cadherin in heterophilic adhesive interaction between and integrin α<sub>E</sub>β<sub>7</sub>. Dimerization cadherin molecules parallel alignment is known to be essential for homophilic adhesion has been attributed Ca<sup>2+</sup>-dependent interactions domain 1-2 junction or cross-intercalation Trp2 from one molecule other. disrupted either both these proposed mechanisms by point mutations E-cadherin-Fc tested modified proteins...
Background GTPases of the immunity-associated protein family (GIMAPs) are predominantly expressed in mature lymphocytes. Studies rodents deficient GIMAP1, GIMAP4, or GIMAP5 have demonstrated that these regulate lymphocyte survival. In contrast to other members, GIMAP8 contains three potential GTP-binding domains (G-domains), a highly unusual feature suggesting novel function for this protein. To examine role biology we examined expression during development. We also generated mouse and...
Reverse transcription-PCR (RT-PCR) techniques were used to identify the expression of ryanodine receptor (RyR) isoforms in gut epithelial cells. Restriction digest and sequence analysis PCR product showed presence RyR 2 3. [3H]Ry binding studies on a microsome preparation, high-salt buffer, specific with an EC50 15 microM. In order determine potential functional role for these RyRs, we first characterized response cells acetylcholine. At all concentrations acetylcholine induced sinusoidal...
Abstract Background GIMAP (GTPase of the immunity-associated protein family) proteins are a family putative GTPases believed to be regulators cell death in lymphomyeloid cells. GIMAP1 was first reported member this gene family, identified as up-regulated at RNA level spleens mice infected with malarial parasite, Plasmodium chabaudi . Methods A monoclonal antibody against mouse developed and used analyse expression endogenous tissues normal defined sub-populations cells prepared from lymphoid...
Automated decision-making systems are commonly used by human resources to automate recruitment decisions. Most automated utilize machine learning screen, assess, and give recommendations on candidates. Algorithmic bias prejudice common side-effects of these technologies that result in data-driven discrimination. However, proof this is often unavailable due the statistical complexities operational opacities learning, which interferes with abilities complainants meet requisite causal...
With the rise of algorithmic management, deployment AI surveillance has proliferated in modern workplace. relies on advanced computational methods to draw statistical inferences about workers from their data. These are subsequently used by employers inform various organisational and managerial decisions. This commodifies into entities, which objectifies instrumentalises value human work as a series data points for analysis. The article explores how this transformation impacts precarious role...