A5056217094- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Parvovirus B19 Infection Studies
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Molecular Biology Techniques and Applications
- Hepatitis C virus research
- Cutaneous lymphoproliferative disorders research
- Virology and Viral Diseases
- Metabolomics and Mass Spectrometry Studies
- Zoonotic diseases and public health
- T-cell and Retrovirus Studies
- Hepatitis B Virus Studies
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Biosensors and Analytical Detection
- Poxvirus research and outbreaks
Cornell University
2011-2024
New York State College of Veterinary Medicine
2011-2023
United States Department of Agriculture
2021
University of Illinois Urbana-Champaign
2021
Chicago Zoological Society
2021
Wildlife Conservation Society
2021
Nationwide Children's Hospital
2018
North Carolina State University
2018
Columbia University
2018
University of Copenhagen
2018
The human-animal-environment interface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important aspect the disease 2019 (COVID-19) pandemic that requires robust One Health-based investigations. Despite this, few reports describe natural infections in animals or directly link them to human using genomic data. In present study, we first cases SARS-CoV-2 infection tigers and lions United States provide epidemiological genetic evidence for human-to-animal transmission...
The highly pathogenic avian influenza (HPAI) H5N1 virus clade 2.3.4.4b has caused the death of millions domestic birds and thousands wild in USA since January 2022 (refs.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) emerged as the cause of a global pandemic in 2019–2020. In March 2020, New York City became epicenter United States for pandemic. On 27 Malayan tiger (Panthera tigris jacksoni) at Bronx Zoo developed cough and wheezing with subsequent inappetence. Over next week, an additional two Amur tigers altaica) same building three lions leo krugeri) separate also ill. The index case was anesthetized diagnostic workup. Physical examination...
Infections with the highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.4b virus have resulted in death of millions domestic birds and thousands wild U.S. since January, 2022. Throughout this outbreak, spillovers to mammals been frequently documented. Here, we report detection HPAI dairy cattle herds across several states The affected cows displayed clinical signs encompassing decreased feed intake, altered fecal consistency, respiratory distress, milk production abnormal milk....
Abstract Equine serum hepatitis (i.e., Theiler’s disease) is a serious and often life-threatening disease of unknown etiology that affects horses. A horse in Nebraska, USA, with died 65 days after treatment equine-origin tetanus antitoxin. We identified an parvovirus liver the dead administered The equine parvovirus-hepatitis (EqPV-H) shares <50% protein identity its phylogenetic relatives genus Copiparvovirus. Next, we experimentally infected 2 horses using antitoxin contaminated EqPV-H....
Background Three flaviviruses (equine pegivirus [EPgV]; Theiler's disease–associated virus [TDAV]; non‐primate hepacivirus [NPHV]) and equine parvovirus (EqPV‐H) are present in blood products; the TDAV, NPHV, EqPV‐H have been suggested as potential causes of serum hepatitis. Objective To determine prevalence these viruses horses with Animals Eighteen diagnosed hepatitis, enrolled from US referral hospitals. Methods In prospective case study, liver, serum, or both samples were tested for...
Equine parvovirus-hepatitis (EqPV-H) has recently been associated with cases of Theiler's disease, a form fulminant hepatic necrosis in horses. To assess whether EqPV-H is the cause we first demonstrated hepatotropism by PCR on tissues from acutely infected We then experimentally inoculated horses and 8 10 developed hepatitis. One horse showed clinical signs liver failure. The onset hepatitis was temporally seroconversion decline viremia. Liver histology situ hybridization lymphocytic...
The host range of SARS-CoV-2 and the susceptibility animal species to virus are topics great interest international scientific community. angiotensin I converting enzyme 2 (ACE2) protein is major receptor for virus, sequence structural analysis has been performed determine its cross-species conservation. Based on these analyses, cattle have implicated as a potential susceptible reported increased ACE2 distribution in liver kidney, lower levels lungs. goal current study was utilizing...
Avian influenza H5N1 is a highly pathogenic virus that primarily affects birds. However, it can also infect other animal species, including mammals. We report the infection of nine juvenile red foxes (Vulpes vulpes) with Highly Pathogenic Influenza A type (Clade 2.3.4.4b) in spring 2022 central, western, and northern regions New York, USA. The displayed neurologic signs, examination brain lung tissue revealed lesions, lesions ranging from moderate to severe meningoencephalitis. Analysis...
Equine herpesvirus (EHV) 1 and EHV-4 are common viral pathogens of horses that can cause upper respiratory disease, neurological abortion, or death. As characteristic alphaherpesviruses, both EHV-1 establish latency, resulting in a lifelong carrier state infected animals. Here we describe the development validation rapid sensitive multiplex real-time PCR assay (EHV1-4MP) simultaneously detects includes an endogenous internal control targeting equid genome. The EHV1-4MP analytical sensitivity...
To determine which respiratory viruses circulate among confined dogs, we analyzed nasal and pharyngeal swab specimens from shelter dogs with acute disease. An unknown virus was isolated. Monoclonal antibody testing indicated that it probably a pneumovirus. PCR sequence analysis closely related to murine
Background A novel equine parvovirus (EqPV‐H) was recently discovered in the liver with Theiler's disease. Objectives To determine prevalence of EqPV‐H infection naturally occurring disease cases and in‐contact horses absence historical biologic product administration. Animals Ten from 6 separate properties were included study, based on criteria acute onset clinical signs failure laboratory or histopathologic findings characteristic no history receiving an within preceding 4 months. In...
Abstract We describe the first cases of natural SARS-CoV-2 infection detected in animals United States. In March 2020, four tigers and three lions at Bronx Zoo developed mild respiratory signs. RNA was by rRT-PCR secretions and/or feces from all seven affected animals; viral antibodies were their keepers. isolated or situ hybridization co-localized with cellular damage. Whole genome sequence haplotype network analyses showed infected two different strains, suggesting independent...
The aim of this study was to identify and validate a sensitive, high-throughput, cost-effective SARS-CoV-2 real-time RT-PCR assay be used as surveillance diagnostic tool for in university program. We conducted side-by-side clinical evaluation newly developed multiplex (EZ-SARS-CoV-2 Real-Time RT-PCR) with the commercial TaqPath COVID-19 Combo Kit, which has an Emergency Use Authorization from FDA. EZ-SARS-CoV-2 incorporates two assays targeting N gene, internal control human RNase P PCR...
Equine parvovirus-hepatitis (EqPV-H) has been proposed as the aetiological cause of Theiler's disease, also known serum hepatitis. EqPV-H-associated disease not previously reported in Europe.To determine whether EqPV-H infection was associated with a 2018-2019 outbreak four horses on studfarm.Descriptive case series.The medical records from same farm diagnosed fatal were examined retrospectively. Information collected included clinical history, physical examination findings, tetanus...
In the present study, we assessed diagnostic sensitivity and determined viral RNA load infectivity of SARS-CoV-2 in paired respiratory (nasopharyngeal anterior nares) oral samples (saliva sublingual swab). Samples were collected from 77 individuals which 75 diagnosed with COVID-19 classified as symptomatic (n = 29), asymptomatic 31), or postsymptomatic 15). Specimens at one time point each individual, between day 1 23 after initial diagnosis, included self-collected saliva (S), (SL) swab,...
Schoolyards and suburban parks are two environments where active tick surveillance may inform local management approaches. Even in a state such as New York with robust programme operated by the Department of Health, these settings not routinely covered. The goal this study was to highlight importance for tick-borne pathogens describing their prevalence ticks collected from schoolyards guide use integrated pest settings. Tick dragging performed three regions State: Long Island, Lower Hudson...
Since the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), susceptibility animals and their potential to act as reservoirs or intermediate hosts for virus has been significant interest. Pigs are susceptible multiple coronaviruses have used an animal model other human infectious diseases. Research groups experimentally challenged swine with SARS-CoV-2 isolates results suggesting limited no viral replication. For this study, a isolate obtained from tiger which is...
A serological COVID-19 Multiplex Assay was developed and validated using serum samples from convalescent patients those collected prior to the 2020 pandemic. After initial testing of multiple potential antigens, SARS-CoV-2 nucleocapsid protein (NP) receptor-binding domain (RBD) spike were selected for human Assay. comparison synthesized mammalian expressed RBD proteins revealed clear advantages expression. Antibodies directed against NP strongly correlated with virus neutralization assay...
Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak hemorrhagic gastroenteritis following stay in pet housing facility. Routine diagnostic investigations failed to identify specific cause. Virus isolation from fresh necropsy tissues yielded calicivirus with sequence homology vesivirus within the group colloquially known as 2117 strains that were originally identified contaminants CHO cell bioreactors. In situ hybridization and reverse transcription-PCR...
Nanoliter scale real-time PCR uses spatial multiplexing to allow multiple assays be run in parallel on a single plate without the typical drawbacks of combining reactions together. We designed and evaluated panel based this principle rapidly identify presence common disease agents dogs horses with acute respiratory illness. This manuscript describes nanoscale diagnostic workflow for sample preparation, amplification, analysis target pathogen sequences, focusing procedures that are different...
Granulomatous mural folliculitis (GMF) is an uncommon reaction pattern occasionally observed in nonadapted ruminant hosts infected with malignant catarrhal fever viruses. This report characterizes GMF and concurrent cutaneous lesions 16 goats crusting dermatitis using histochemistry including hematoxylin eosin, periodic acid–Schiff, Grocott’s methenamine silver, immunohistochemistry for CD3, CD20, ionized calcium binding adaptor molecule 1, cytokeratin AE1/3. Infiltrates all cases consisted...
Abstract Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) emerged as the cause of a global pandemic in 2019-2020. In March 2020 New York City became USA epicenter for pandemic. On 27, Malayan tiger ( Panthera tigris jacksoni ) at Bronx Zoo developed cough and wheezing with subsequent inappetence. Over next week, an additional two Amur tigers P. t. altaica same building three lions leo krugeri separate also ill. The index case was immobilized, physical examination bloodwork...
Epizootic mortalities in American Crows (Corvus brachyrhynchos) during the winter months, referred to as mortality of crows, have been recorded North America for almost two decades. The most common postmortem findings include necrotizing enteritis, colitis, and fibrinous splenic necrosis. These are proposed be due infection with a Reovirus sp. Our objectives were characterize pathology seasonality epizootics New York State (NYS), confirm causative role an Orthoreovirus sp., determine its...