A5090498974- Amphibian and Reptile Biology
- Turtle Biology and Conservation
- Aquaculture disease management and microbiota
- Viral Infections and Vectors
- Bird parasitology and diseases
- Microbial infections and disease research
- Coccidia and coccidiosis research
- Reproductive tract infections research
- Rabies epidemiology and control
- Antimicrobial Peptides and Activities
- Dermatological diseases and infestations
- Vector-borne infectious diseases
- Veterinary Oncology Research
- Virus-based gene therapy research
- Streptococcal Infections and Treatments
- Virology and Viral Diseases
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Insect and Arachnid Ecology and Behavior
- Species Distribution and Climate Change
- Animal and Plant Science Education
- Toxoplasma gondii Research Studies
- Vector-Borne Animal Diseases
- Study of Mite Species
- Genetic diversity and population structure
Long Island University
2020-2024
University of Wyoming
2024
Cornell University
2019-2022
New York State College of Veterinary Medicine
2019
Département Santé Animale
2019
Hampshire College
2019
Tufts University
2019
Vermont Fish & Wildlife Department
2019
University of New Hampshire
2019
New Hampshire Fish and Game Department
2019
Western palearctic salamander susceptibility to the skin disease caused by amphibian chytrid fungus Batrachochytrium salamandrivorans (Bsal) was recognized in 2014, eliciting concerns for a potential novel wave of declines following B. dendrobatidis (Bd) chytridiomycosis global pandemic. Although Bsal had not been detected North America, initial experimental trials supported heightened caudate amphibians chytridiomycosis, recognizing critical threat this pathogen poses American biodiversity...
The introduction of a new group dendrobatid frogs to an established captive amphibian collection was followed by several acute mortalities in both resident and introduced frog populations. Chytridiomycosis, caused Batrachochytrium dendrobatidis, diagnosed histology two the dead frogs. Following diagnosis, all amphibians were moved specially made quarantine room with strict handling protocols treated itraconazole. Frogs, being terrestrial amphibians, itraconazole (Sporanox™, 10 mg/ml) at...
Wood frogs ( Rana sylvatica) are highly susceptible to infection with Frog virus 3 (FV3, Ranavirus, Iridoviridae), a cause of mass mortality in wild populations. To elucidate the pathogenesis FV3 wood frogs, 40 wild-caught adults were acclimated captivity, inoculated orally fatal dose 104.43 pfu/frog, and euthanized at 0.25, 0.5, 1, 2, 4, 9, 14 days postinfection (dpi). Mild lesions occurred sporadically skin (petechiae) bone marrow (necrosis) during first 2 dpi. Severe 1 weeks consisted...
Amphibian populations suffer massive mortalities from infection with frog virus 3 FV3, genus Ranavirus, family Iridoviridae, a pathogen also involved in of fish and reptiles. Experimental oral FV3 captive-raised adult wood frogs, Rana sylvatica Lithobates sylvaticus, was performed as the first step establishing native North American animal model ranaviral disease to study pathogenesis host response. Oral dosing successful LD50 102.93 2.423.44 p.f.u. for frogs averaging 35mm length. Onset...
Abstract Bald eagles ( Haliaeetus leucocephalus ) are considered a recovery success in the United States after rebounding from near extirpation due to widespread use of insecticide dichlorodiphenyltrichloroethane (DDT) twentieth century. Although abundances bald have increased since DDT was banned, other contaminants remained environment with unknown influence on eagle population trends. Ingestion spent lead (Pb) ammunition, source Pb most available and scavengers States, is known kill...
There is increasing public interest in the overall health of marine environment. Harbor porpoises Phocoena phocoena have a coastal distribution, and stranded animals function as sentinels for population ecosystem health. The goal this retrospective study was to join datasets from western Atlantic eastern Pacific coasts Canada investigate causes morbidity mortality species. A total 241 necropsy records were reviewed including 147 (61%) region 94 (39%) 1988 2011. cause death could be...
Collection of blood from amphibians, as in other classes vertebrate animals, is essential to evaluate parameters health, diagnose hemoparasitism, identify viral and bacterial pathogens, measure antibodies. Various methods collection have been described for amphibians. Most can be cumbersome (venipucture femoral vein, ventral abdominal vein or lingual venus plexus) result pain deleterious health consequences (cardiac puncture toe-clipping). We describe an easy practical technique collect...
Finch trichomonosis, caused by Trichomonas gallinae, emerged in the Canadian Maritime provinces 2007 and has since ongoing mortality regional purple finch (Carpodacus purpureus) American goldfinch (Carduelis tristis) populations. gallinae was isolated from (1) finches rock pigeons (Columbia livia) submitted for post-mortem or live-captured at bird feeding sites experiencing trichomonosis mortality; (2) seed these same sites; (3) known roosts humanely killed. Isolates were characterized using...
Intratesticular injection of EsterilSol (zinc gluconate neutralized with arginine) is a chemical sterilant for male dogs sometimes used in population control campaigns. Adverse reactions have been reported 1% to 4% treated dogs, but detailed histomorphologic descriptions are lacking. During behavioral study conducted the Chilean Patagonia 2012, severe necrosuppurative orchitis and ulcerative dermatitis were observed 2 36 (6%) sterilized according manufacturer’s instructions. Reactions noted...
Background The African frog, Xenopus tropicalis, is widely used in biomedical and toxicologic research. Reference intervals ( RI ) for hematologic variables, valuable to research health status assessment, have not been established. Objectives purpose of the study was determine X establish whether automated cell counting can facilitate routine assessment frogs. Methods Blood from 41 adult healthy tropicalis collected via cardiac puncture, diluted Natt–Herrick solution. Complete WBC , RBC...
Ranavirus (Iridoviridae) infection is a significant cause of mortality in amphibians. Detection infected individuals, particularly carriers, necessary to prevent and control outbreaks. Recently, the use toe clips detect ranavirus through PCR was proposed as an alternative more frequently used lethal liver sampling green frogs (Rana [Lithobates] clamitans). We attempted reevaluate clips, evaluate potential blood onto filter paper hepatic fine needle aspirates (FNAs) further alternatives,...
Taenia crassiceps is a parasite of wild canids and dogs that serve as definite hosts, harboring the adult cestode, whereas rodents are intermediate hosts in which metacestode/cysticercus/larval stage occurs. Fecal-oral transmission ensures parasite's lifecycle. At times, humans act accidental hosts. Despite public health concern this warrants, its epidemiology remains unclear. In report, we document occurrence metacestodes T. muskrat (Ondatra zibethicus) domestic dog from northeastern United...
Trichomonas gallinae has emerged worldwide as a cause of mortality in songbirds (passerines). The congregation numerous birds, including the reservoir hosts, pigeons and doves (columbids), at backyard feeding watering sources been suggested potential driver for outbreaks. Evidence supporting role water transmission established, but birdseed trichomoniasis remained to be investigated. We assessed survival T. three commercial birdseeds (mixed seed, black-oil sunflower niger seed) routinely...
is a promiscuous pathogen infecting many vertebrates, including humans, whose persistent infections are problematic for aquaculture and public health. Among unsettled aspects of host-pathogen interactions, the respective roles conventional innate-like T (iT) cells in host defenses against
Juvenile eastern water dragons ( Intellagama lesueurii lesueurii) are highly susceptible to infection with Bohle iridovirus (BIV), a species of ranavirus first isolated from ornate burrowing frogs in Townsville, Australia. To investigate the progression BIV dragons, 11 captive-bred juveniles were orally inoculated dose 10 4.33 TCID 50 and euthanized at 3, 6, 8, 10, 12, 14 days postinfection (dpi). Viral DNA was detected via polymerase chain reaction (PCR) liver, kidney, cloacal swabs 3 dpi....
Ranavirus is the second most common infectious cause of amphibian mortality. These viruses affect caudates, an order in which information regarding pathogenesis scarce. In Netherlands, two strains (CMTV-NL I and III) were suspected to possess distinct pathogenicity based on field data. To investigate susceptibility disease progression urodeles determine differences between strains, 45 adult smooth newts (Lissotriton vulgaris) challenged via bath exposure with these ranaviruses their...
Three fishers (Martes pennanti), 2 gray foxes (Urocyon cinereoargenteus), 1 mink (Neovison vison), skunk (Mephitis mephitis), and raccoon (Procyon lotor), from Vermont New Hampshire, had lesions on autopsy consistent with canine distemper virus (CDV) infections diagnosed in a 12-mo period 2016-2017. Lesions of CDV infection were most commonly noted the lungs (8 8 animals), urothelium (5 8), biliary tract gastrointestinal (4 7), brain 6). Splenic seen 3 animals. The diagnosis was confirmed...
A hatching-year bald eagle (Haliaeetus leucocephalus) was presented for clinical examination after being found unable to fly. Upon admission, routine wet-mount microscopy detected no trichomonads. Five months later, oral cavity inspection abnormalities, but the swabbed research on trichomonosis in maritime birds. The swab used inoculate an InPouch TF culture and trichomonads were visible within 24 hr. Genotyping (ITS) revealed a Trichomonas isolate that 100% identical from bearded vulture...
Background Although the Wood Frog, Rana sylvatica , is used in research on infectious diseases of amphibians, hematologic RI s or response to infection have not been established. Objectives The purpose study was determine for adult Frogs and alterations associated with Frog Virus 3 ( FV 3, Ranavirus sp.). Methods Blood collected from 40 wild‐caught that had captivity 6 months. Complete (Natt–Herrick solution hemocytometry) differential (Wright–Giemsa‐stained smears) WBC RBC thrombocyte cell...
Investigation of mortalities in isolated wild amphibian populations presents diagnostic difficulties that can hinder reaching a definitive diagnosis for the cause death. Disease only be diagnosed when pathogen presence (e.g. detection by PCR) is linked to tissue lesions (histopathology) host. We report 2-site outbreak ranavirosis anuran tadpoles boreal forest Wood Buffalo National Park, Canada, histologic and molecular techniques. Mortalities occurred wood frog Rana sylvatica chorus...
The ranavirus Frog Virus 3 (FV3) and the chytrid fungus Batrachochytrium dendrobatidis ( Bd) are significant contributors to global amphibian declines both pathogens target skin. We previously showed that tadpoles adults of anuran Xenopus laevis express notable levels granulocyte chemokine genes cxcl8a cxcl8b) within their skin likely possess skin-resident granulocytes. Presently, we show tadpole adult X. indeed granulocyte-lineage cells epidermises distinct from mast cells, which found...
Chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd), has resulted in decline or extinction of approximately 200 frog species worldwide. It been reported throughout much North America, but its presence on Prince Edward Island (PEI), eastern coast Canada, was unknown. To determine and prevalence Bd PEI, skin swabs were collected from 115 frogs 18 separate sites across province during summer 2009. The tested through single round end-point PCR for DNA. Thirty-one positive,...